1887

Abstract

Summary: Lipopolysaccharide (LPS) from strains of pv. isolated from plum and cherry contained a uniform core region, which, in all cases, was composed of 2-keto-3-deoxyoctonic acid, heptose, glucosamine, galactosamine, rhamnose, glucose, alanine, ethanol-amine and phosphate. Lipid A contained glucosamine, phosphate and the fatty acids 3-OH 10:0, 12:0, 2-OH 12:0, and 3-OH 12:0. Sidechains of LPS from two virulent plum isolates were composed of glucose and rhamnose, and were susceptible to hydrolysis by rhamnanase from the typing phage A7, which uses LPS as its binding site. Phage A7-resistant mutants of the virulent cherry isolate C28 contained rough LPS, did not adsorb A7, and displayed reduced virulence towards cherry. An avirulent, phage A7-resistant mutant of a plum isolate contained smooth LPS with sidechains of a high glucose content that were resistant to phage hydrolysis. Loss or alteration of sidechains therefore correlated with loss of virulence.

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/content/journal/micro/10.1099/00221287-131-8-1941
1985-08-01
2019-10-21
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