Saccharomyces cerevisiae to Mutants Resistant β-L-Aspartylhydroxamate

Summary: Yeast strains resistant to the L-asparagine analogue β-L-aspartylhydroxamate have been shown to have mutations in any of at least three unlinked genes. Mutation in one of these genes, ahrl, is dominantly expressed and affects a function that involves nitrogen metabolism in the cells, including the exclusion of amino acids at the level of transport. The general amino acid permease is rendered sensitive to ammonium ion in strains carrying the dominantly expressed mutations in ahrl, but other functions related to nitrogen metabolism probably are involved as well.