SUMMARY: We have determined the restriction and functional map of the ColE9-J plasmid. By sub-cloning and transposon mutagenesis we have shown that the ColE9 gene and the ColE5 gene present on the ColE9-J plasmid are located on separate RI fragments. Using an expression vector we have demonstrated the presence of two genes on the ColE9-J plasmid, both of which are dependent upon the colicin E9 structural gene promoter. Promoter mapping studies imply that the colicin E9 structural gene and the ColE5 gene are transcribed in the same direction, but that the ColE9 gene is transcribed in the opposite orientation.


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