1887

Abstract

Summary: NCIB 7423 carries two cryptic plasmids, pCB101 (6·05 kbp) and pCB102 (7·8 kbp). Sites for the restriction enzymes RI, RV, III, I and I have been found in one or both of these plasmids and their relative positions determined. Restriction fragments from both plasmids have been inserted into a vector plasmid (pJAB1) that is able to replicate in but not in and the recombinant plasmids have been established in . A 3·3 kbp 3A fragment of pCB101 conferred upon the vector the ability to transform both Recand Recstrains of . Plasmid pRB1, a representative chimaera carrying only the 3·3 kbp 3A fragment of pCB101, was successfully transferred from back to . Plasmid pRB1 was readily lost from in the absence of selection. This evidence, together with the results of hybridization experiments, suggests that pRB1 is present as a weakly replicating autonomous element in . A recombinant plasmid carrying a 2·0 kbp 3A fragment of pCB102 underwent integration into the chromosome.

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/content/journal/micro/10.1099/00221287-131-8-2097
1985-08-01
2021-10-17
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