1887

Abstract

SUMMARY: -Arabinose is broken down by MNF300 via 2-oxoglutarate semialdehyde. Enzyme activities in cells grown on succinate, mannitol or arabinose indicated much greater modulation of arabinonate dehydratase, 2-keto-3-deoxyarabinonate dehydratase and 2-oxoglutarate semialdehyde dehydrogenase than of arabinose dehydrogenase or of arabinono-γ-lactonase. In cowpea NGR234, all the enzymes of -arabinose metabolism except -arabinono-γ-lactonase were inducible. Assays for such enzymes in snake bean bacteroids indicated that -arabinose did not reach the bacteroids in large quantities. The Tn-induced mutant MNF3045 of was unable to grow on -arabinose and accumulated -arabinono-γ-lactone and -arabinonate. Product accumulation and enzyme assays suggested that this mutant was defective in -arabinonate dehydratase. It nodulated peas and the nodules fixed N, indicating that the supply of -arabinose is not essential for bacteroid function. Another Tn-induced mutant of , MNF3041, lacked ribokinase and was unable to grow on -ribose; this mutant was also able to nodulate peas and fix N.

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1986-10-01
2021-08-01
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References

  1. Abdel-Akher M., Smith F. 1951; The detection of carbohydrate esters and lactones after separation by paper chromatography. Journal of the American Chemical Society 73:5859–5860
    [Google Scholar]
  2. Adams E. 1971; Enzymes and intermediates of hydroxyproline degradation. Methods in Enzymology 17B:266–306
    [Google Scholar]
  3. Arwas R., McKay I. A., Rowney P., Dilworth M. J., Glenn A. R. 1985; Properties of organic acid utilization mutants of Rhizobium leguminosarum strain 300. Journal of General Microbiology 131:2059–2066
    [Google Scholar]
  4. Ashwell G. 1957; Colorimetric analysis of sugars. Methods in Enzymology 3:73–105
    [Google Scholar]
  5. Berg D. E., Egner C., Lowe J. B. 1983; Mechanism of F-factor enhanced excision of transposon Tn5. Gene 22:1–7
    [Google Scholar]
  6. Beringer J. E. 1974; R factor transfer in Rhizobium leguminosarum. Journal of General Microbiology 84:188–198
    [Google Scholar]
  7. Beringer J. E., Beynon J. L., Buchanan-Wollaston A. V., Johnston A. W. B. 1978; Transfer of the drug-resistance transposon Tn5 to Rhizobium. Nature, London 276:633–634
    [Google Scholar]
  8. Brown C. M., Dilworth M. J. 1975; Ammonia assimilation by Rhizobium cultures and bacteroids. Journal of General Microbiology 86:39–48
    [Google Scholar]
  9. Buchanan-Wollaston A. V. 1979; Generalized transduction in Rhizobium leguminosarum. Journal of General Microbiology 112:135–142
    [Google Scholar]
  10. Cavallini D., Frontali N., Toschi G. 1949; Determination of keto-acids by partition chromatography on filter paper. Nature, London 163:568–569
    [Google Scholar]
  11. Chatterjee A. K., Thurn K. K., Feese D. A. 1983; Tn5 induced mutations in the enterobacterial phytopathogen Erwinia chrysanthemi. Applied and Environmental Microbiology 45:644–650
    [Google Scholar]
  12. Dagley S., Trudgill P. W. 1965; The metabolism of galactarate, d-glucarate and various pentoses by species of Pseudomonas. Biochemical Journal 95:48–58
    [Google Scholar]
  13. Dilworth M. J. 1962; Oxygen inhibition in Azotobacter vinelandii. Pyruvate oxidation. Biochimica et biophysica acta 56:127–138
    [Google Scholar]
  14. Duncan M. J. 1979; l-Arabinose metabolism in rhizobia. Journal of General Microbiology 113:177–179
    [Google Scholar]
  15. Duncan M. J. 1981; Properties of Tn5-induced carbohydrate mutants in Rhizobium meliloti. Journal of General Microbiology 122:61–67
    [Google Scholar]
  16. Duncan M. J., Fraenkel D. G. 1979; α-Ketoglutarate dehydrogenase mutant of Rhizobium meliloti. Journal of Bacteriology 137:415–419
    [Google Scholar]
  17. Egner C., Berg D. E. 1981; Excision of transposon Tn5 is dependent on the inverted repeats but not on the transposase function of Tn5. Proceedings of the National Academy of Sciences of the United States of America 78:459–463
    [Google Scholar]
  18. Glenn A. R., Arwas R., McKay I. A., Dilworth M. J. 1984a; Fructose metabolism in wild-type, fructokinase-negative and revertant strains of Rhizobium leguminosarum. Journal of General Microbiology 130:231–237
    [Google Scholar]
  19. Glenn A. R., McKay I. A., Arwas R., Dilworth M. J. 1984b; Sugar metabolism and the symbiotic properties of carbohydrate mutants of Rhizobium leguminosarum. Journal of General Microbiology 130:239–245
    [Google Scholar]
  20. Hestrin S. 1949; The reaction of acetylcholine and other carboxylic acid derivatives with hydroxylamine, and its analytical application. Journal of Biological Chemistry 180:249–261
    [Google Scholar]
  21. Hockenhull D. J. D., Hunter G. D., Herbert M. W. 1953; Separation of α-keto acids by paper chromatography. Chemistry and Industry 127:
    [Google Scholar]
  22. Hudman J. F., Glenn A. R. 1980; Glucose uptake by free-living and bacteroid forms of Rhizobium leguminosarum. Archives of Microbiology 128:72–77
    [Google Scholar]
  23. Johnston A. W. B., Beringer J. E. 1975; Identification of the Rhizobium strains in pea root nodules using genetic markers. Journal of General Microbiology 87:343–350
    [Google Scholar]
  24. MacGee J., Doudoroff M. 1954; A new phosphorylated intermediate in glucose oxidation. Journal of Biological Chemistry 210:617–626
    [Google Scholar]
  25. McKay I. A., Glenn A. R., Dilworth M. J. 1985; Gluconeogenesis in Rhizobium leguminosarum MNF3841. Journal of General Microbiology 131:2067–2073
    [Google Scholar]
  26. Moore S., Link K. P. 1940; Carbohydrate characterization. I. The oxidation of aldoses by hypoiodite in methanol. II. The identification of seven aldomonosaccharides as benzimidazole derivatives. Journal of Biological Chemistry 133:293–311
    [Google Scholar]
  27. Novello F., McLean G. 1968; The pentose phosphate pathway of glucose metabolism. Biochemical Journal 107:775–791
    [Google Scholar]
  28. Pedrosa F. O., Zancan G. T. 1974; l-Arabinose metabolism in Rhizobium japonicum. Journal of Bacteriology 119:336–338
    [Google Scholar]
  29. Peterson J. B., LaRue T. A. 1982; Soluble aldehyde dehydrogenase and metabolism of aldehydes by soybean bacteroids. Journal of Bacteriology 151:1473–1484
    [Google Scholar]
  30. Reibach P. H., Mask P. L., Streeter J. 1981; A rapid one-step method for the isolation of bacteroids from root nodules of soybean plants, utilizing self-generating Percoll gradients. Canadian Journal of Microbiology 27:491–495
    [Google Scholar]
  31. Saroso S., Glenn A. R., Dilworth M. J. 1984; Carbon utilization by free-living and bacteroid forms of cowpea Rhizobium NGR234. Journal of General Microbiology 130:1809–1814
    [Google Scholar]
  32. Stoolmiller A. C. 1975; dl- and l-2-keto-3-deoxyarabonate. Methods in Enzymology 41B:101–103
    [Google Scholar]
  33. Stoolmiller A. C., Abeles R. H. 1966; Formation of α-ketoglutaric semialdehyde from l-2-keto-3-deoxyarabonic acid and isolation of l-2-keto-3-deoxyarabonate dehydratase from Pseudomonas saccharophila. Journal of Biological Chemistry 241:5764–5771
    [Google Scholar]
  34. Smith I. 1960 Chromatographic Techniques London: Heinemann;
    [Google Scholar]
  35. Stowers M. D. 1985; Carbon metabolism in Rhizobium species. Annual Review of Microbiology 39:89–108
    [Google Scholar]
  36. Virtanen A. I., Miettinen J. K., Kunttu H. 1953; α-Ketoacids in green plants. Acta chemica scandinavica 7:38–44
    [Google Scholar]
  37. Weimberg R. 1959; l-2-Keto-4,5-dihydroxyvaleric acid: an intermediate in the oxidation of l-arabinose by Pseudomonas saccharophila. Journal of Biological Chemistry 234:727–732
    [Google Scholar]
  38. Weissbach A., Hurwitz J. 1959; The formation of 2-keto-3-deoxyheptonic acid in extracts of Escherichia coli. Journal of Biological Chemistry 234:705–709
    [Google Scholar]
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