1887

Abstract

Summary: Pertussis toxin (pertussigen) purified from the cytoplasmic fraction of strain 18334, phase 1, consisted of five subunits which included an additional subunit (S1a) not previously reported. Subunits S1, S1a and S2 showed extensive structural homology when analysed by one-dimensional peptide mapping, indicating that the latter two were probably derived from proteolytic cleavage of the largest subunit, S1. Subunits S3 and S4,5 generated only a limited number of peptides following chemical and enzymic degradation, but these subunits differed structurally from each other and from those showing structural homology.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-131-8-1897
1985-08-01
2022-01-17
Loading full text...

Full text loading...

/deliver/fulltext/micro/131/8/mic-131-8-1897.html?itemId=/content/journal/micro/10.1099/00221287-131-8-1897&mimeType=html&fmt=ahah

References

  1. Arai H., Sato Y. 1976; Separation and characterization of the distinct haemagglutinins contained in purified leukocytosis-promoting factor from Bordetella pertussis . Biochimica et biophysica acta 444:765–782
    [Google Scholar]
  2. Cleveland D. W., Fischer S. G., Kirschner M. W., Laemmli U. K. 1977; Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis.. Journal of Biological Chemistry 252:1102–1106
    [Google Scholar]
  3. Collier R. J. 1975; Diphtheria toxin: mode of action and structure.. Bacteriological Reviews 39:54–85
    [Google Scholar]
  4. Cowell J. L., Sato Y., Sato H., An der Lan B., Manclark C. R. 1979; Separation, purification, and properties of the filamentous haemagglutinin and the leukocytosis promoting factor-haemagglutinin from Bordetella pertussis.. In International Symposium on Pertussis, pp 371–379 Edited by Manclark C. R., Hill J. C. DHEW publication no. 79-1830. Washington, DC: United States Government Printing Office;
    [Google Scholar]
  5. Gill D. M. 1976; The arrangement of subunits in cholera toxin.. Biochemistry 15:1242–1248
    [Google Scholar]
  6. Gill D. M. 1978; Seven toxic peptides that cross cell membranes.. In Bacterial Toxins and Cell Membranes, pp 291–332 Edited by Jeljaszewicz J., Wadström T. London: Academic Press;
    [Google Scholar]
  7. Gill D. M., Clements J. D., Robertson D. C., Finkelstein R. A. 1981; Subunit number and arrangement in Escherichia coli heat-labile enterotoxin.. Infection and Immunity 33:677–682
    [Google Scholar]
  8. Imaizumi A., Suzuki Y., Ono S., Sato H., Sato Y. 1983; Effect of heptakis(2,6-O-dimethyl)-β-cyclodextrin on the production of pertussis toxin by Bordetella pertussis . Infection and Immunity 41:1138–1143
    [Google Scholar]
  9. Irons L. I., MacLennan A. P. 1979; Isolation of the lymphocytosis promoting factor-haemagglutinin of Bordetella pertussis by affinity chromatography.. Biochimica et biophysica acta 580:175–185
    [Google Scholar]
  10. Katada T., Ui M. 1977; Perfusion of the pancreas isolated from pertussis-sensitized rats: potentiation of insulin secretory responses due to β-adrenergic stimulation.. Endocrinology 101:1247–1255
    [Google Scholar]
  11. Katada T., Ui M. 1982; ADP-ribosylation of the specific membrane protein of C6 cells by islet-activation protein associated with modification of adenylate cyclase activity.. Journal of Biological Chemistry 257:7210–7216
    [Google Scholar]
  12. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4.. Nature, London 227:680–685
    [Google Scholar]
  13. Lischwe M. A., Ochs D. 1982; A new method for partial peptide mapping using N-chlorosuccinimide/urea and peptide silver staining in sodium dodecyl sulfate-polyacrylamide gels.. Analytical Biochemistry 127:453–457
    [Google Scholar]
  14. Morse S. I., Morse J. H. 1976; Isolation and properties of the leukocytosis and lymphocytosis-promoting factor of Bordetella pertussis.. Journal of Experimental Medicine 143:1483–1502
    [Google Scholar]
  15. Munoz J. J., Bergman R. K. 1968; Histamine-sensitising factors from microbial agents, with special reference to Bordetella pertussis . Bacteriological Reviews 32:103–126
    [Google Scholar]
  16. Oakley B. R., Kirsch D. R., Morris N. R. 1980; A simplified ultrasensitive silver stain for detection of proteins in polyacrylamide gels.. Analytical Biochemistry 105:361–363
    [Google Scholar]
  17. Sekura R. D., Fish F., Manclark C. R., Meade B., Zhang Y. -L. 1983; Pertussis toxin: affinity purification of a new ADP-ribosyltransferase.. Journal of Biological Chemistry 258:14647–14651
    [Google Scholar]
  18. Tamura M., Nogimori K., Murai S., Yajima M., Ito K., Katada T., Ui M. 1982; Subunit structure of islet activating protein, pertussis toxin, in conformity with the A–B model.. Biochemistry 21:5516–5522
    [Google Scholar]
  19. Wardlaw A. C., Parton R. 1983; Bordetella pertussis toxins. Pharmacology and Therapeutics 19:1–53
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-131-8-1897
Loading
/content/journal/micro/10.1099/00221287-131-8-1897
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error