1887

Abstract

SUMMARY: Surface charge and hydrophobicity of have been investigated in relation to phagocytosis by human polymorphonuclear leucocytes (PMNs) . The treponemal surface was relatively hydrophobic and negatively charged but despite these properties, phagocytosis, as assessed by luminol-enhanced chemiluminescence, was minimal in the absence of serum. Preopsonization of bacteria with serum reduced surface hydrophobicity but promoted phagocytosis, suggesting that specific immune mechanisms may be more important in controlling phagocytosis of than non-specific surface properties. evoked a much weaker chemiluminescence response from PMNs than the non-pathogenic treponeme biotype Reiterii even though similar numbers of bacteria were phagocytosed, suggesting differences in the reactivity of the surface components of the two organisms toward PMNs. The reactivity of towards PMNs could be increased by removal of the bacterial outer membrane by Triton X-100 treatment. These observations reinforce the suggestion that the outer surface of is inherently inert.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-132-1-133
1986-01-01
2022-01-17
Loading full text...

Full text loading...

/deliver/fulltext/micro/132/1/mic-132-1-133.html?itemId=/content/journal/micro/10.1099/00221287-132-1-133&mimeType=html&fmt=ahah

References

  1. Albertsson P.A., Andersson B., Larson C., Akerlund H.E. 1982; Phase partition - a method for purification and analysis of cell organelles and membrane vesicles. Methods of Biochemical Analysis 28:115–150
    [Google Scholar]
  2. Alderete J.F., Baseman J.B. 1979; Surface-associated host proteins on virulent Treponema pallidum. Infection and Immunity 26:1048–1056
    [Google Scholar]
  3. Bailey M.J., Penn C.W., Cockayne A. 1985; Evidence for the presence of lipopolysaccharide in Treponema phagetienis (biotype Reiterii) but not in T. pallidurn Nichols. FEMS Microbiology Letters 27:117–121
    [Google Scholar]
  4. Beachey E.H. (editor)1980; Bacterial adherence. In Receptors and Recognition Series B. London:. Chapman & Hall6
    [Google Scholar]
  5. Bridges C.G., Dasilva G.L., Yamamura M., Valdimarsson H. 1980; A radiometric assay for the combined measurement of phagocytosis and intracellular killing of Candida albicans . Clinical and Experimental Immunology 42:226–233
    [Google Scholar]
  6. Edebo L., Normann B., Skogh T., Stjernstrom I. 1980; Properties of antibodies of different immunoglobulin classes in relation to opsonization. Monographs in AIlergy 17:48–70
    [Google Scholar]
  7. Fitzgerald T.J., Johnson R.C., Ritzi D.M. 1979; Relationship of Treponema pallidum to acidic mucopolysaccharides. Infection and Immunity 24:252–260
    [Google Scholar]
  8. Hanff P.A., Miller J.N., Lovett M.A. 1979; Molecular characterization of common treponemal antigens. Infection and Immunity 40:825–828
    [Google Scholar]
  9. Hederstedt B. 1976; Studies on the Treponema pallidum immobilizing activity in normal human serum. 3. The kinetics of the immobilization reaction of normal and immune sera. Acta patholo-gica et microbiologica scandinavica, Section C 84:142–147
    [Google Scholar]
  10. Lukehart S.A., Miller J.N. 1978; Demonstration of the in vitro phagocytosis of Treponema pallidum by rabbit peritoneal macrophages. Journal qf Immunology 121:2014–2024
    [Google Scholar]
  11. Lukehart S.A., Baker-Zander S.A., Cheri-Lloyd R.M., Sell S. 1980; Characterization of lymphocyte responsiveness in early experimental syphilis. II. Nature of cellular infiltration and Treponema pallidum distribution in testicular lesions. Journal qf Immunology 124:461–467
    [Google Scholar]
  12. Magnusson H., Stendahl O., Tagesson C., Edebo L., Johansson G. 1977; The tendency of smooth and rough Salmonella typhimurium bacteria and lipopolysaccharide to hydrophobic and ionic interaction as studied in aqueous polymer two-phase systems. Acta pathologica et microbiologica scandinavica, Section B 85:212–218
    [Google Scholar]
  13. Miorner H., Albertsson P., Kronvall G. 1982; Isoelectric points and surface hydrophobicity of Gram-positive cocci as determined by crosspartition and hydrophobic affinity partition in aqueous two-phase systems. Infection and Immunity 36:227–234
    [Google Scholar]
  14. Musher D.M., Hague-Park M., Gyorkey F., Anderson D.C., Baughn R.H. 1983; The interaction between Treponema pallidum and human polymorphonuclear leukocytes. Journal of Injectious Diseases 147:77–86
    [Google Scholar]
  15. Ofek I., Whitnack E., Beachey E.H. 1983; Hydrophobic interactions of group A streptococci with hexadecane droplets. Journal of Bacteriology 154:139–145
    [Google Scholar]
  16. Pedersen K. 1980; Electrostatic interaction chromatography; a method for assaying the relative surface charges of bacteria. FEMS Microbiology Letters 12:365–367
    [Google Scholar]
  17. Penn C.W. 1981; Avoidance of host defences by Treponema pallidum in situ and on extraction from infected rabbit testes. Journal of General Microbiology 126:69–75
    [Google Scholar]
  18. Penn C.W. 1983; The use of the rabbit as a model for syphilis infection. In Experimental Bacterial and Parasitic InfectionsEdited by Keusch G., Wadstrom T. Amsterdam: Elsevier Biomedical;145–151
    [Google Scholar]
  19. Penn C.W., Rhodes J.G. 1982; Surface-associated antigens of Treponema pallidum concealed by an inert outer layer. Immunology 46:9–16
    [Google Scholar]
  20. Penn C.W., Cockayne A., Bailey M.J. 1985; The outer membrane of Treponema pallidum: biological significance and biochemical properties. Journal of General Microbiology 131:2349–2357
    [Google Scholar]
  21. Shanbhag V.F., Johanssen G. 1974; Specific extraction of human serum albumin by partition in aqueous biphasic systems containing polyethylene glycol bound ligand. Biochemical and Biophysical Research Communications 61:1141–1146
    [Google Scholar]
  22. Speert O., Quie P.G., Wannamaker L.W. 1981; Enhanced phagocytosis of group A streptococci M type 6 by oleic acid. Journal of Infectious. Journal of Infectious Diseases 143:570–571
    [Google Scholar]
  23. Stendahl O., Tagesson C., Edebo M. 1973; Partition of Salmonella typhimurium in a two-polymer aqueous phase system in relation to liability to phagocytosis. Infection and Immunity 8:36–41
    [Google Scholar]
  24. Stendahl O., Tagesson C., Edebo M. 1974; Influence of hyperimmune immunoglobulin G on the physicochemical properties of the surface of Salmonella typhimurium 395 MS in relation to interaction with phagocytic cells. Infection and Immunity 10:316–319
    [Google Scholar]
  25. Stevens O., Winston C., Van Dyke M. 1974; In vitro evaluation of opsonic and cellular granulocyte function by luminol-dependent chemiluminescence: utility in patients with severe neutropenia and cellular deficiency states. Infection and Immunity 22:41–51
    [Google Scholar]
  26. Stjernstrom I., Magnusson K.E., Stendahl O., Tagesson C. 1977; Liability to hydrophobic and charge interaction of smooth Salmonella typhimurium 395 MS sensitized with anti-MS immunoglobulin G and complement. Infection and Immunity 18:261–265
    [Google Scholar]
  27. Strandberg Pedersen I., Axelsen K.E., Sand Pedersen O. 1981; Antigenic analysis of Treponema pallidum: cross reactions between individual antigens of T. pallidum and T. reiter. . Scandinavian Journal of Immunology 13:143–150
    [Google Scholar]
  28. Van Oss I. 1981; APhagocytosis as a surface phenomenon. . Annual Review of Microbiology 32:19–39
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-132-1-133
Loading
/content/journal/micro/10.1099/00221287-132-1-133
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error