1887

Abstract

SUMMARY: Antibiotic-resistant and auxotrophic mutants of were isolated. These were used as recipients for the uptake from of broad-host-range R plasmids R68.45, RP1, and RP1 and RP4 carrying transposons Tn and Tn respectively. transconjugants from these crosses were used as donors to mobilize Str, Nal, and chromosomal markers to or to recipient strains. The frequency of plasmid transfer varied and depended on the donor and recipient strains used. Differences in chromosome mobilization frequencies of individual markers were observed and appeared to depend on the presence or absence of transposons Tn and Tn on the plasmid. Linkage was detected between the and Nal markers.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-132-10-2685
1986-10-01
2021-05-07
Loading full text...

Full text loading...

/deliver/fulltext/micro/132/10/mic-132-10-2685.html?itemId=/content/journal/micro/10.1099/00221287-132-10-2685&mimeType=html&fmt=ahah

References

  1. Achtman M., Willetts N., Clark A. J. 1971; Beginning a genetic analysis of conjugation transfer determined by the F factor in E. coli by isolation and characterisation of transfer deficient mutants. Journal of Bacteriology 106:529–538
    [Google Scholar]
  2. Al-Sallami S. F. M., Coote J. G., Parton R. 1981; Transformation of virulence with streptomycin resistance in Bordetella pertussis. Society for General Microbiology Quarterly 8:133
    [Google Scholar]
  3. Beringer J. E., Hopwood D. A. 1976; Chromosomal recombination and mapping in Rhizobium leguminosarum. Nature, London 264:291–294
    [Google Scholar]
  4. Beringer J. E., Hoggan S. A., Johnston A. W. B. 1978; Linkage mapping in Rhizobium leguminosarum by means of R plasmid-mediated recombination. Journal of General Microbiology 104:201–207
    [Google Scholar]
  5. Bradley D. E., Taylor D. E., Cohen D. R. 1980; Specification of surface mating systems among conjugative drug resistance plasmids in E. coli K12. Journal of Bacteriology 143:1466–1470
    [Google Scholar]
  6. Branefors P. 1964; Transformation of streptomycin resistance in Bordetella pertussis. Acta pathologica et microbiologica scandinavica 62:249–254
    [Google Scholar]
  7. Brownlie R. M., Coote J. G., Parton R. 1985; Adenylate cyclase activity during phenotypic variation of Bordetella pertussis. Journal of General Microbiology 131:27–38
    [Google Scholar]
  8. Burkardt H. J., Riess G., Puhler A. 1979; Relationship of group P-1 plasmids revealed by heteroduplex experiments: RP1, RP4, R68 and RK2 are identical. Journal of General Microbiology 114:341–348
    [Google Scholar]
  9. Clowes R. C., Hayes W. 1968 Experiments in Microbial Genetics p. 185 Oxford: Blackwell;
    [Google Scholar]
  10. Cohen S. M., Wheeler M. W. 1946; Pertussis vaccine prepared with phase I cultures grown in fluid medium. American Journal of Public Health 36:371–376
    [Google Scholar]
  11. Datta N., Hedges R. W. 1972; Host ranges of R factors. Journal of General Microbiology 70:453–460
    [Google Scholar]
  12. Dreyfus L. A., Iglewski B. H. 1985; Conjugation-mediated genetic exchange in Legionella pneumophila. Journal of Bacteriology 161:80–84
    [Google Scholar]
  13. Haas D., Holloway B. W. 1976; R factor variants with enhanced sex factor activity. Molecular and General Genetics 144:243–251
    [Google Scholar]
  14. Hamada S. E., Luckey J. P., Farrand S. K. 1979; R-plasmid mediated chromosomal gene transfer in Agrobacterium tumefaciens. Journal of Bacteriology 139:280–286
    [Google Scholar]
  15. Holloway B. W. 1979; Plasmids that mobilise the bacterial chromosome. Plasmid 2:1–19
    [Google Scholar]
  16. Imaizumi A., Suzuki Y., Ono S., Sato H., Sato Y. 1983; Heptakis (2,6-O-dimethyl)-β-cyclodextrin: a novel growth stimulant for Bordetella pertussis Phase I. Journal of Clinical Microbiology 17:781–786
    [Google Scholar]
  17. Kleckner N., Roth J., Botstein D. 1977; Genetic engineering in vivo using translocatable drug resistance elements. Journal of Molecular Biology 116:125–159
    [Google Scholar]
  18. Kloos W. E., Dobrogosz W. J., Ezzell J. W., Kimbro B. R., Manclark C. R. 1979; DNA–DNA hybridization, plasmids and genetic exchange in the genus Bordetella. In International Symposium on Pertussis pp. 70–80 Edited by Manclark C. R., Hill J. C. Washington, DC: US DHEW Publications no. (NIH);79–1830
    [Google Scholar]
  19. Lacey B. W. 1960; Antigenic modulation of B. pertussis. Journal of Hygiene 58:57–93
    [Google Scholar]
  20. Lichtenstein C., Brenner S. 1981; Site-specific properties of Tn7 transposition into the E. coli chromosome. Molecular and General Genetics 183:380–387
    [Google Scholar]
  21. Megias M., Caviedes M. A., Palomares A. J., Perez-Silva J. 1982; Use of plasmid R68.45 for constructing a circular linkage map of the Rhizobium trifolii chromosome. Journal of Bacteriology 149:59–64
    [Google Scholar]
  22. Parton R., Wardlaw A. C. 1975; Cell-envelope proteins of Bordetella pertussis. Journal of Medical Microbiology 8:47–57
    [Google Scholar]
  23. Pemberton J. M., Bowen A. R. St G. 1981; High frequency chromosome transfer in Rhodopseudomonas sphaeroides promoted by broad-host-range plasmid RP1 carrying mercury transposon Tn501. Journal of Bacteriology 147:110–117
    [Google Scholar]
  24. Pischl D. L., Farrand S. K. 1983; Transposon-facilitated chromosome mobilisation in Agrobacterium tumefaciens. Journal of Bacteriology 153:1451–1460
    [Google Scholar]
  25. Riess G., Holloway B. W., Puhler A. 1980; R68.45, a plasmid with chromosome mobilizing ability (Cma) carries a tandem duplication. Genetical Research 36:99–109
    [Google Scholar]
  26. Stainer D. W., Scholte M. J. 1971; A simple chemically-defined medium for the production of phase I Bordetella pertussis. Journal of General Microbiology 63:211–220
    [Google Scholar]
  27. Tatra P. K., Goodwin P. M. 1983; R-plasmid-mediated chromosome mobilization in the facultative methylotroph Pseudomonas AM1. Journal of General Microbiology 129:2629–2632
    [Google Scholar]
  28. Tucker W. T., Pemberton J. M. 1979; Conjugation and chromosome transfer in Rhodopseudomonas sphaeroides mediated by W and P group plasmids. FEMS Microbiology Letters 5:173–176
    [Google Scholar]
  29. Wardlaw A. C., Parton R. 1979; Changes in envelope protein and correlation with biological activities in B. pertussis. In International Symposium on Pertussis pp 94–98 Edited by Manclark C. R., Hill J. C. Washington, DC: DHEW Publication no. (NIH);79–1830
    [Google Scholar]
  30. Weiss A. A., Falkow S. 1982; Plasmid transfer in Bordetella pertussis; conjugation and transformation. Journal of Bacteriology 152:549–552
    [Google Scholar]
  31. Weiss A. A., Falkow S. 1983; Transposon insertion and subsequent donor formation promoted by Tn501 in Bordetella pertussis. Journal of Bacteriology 153:304–309
    [Google Scholar]
  32. Weiss A. A., Falkow S. 1984; Genetic analysis of phase change in Bordetella pertussis. Infection and Immunity 43:263–269
    [Google Scholar]
  33. Weiss A. A., Hewlett E. L., Myers G. A., Falkow S. 1983; Tn5-induced mutations affecting virulence factors in Bordetella pertussis. Infection and Immunity 42:33–41
    [Google Scholar]
  34. Weiss A. A., Hewlett E. L., Myers G. A., Falkow S. 1984; Pertussis toxin and extracytoplasmic adenylate cyclase as virulence factors of Bordetella pertussis. Journal of Infectious Diseases 150:219–222
    [Google Scholar]
  35. Willetts M. S., Crowther C., Holloway B. W. 1981; The insertion sequence IS21 of R68.45 and the molecular basis for mobilisation of the bacterial chromosome. Plasmid 6:30–52
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-132-10-2685
Loading
/content/journal/micro/10.1099/00221287-132-10-2685
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error