1887

Abstract

Summary: Representative strains of coagulase-positive and coagulase-negative staphylococci were degraded by acid methanolysis and the resultant fatty acid methyl esters analysed by gas chromatography. The quantitative data obtained were examined by cluster analysis. The coagulase-positive strains formed six major and one single-member cluster at the 90% S-level. The aggregate cluster included the single-member cluster and major clusters 1 and 2. The four remaining clusters contained strains and were homogeneous and distinct. The coagulase-negative strains were recovered in ten major and three single-member clusters at the 90% -level. Five of the ten major clusters were reasonably homogeneous with respect to the existing classification. Thus, three strains and five of the six strains, two of the three strains and five of the six strains were recovered in separate clusters. Cluster 7 was divided into two subclusters; one contained five of the six strains and the other contained the two representatives of . The remaining clusters were heterogeneous with regard to the named strains they contained.

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1985-08-01
2024-04-20
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References

  1. Alvey W. G., Banfield C. F., Baxter R. I., Gower J. C., Krzanowski W. J., Lane P. W., Leech P. K., Welder J. A., Payne R. W., Phelps K. M., Rogers C. E., Ross G. J. S., Simpson H. R., Todd A. D., Wedderburn R. W. M., Wilkinson G. N. 1977 GENSTAT Reference Manual Harpenden, UK: Rothamsted Experimental Station;
    [Google Scholar]
  2. Baird-Parker A. C. 1974; Staphylococcus. In Bergey's Manual of Determinative Bacteriology 8th edn, pp 483–489 Edited by Buchanan R. E., Gibbons N. E. Baltimore: Williams & Wilkins;
    [Google Scholar]
  3. Bøe B., Gjerde J. 1980; Fatty acid patterns in the classification of some representatives of the families Enterobacteriaceae and Vibrionaceae . Journal of General Microbiology 116:41–49
    [Google Scholar]
  4. Bousfield I. J., Smith G. L., Dando T. R., Hobbs G. 1983; Numerical analysis of total fatty acid profiles in the identification of coryneform, nocardioform and some other bacteria. Journal of General Microbiology 129:375–394
    [Google Scholar]
  5. Brun Y., Fleurette J., Forey F. 1978; Micromethod for biochemical identification of coagulase-negative staphylococci. Journal of Clinical Microbiology 8:503–508
    [Google Scholar]
  6. Doern G. V., Earls J. E., Jeznach P. A., Parker D. S. 1983; Species identification and biotyping by the API STAPH IDENT system. Journal of Clinical Microbiology 17:260–263
    [Google Scholar]
  7. Drucker D. B. 1981 Microbiological Applications of Gas-Chromatography Cambridge: University Press;
    [Google Scholar]
  8. Durham D. R., Kloos W. E. 1978; Comparative study of the total cellular fatty acids of Staphylococcus species of human origin. International Journal of Systematic Bacteriology 28:223–228
    [Google Scholar]
  9. Egge H., Murawski U., Chatranon W., Zilliken F. 1971; Die verzweigten Fettsauren von Straphylococcus aureus, eine gaschromatographisch-massenspektrometriche Analyse. Zeitschrift für Naturforschung 26b:893–901
    [Google Scholar]
  10. Feltham R. K. A., Sneath P. H. A. 1982; Construction of matrices for computer-assisted identification of aerobic Gram-positive cocci. Journal of General Microbiology 128:713–720
    [Google Scholar]
  11. Gladek A., Tkacz A., Zakrzewska J., Mordarski M., Goodfellow M., Pulverer G. 1985; Characterisation of xylose positive staphylococci by DNA–DNA reassociation. Zentralblatt für Bakteriologie und Hygiene (Abteilung I, Supplement) (in the Press)
    [Google Scholar]
  12. Goodfellow M. 1985; Staphylococcal systematics: past, present and future. Zentralblatt für Bakteriologie und Hygiene (Abteilung I, Supplement) (in the Press)
    [Google Scholar]
  13. Goodfellow M., Minnikin D. E. 1985; Introduction to chemosystematics. In Chemical Methods in Bacterial Systematics pp 1–15 Edited by Goodfellow M., Minnikin D. E. London & New York: Academic Press;
    [Google Scholar]
  14. Goodfellow M., Mordarski M., Tkacz A., Szyba K., Pulverer G. 1980; Polynucleotide sequence divergence among some coagulase-negative staphylococci. Zentralblatt für Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene (Abteilung I, Originale A) 246:10–22
    [Google Scholar]
  15. Goodfellow M., Alderson G., Nahaie M. R., Peters G., Schumacher-Perdeau F., Pulverer G., Heczko P. B., Mordarski M. 1983; Numerical taxonomy of Staphylococcus . Zentralblatt für Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene (Series A) 256:7–24
    [Google Scholar]
  16. Gower J. C. 1971; A general coefficient of similarity and some of its properties. Biometrics 27:857–874
    [Google Scholar]
  17. Hájek V. 1976; Staphylococcus intermedius, a new species isolated from animals. International Journal of Systematic Bacteriology 26:401–408
    [Google Scholar]
  18. Hájek V., Marsalek E. 1971; The differentiation of pathogenic staphylococci and a suggestion for their taxonomic classification. Zentralblatt für Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene (Abteilung I, Originale A) 217:176–182
    [Google Scholar]
  19. Hájek V., Marsalek E. 1976; Evaluation of classificatory criteria for staphylococci. Zentralblatt für Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene (Abteilung I,)Supplement 5:11–21
    [Google Scholar]
  20. Hájek V., Schindler J. 1981; Cluster analysis of coagulase-positive staphylococci. Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene (Abteilung I,)Supplement 10:27–35
    [Google Scholar]
  21. Ishizuka I., Ueta N., Yamakawa T. 1966; Gas chromatographic studies of microbial components. II. Carbohydrate and fatty acid constitution of the family Micrococcaceae . Japanese Journal of Experimental Medicine 36:73–83
    [Google Scholar]
  22. Jantzen E., Bergan T., Bøvre K. 1974; Gas chromatography of bacterial whole cell methanolysates. VI. Fatty acid composition of strains within Micrococcaceae . Acta pathologica et microbiologica scandinavica 82B:785–798
    [Google Scholar]
  23. Kloos W. E. 1980; Natural populations of the genus Staphylococcus . Annual Review of Microbiology 34:559–592
    [Google Scholar]
  24. Kloos W. E., Schleifer K. H. 1975; Simplified scheme for routine identification of human Staphylococcus species. Journal of Clinical Microbiology 1:82–87
    [Google Scholar]
  25. Kloos W. E., Schleifer K. H. 1981; The genus Staphylococcus . In The Prokaryotes: a Handbook of Habitats, Isolation and Identification of Bacteria vol 2 pp 1548–1589 Edited by Starr M. P., Stolp H., Trüper H. G., Balows A., Schlegel H. G. Berlin: Springer-Verlag;
    [Google Scholar]
  26. Kloos W. E., Wolfshohl J. S. 1982; Identification of Staphylococcus species with the API STAPHIDENT. Journal of Clinical Microbiology 16:509–516
    [Google Scholar]
  27. Komaratat P., Kates M. 1975; The lipid composition of a halotolerant species of Staphylococcus epidermidis . Biochimica et biophysica acta 398:464–484
    [Google Scholar]
  28. Kushwaha S. C., Kates M. 1976; Nonpolar lipids of a halotolerant species of Staphylococcus epidermidis . Canadian Journal of Biochemistry 54:79–85
    [Google Scholar]
  29. Macfarlane M. G. 1962; Lipid components of Staphylococcus aureus and Salmonella typhimurium . Biochemical Journal 82:40P–41P
    [Google Scholar]
  30. Meyer S. A., Schleifer K. H. 1978; Deoxyribonucleic acid reassociation in the classification of coagulase-positive staphylococci. Archives of Microbiology 117:183–188
    [Google Scholar]
  31. Minnikin D. E., Hutchinson I. G., Caldicott A. B., Goodfellow M. 1980; Thin-layer chromatography of methanolysates of mycolic acid containing bacteria. Journal of Chromatography 188:221–233
    [Google Scholar]
  32. Miwa T. K., Mikolajczak K. L., Earle F. R., Wolff I. A. 1960; Gas chromatographic characterization of fatty acids. Analytical Chemistry 32:1739–1742
    [Google Scholar]
  33. Morrison S. J., Tornabene T. G., Kloos W. E. 1971; Neutral lipids in the study of the relationships of members of the family Micrococcaceae . Journal of Bacteriology 108:353–358
    [Google Scholar]
  34. Nahaie M. R., Goodfellow M., Minnikin D. E., Hájek V. 1984; Polar lipid and isoprenoid quinone composition in the classification of Staphylococcus . Journal of General Microbiology 130:2427–2437
    [Google Scholar]
  35. O'Donnell A. G. 1985; Numerical analysis of chemotaxonomic data. In Computer Assisted Bacterial Systematics pp 403–414 Edited by Goodfellow M., Jones D., Priest F. G. London & New York: Academic Press;
    [Google Scholar]
  36. O'Donnell A. G., Goodfellow M., Minnikin D. E. 1982; Lipids in the classification of Nocardioides: reclassification of Arthrobacter simplex (Jensen) Lochhead in the genus Nocardioides (Prauser) emend. O'Donnell et al. as Nocardioides simplex comb. nov. Archives of Microbiology 133:323–329
    [Google Scholar]
  37. Rédai I., Réthy A., Váczi L. 1967; Fatty acid composition of Staphylococcus aureus strains differing in antibiotic sensitivity. Acta microbiologica Academiae scientiarum hungaricae 14:77–82
    [Google Scholar]
  38. Rédai I., Réthy A., Rozgonyi F. 1969; Fatty acids in Staphylococcus aureus and Escherichia coli cultured in vivo. Acta microbiologica Academiae scientiarum hungaricae 16:219–225
    [Google Scholar]
  39. Rédai I., Réthy A., Sebessi-Gönczy P., Váczi L. 1971; Lipids in Staphylococcus aureus and Escherichia coli cultured in the presence of human serum. Acta microbiologica Academiae scientiarum hungaricae 18:297–306
    [Google Scholar]
  40. Rédai I., Sebessi-Gönczy P., Váczi L. 1972; Effect of chloramphenicol on phospholipid synthesis in sensitive Staphylococcus aureus strains. Acta microbiologica Academiae scientiarum hungaricae 19:187–193
    [Google Scholar]
  41. Schleifer K. H., Kandler O. 1972; Peptidogly-can types of bacterial cell walls and their taxonomic implications. Bacteriological Reviews 36:407–477
    [Google Scholar]
  42. Schleifer K. H., Schumacher-Perdreau F., Götz F., Popp B., Hájek V. 1976; Chemical and biochemical studies for the differentiation of coagulase-positive staphylococci. Archives of Microbiology 110:263–270
    [Google Scholar]
  43. Schleifer K. H., Meyer S. A., Rupprecht M. 1979; Relatedness among coagulase-negative staphylococci: deoxyribonucleic acid reassociation and comparative immunological studies. Archives of Microbiology 122:93–101
    [Google Scholar]
  44. Schleifer K. H., Geyer U., Kilpper-Balz R., Devries L. A. 1983; Elevation of Staphylococcus sciuri subsp. lentus (Kloos et al.) to species status: Staphylococcus lentus (Kloos et al.) comb. nov. Systematic and Applied Microbiology 4:382–387
    [Google Scholar]
  45. Schumacher-Perdreau F., Rotering H., Pulverer G. 1983; Cell wall analysis and taxonomy of staphylococci. Zentralblatt für Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene (Series A) 256:25–38
    [Google Scholar]
  46. Sincoweay H., Miyagawa E., Kume T. 1981; Cellular fatty acid composition in staphylococci isolated from bovine milk. National Institute of Animal Health Quarterly (Japan) 21:14–20
    [Google Scholar]
  47. Sneath P. H. A. 1985; Future of numerical taxonomy. In Computer Assisted Bacterial Systematics pp 415–431 Edited by Goodfellow M., Jones D., Priest F. G. London & New York: Academic Press;
    [Google Scholar]
  48. Sneath P. H. A., Sokal R. R. 1973 Numerical Taxonomy: The Principles and Practice of Numerical Classification San Francisco: W. H. Freeman & Co;
    [Google Scholar]
  49. Sokal R. R., Michener C. D. 1958; A statistical method for evaluating systematic relationships. University of Kansas Science Bulletin 38:1409–1438
    [Google Scholar]
  50. Szulga T., Meyer W., Witte W., Horn G. 1976; Numerical taxonomy of Staphylococcus aureus . Zentralblatt für Bakteriologie (Abteilung I,) Supplement 5:101–104
    [Google Scholar]
  51. Tornabene T. G., Morrison S. J., Kloos W. E. 1970; Aliphatic hydrocarbon contents of various members of the family Micrococcaceae . Lipids 5:929–937
    [Google Scholar]
  52. Váczi L., Rédai I., Réthy A. 1967; Changes in fatty acid composition under various cultural conditions. Acta microbiologica Academiae scientiarum hungaricae 14:293–298
    [Google Scholar]
  53. Wellington E. M. H., Williams S. T. 1979; Preservation of actinomycete inoculum in frozen glycerol. Microbios Letters 6:151–157
    [Google Scholar]
  54. White D. C., Frerman F. E. 1968; Fatty acid composition of the complex lipids of Staphylococcus aureus during formation of the membrane bound electron transport system. Journal of Bacteriology 95:2198–2209
    [Google Scholar]
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