SUMMARY: Antibiotic-resistant and auxotrophic mutants of were isolated. These were used as recipients for the uptake from of broad-host-range R plasmids R68.45, RP1, and RP1 and RP4 carrying transposons Tn and Tn respectively. transconjugants from these crosses were used as donors to mobilize Str, Nal, and chromosomal markers to or to recipient strains. The frequency of plasmid transfer varied and depended on the donor and recipient strains used. Differences in chromosome mobilization frequencies of individual markers were observed and appeared to depend on the presence or absence of transposons Tn and Tn on the plasmid. Linkage was detected between the and Nal markers.


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