1887

Abstract

Summary: Early in sporulation, cells of wild-type produce three proteases ( and ) with monomeric values of about 65000, 53000 and 43500, anda further protease, ( about 30000) at the time of coat assembly. An additional protease, ( about 15000) appears transiently in sporangia at about the time of spore release. Three strains with defective spore coats were examined for alterations in sporulation proteases. A strain carrying the ger36 mutation produces and normally, fails to produce and accumulates on or inits spores. A strain carrying the mutation produces normal quantities of proteases and , but has a reduced amount of proteases and . A strain carrying both the and the mutations accumulates neither protease nor . The wild-type allele of the gene was cloned in the vector, phage 105J9. Complementation tests with the cloned gene showed that the mutation is recessive to the wild-type allele.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-131-9-2421
1985-09-01
2021-10-20
Loading full text...

Full text loading...

/deliver/fulltext/micro/131/09/mic-131-9-2421.html?itemId=/content/journal/micro/10.1099/00221287-131-9-2421&mimeType=html&fmt=ahah

References

  1. Anagnostopoulos C., Spizizen J. 1961; Requirements for transformation in Bacillus suhtilis . Journal of Bacteriology 81:741–746
    [Google Scholar]
  2. Boschwitz H., Milner Y., Keynan A., HalvorSon H. O., Trol w. 1983; The effect of inhibitors of trypsin-like proteolytic enzymes on Bacillus cereus T spore germination. Journal of Bacteriology 153:700–708
    [Google Scholar]
  3. Bugaichuk U. D., Deadman M., Errington J., Savva D. 1984; Restriction enzyme analysis of Bacillus subtilis bacteriophage ϕ,105 DNA. Journal of General Microbiology 130:2165–2167
    [Google Scholar]
  4. Dancer B. N., Mandelstam J. 1975; Production and possible function of serine protease during sporulation of Bacillus subtilis . Journal of Bacteriology 121:406–410
    [Google Scholar]
  5. Errington J. 1984; Efficient Bacillus suhtilis cloning system using bacteriophage vector ϕI05J9. Journal of General Microbiology 130:2615–2628
    [Google Scholar]
  6. Flock J. I. 1977; Deletion mutants of the temperate Bacillus subtilis bacteriophage ϕ 105. Molecular and General Genetics 155:241–247
    [Google Scholar]
  7. Foster J. W., Perry J. J. 1954; Intracellular events occurring during endotrophic sporulation in Bacillus mycoides . Journal of Bacteriology 67:295–302
    [Google Scholar]
  8. Goldman R. C., Tipper D. J. 1978; Bacillus subtilis spore coats: complexity and purification of a unique polypeptide component. Journal of Bacteriology 135:1091–1106
    [Google Scholar]
  9. Hackett R. H., Setlow P. 1983; Enzymatic activity of precursors of Bacillus megaterium spore protease. Journal of Bacteriology 153:357–358
    [Google Scholar]
  10. Hageman J. H., Carlton B. C. 1973; Effects of mutational loss of specific intracellular proteases on the sporulation of Bacillus suhtilis . Journal of Bacteriology 114:612–617
    [Google Scholar]
  11. Heussen C., Dowdle E. B. 1980; Electrophoretic analysis of plasminogen activators in polyacrylamide gels containing sodium dodecyl sulphate and copolymerised substrates. Analytical Biochemistry 102:l96–202
    [Google Scholar]
  12. Irie R., Okamoto T., Fujita Y. 1982; A germination mutant of Bacillus subtilis deficient in response to glucose. Journal4 General and Applied Microbiology 28:345–354
    [Google Scholar]
  13. James W., Mandelstam J. 1985; spoV/C, a new sporulation locus in Bacillus subtilis affecting spore coats, germination and the rate of sporulation. Journal of General Microbiology 131:2409–2419
    [Google Scholar]
  14. Jenkinson H. F. 1983; Altered arrangement of proteins in the spore coat of a germination mutant of Bacillus subtilis . Journal of General Microbiology 129:1945–1958
    [Google Scholar]
  15. Jenkinson H. F., Lord H. 1983; Protease deficiency and its association with defects in spore coat structure. Journal of General Microbiology 129:2727–2737
    [Google Scholar]
  16. Jenkinson H.F., Kay D., Mandelstam J. 1980; Temporal dissociation of late events in Bacillus subtilis sporulation from expression of the genes that determine them. Journal of Bacteriology 141:793–805
    [Google Scholar]
  17. Jenkinson H.F., Sawyer W. D., Mandelstam J. 1981; Synthesis and order of assembly of spore coat proteins in Bacillus subtilis . Journal of General Microbiology 123:1–16
    [Google Scholar]
  18. Kobayashi Y., Hirochika H., Kawamura F., Saito H. 1981; Cloning and characterisation of sporulation genes in Bacillus subtilis . In Sporulation and Germination114–118 Edited by Levinson H., Sonenshein A. L., Tipper D. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  19. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. Journal of Molecular Biology 3:208–218
    [Google Scholar]
  20. Moir A. 1981; Germination properties of a spore coat defective mutant of Bacillus subtilis 168. Journal of Bacteriology 146:1106–1116
    [Google Scholar]
  21. Postemsky C. J., Dignam S.S., Setlow P. 1978; Isolation and characterisation of mutants of Bacillus megaterium having decreased levels of spore protease. Journal of Bacteriology 135:841–850
    [Google Scholar]
  22. Prestidge L., Gage V., Spizizen J. 1971; Protease activities during the course of sporulation in Bacillus subtilis . Journal of Bacteriology 107:185–213
    [Google Scholar]
  23. Sastry K. J., Srivastava O. P., Millet J., Aronson A. I. 1983; Characterisation of Bacillus subtilis mutants with a temperature-sensitive intra cellular protease. Journal of Bacteriology 153:511–519
    [Google Scholar]
  24. Schaeffer P., Ionesco H., Ryter A., Balassa G. 1965; La sporulation de Bacillus subtilis: étude génétique et physiologique. Colloques Internationaux de Centre National de la Recherche Scientffique, Marseille 124:553–563
    [Google Scholar]
  25. Sterlini J. M., Mandelstam J. 1969; Commitment to sporulation in Bacillus subtilis and its relationship to development of actinomycin resistance. Biochemical Journal 113:29–37
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-131-9-2421
Loading
/content/journal/micro/10.1099/00221287-131-9-2421
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error