Summary: Cell extracts of and were examined for enzymes of intermediary carbohydrate metabolism using a sensitive radiochemical assay procedure. For , the enzyme activities detected were supporting evidence for the existence of a glycolytic pathway giving lactate anaerobically and acetate aerobically. also had activities of many glycolytic enzymes. Enzymes of the pentose phosphate pathway occurred in both and . Their presence allowed the proposal of a sequence for the synthesis from glycolytic pathway intermediates of ribose 5-phosphate, and hence phospho-ribosyl diphosphate, for the synthesis of nucleotides. Pathways for the further metabolism of deoxyribose 1-phosphate and ribose 1-phosphate produced from nucleoside phosphorylase reactions operated in extracts from both organisms.


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