1887

Abstract

Summary: Flagella from , an obligately anaerobic bacterium originally isolated from murine caecal mucosa, were purified by mechanical shearing followed by differential centrifugation. Purity of the flagellar preparation was determined by polyacrylamide gel electrophoresis, electron microscopy and chemical analysis. The flagella were composed of a single protein subunit (flagellin) with an estimated molecular weight of 42000. The amino acid composition of the flagellin was similar to that of some facultatively anaerobic and aerobic bacteria.

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/content/journal/micro/10.1099/00221287-131-8-2075
1985-08-01
2024-12-03
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References

  1. Chet I., Mitchell R. 1976; Ecological aspects of microbial chemotactic behavior. Annual Review of Microbiology 30:221–239
    [Google Scholar]
  2. DeLange R. J., Chang J. Y., Shaper J. H., Glazer A. 1976; Amino acid sequence of flagellin of Bacillus subtilis 168. Journal of Biological Chemistry 251:705–711
    [Google Scholar]
  3. Dimmitt K., Simon M. 1970; Antigenic nature of bacterial hook structures. Infection and Immunity 1:212–213
    [Google Scholar]
  4. Fairbanks G., Steck T. L., Wallach D. F. H. 1971; Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane. Biochemistry 10:2606–2617
    [Google Scholar]
  5. Guffanti A. A., Eisenstein H. C. 1983; Purification and characterization of flagella from the alkalophile Bacillus firmus RAB. Journal of General Microbiology 129:3239–3242
    [Google Scholar]
  6. Horikoshi K., Akiba T. 1982 Alkalophilic Microorganisms. A New Microbial World pp 75–77 Tokyo: Japan Scientific Societies Press & Springer-Verlag;
    [Google Scholar]
  7. Iino T. 1977; Genetics of structure and function of bacterial flagella. Annual Review of Genetics 11:161–182
    [Google Scholar]
  8. Kagawa H., Owaribe K., Asakura S., Takahashi N. 1976; Flagellar hook protein from Salmonella SJ25. Journal of Bacteriology 125:68–73
    [Google Scholar]
  9. Karkhanis Y. D., Zeltner J. Z., Jackson J. J., Carlo D. J. 1978; A new and improved micro-assay to determine 2-keto-3-deoxyoctonate in lipo-polysaccharide of gram negative bacteria. Analytical Biochemistry 85:595–601
    [Google Scholar]
  10. Kondoh H., Hotani H. 1974; Flagellin from E. coli K12: polymerization and molecular weight in comparison with Salmonella flagellins. Biochimica et biophysica acta 336:117–139
    [Google Scholar]
  11. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  12. Savage D. C. 1981; The microbial flora in the gastrointestinal tract. In Nutrition in Health and Disease and International Development: Symposium from the XII International Congress of Nutrition pp 893–908 New York: Alan R. Liss;
    [Google Scholar]
  13. Smith R. W., Koffler H. 1971; Bacterial flagella. Advances in Microbial Physiology 6:219–339
    [Google Scholar]
  14. Stanton T. B., Savage D. C. 1983; Roseburia cecicola, gen. nov., sp. nov., a motile, obligately anaerobic bacterium from the mouse cecum. International Journal of Systematic Bacteriology 33:618–627
    [Google Scholar]
  15. Stanton T. B., Savage D. C. 1984; Motility as a factor in bowel colonization by Roseburia cecicola, an obligately anaerobic bacterium from the mouse caecum. Journal of General Microbiology 130:173–183
    [Google Scholar]
  16. Stocker B. A. D., Campbell J. C. 1959; The effect of non-lethal deflagellation on bacterial motility and observations on flagellar regeneration. Journal of General Microbiology 20:670–685
    [Google Scholar]
  17. Whitaker J. R., Granum P. E. 1980; An absolute method for protein determination based on differences in absorbance at 235 and 280 nm. Analytical Biochemistry 109:156–158
    [Google Scholar]
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