1887

Abstract

SUMMARY: Botulinum C toxin (C2T) is composed of two dissimilar protein components, designated components I and II, which are linked with neither covalent nor noncovalent bonds. The heterogeneity of these two components of C2T produced by type C and D strains was examined. Of 21 strains examined, 19 strains produced the two components, while the others produced neither component I nor component II. The 19 producers of C2T could be divided into three groups based on the differences in antigenicity, molecular weight and biological activity of components I and II. The results provide evidence of heterogeneity in the molecular structure of the two components of C2T, which is possibly a cause of the differences in the biological activity of the toxin observed in different strains.

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/content/journal/micro/10.1099/00221287-132-1-125
1986-01-01
2022-06-29
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References

  1. Eklund M. W., Poysky F. T. 1972; Activation of a toxic component of Clostridium botulinum types C and D by trypsin. Applied Microbiology 24108–113
    [Google Scholar]
  2. Eklund M. W., Poysky S. M., Reed S. M., Smitm C. A. 1971; Bacteriophage and the toxigenicity of Clostridium botulinum type C. Science 172408–482
    [Google Scholar]
  3. Engvall E., Perlmann P. 1971; Enzyme-linked immunosorbent assay (ELISA) quantitative assay of immunoglobulin G. Immunochemistry 8874–879
    [Google Scholar]
  4. Jansen B.C., Knoetze P.C. 1977; The taxonomic position of Clostridium botulinum type C. Onderstepoort Journal of Veterinary Research 4453–54
    [Google Scholar]
  5. Nakamura S., Serikawa T., Yamakawa K., Nishida S., Kozakai S., Sakaguchi G. 1978; Sporulation and C2 toxin production by Clostridium botulinum type C strains producing no C1 toxin. Microbiology and Immunology 22591–596
    [Google Scholar]
  6. Ohishi I. 1983; Response of mouse intestinal loop to botulinum C2 toxin : enterotoxic activity induced by cooperation of nonlinked protein components. Infection and Immunity 40691–695
    [Google Scholar]
  7. Ohishi I., Odagiri Y. 1983; Histopathological effect of botulinum C2 toxin on mouse intestine. Infection and Immunity 4354–58
    [Google Scholar]
  8. Ohishi I., Sakaguchi G. 1982; Production of C2 toxin by Clostridium botulinum types C and D as determined by its vascular permeability activity. Infection and Immunity 351–4
    [Google Scholar]
  9. Ohishi I., Iwasaki M., Sakaguchi G. 1980; Purification and characterization of two components of botulinum C2 toxin. Infection and Immunity 30668–673
    [Google Scholar]
  10. Ohishi I., Iwasaki M., Sakaguchi G. 1981; Vascular permeability activity of botulinum C2 toxin elicited by cooperation of two dissimilar protein components. Infection and Immunity 31890–895
    [Google Scholar]
  11. Ohishi I., Miyake M., Ogura K., Nakamura S. 1984; Cytopathic effect of botulinum C2 toxin on tissue-culture cells. FEMS Microbiology Letters 23281–284
    [Google Scholar]
  12. Towbin H., Staehelin T., Gordon J. 1984; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets : procedures and some applications. Proceedings of the National Academy of Sciences of the United States of America 764350–4354
    [Google Scholar]
  13. Weber K., Osborn M. 1969; The reliability of molecular weight determination by dodecyl sulfatepolyacrylamide gel electrophoresis. Journal of Biological Chemistrji 2444406–4412
    [Google Scholar]
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