1887

Abstract

Summary: The existence of energy-dependent copper influx was demonstrated in protoplasts of . Protoplasts and mycelium were tolerant of copper over the pH range 3·0 to 5·5 but sensitive at pH 6·0. Uptake of copper was approximately ten times greater at pH 6·0 than in the lower range. At pH 3·0, influx showed saturation kinetics with a half-maximal influx at an external Cu concentration of 390 μ;M and a maximum influx rate of 22 nmol h (10 cells). Saturation kinetics were not observed at pH 6·0.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-131-8-1875
1985-08-01
2021-05-11
Loading full text...

Full text loading...

/deliver/fulltext/micro/131/8/mic-131-8-1875.html?itemId=/content/journal/micro/10.1099/00221287-131-8-1875&mimeType=html&fmt=ahah

References

  1. Babich H., Stotzky G. 1981; Manganese toxicity to fungi: influence of pH. Bulletin of Environmental Contamination and Toxicology 27:474–480
    [Google Scholar]
  2. Basu S. N., Bose R. G., Bhattacharyya J. P. 1955; Some physiological studies on a copper-tolerant Penicillium species. Journal of Scientific and Industrial Research 14:46–53
    [Google Scholar]
  3. Borst-Pauwels G. W. F. H. 1981; Ion transport in yeast. Biochimica et biophysica acta 650:88–127
    [Google Scholar]
  4. Duddridge J. E., Wainwright M. 1980; Heavy metal accumulation by aquatic fungi and reduction in viability of Gammarus pulex fed Cd2+ contaminated mycelium. Water Research 14:1605–1611
    [Google Scholar]
  5. Fuhrmann G. -F., Rothstein A. 1968; The transport of Zn2+, Co2+ and Ni2+ into yeast cells. Biochimica et biophysica acta 463:325–330
    [Google Scholar]
  6. Gadd G. M., Griffiths A. J. 1980; Influence of pH on toxicity and uptake of copper in Aureobasidium pullulans . Transactions of the British Mycological Society 75:91–96
    [Google Scholar]
  7. Gadd G. M., Chudek J. A., Foster R., Reed R. H. 1984a; The osmotic responses of Penicillium ochro-chloron: changes in internal solute levels in response to copper and salt stress. Journal of General Microbiology 130:1969–1975
    [Google Scholar]
  8. Gadd G. M., Stewart A., White C., Mowll J. L. 1984b; Copper uptake by whole cells and protoplasts of a wild-type and copper resistant strain of Saccharomyces cerevisiae . FEMS Microbiology Letters 24:231–234
    [Google Scholar]
  9. Hamlyn P. F., Bradshaw R. E., Mellon F. M., Santiago C. M., Wilson J. M., Peberdy J. F. 1981; Efficient protoplast isolation from fungi using commercial enzymes. Enzyme and Microbial Technology 3:321–325
    [Google Scholar]
  10. Mohan P. M., Rudra M. P. P., Sastry K. S. 1984; Nickel transport in nickel-resistant strains of Neurospora crassa . Current Microbiology 10:125–128
    [Google Scholar]
  11. Mowll J. L., Gadd G. M. 1983; Zinc uptake and toxicity in the yeasts Sporobolomyces roseus and Saccharomyces cerevisiae . Journal of General Microbiology 129:3421–3425
    [Google Scholar]
  12. Mowll J. L., Gadd G. M. 1984; Cadmium uptake by Aureobasidium pullulans . Journal of General Microbiology 130:279–284
    [Google Scholar]
  13. Norris P. R., Kelly D. P. 1977; Accumulation of cadmium and cobalt by Saccharomyces cerevisiae . Journal of General Microbiology 99:317–324
    [Google Scholar]
  14. Okamoto K., Fuwa K. 1974; Copper tolerance of a new strain of Penicillium ochro-chloron . Agricultural and Biological Chemistry 38:1405–1406
    [Google Scholar]
  15. Okamoto K., Suzuki M., Fukami M., Toda S., Fuwa K. 1977; Uptake of heavy metals by a copper-tolerant fungus, Penicillium ochro-chloron . Agricultural and Biological Chemistry 41:17–22
    [Google Scholar]
  16. Paton W. H. N., Budd K. 1972; Zinc uptake in Neocosmospora vasinfecta . Journal of General Microbiology 12:173–184
    [Google Scholar]
  17. Starkey R. L. 1973; Effect of pH on toxicity of copper to Scytalidium sp., a copper-tolerant fungus, and some other fungi. Journal of General Microbiology 78:217–225
    [Google Scholar]
  18. Stokes P. M., Lindsay J. E. 1979; Copper tolerance and accumulation in Penicillium ochro-chloron isolated from copper-plating solution. Mycologia 71:796–806
    [Google Scholar]
  19. Venkateswerlu G., Sastry K. S. 1970; The mechanism of uptake of cobalt ions by Neurospora crassa . Biochemical Journal 118:497–503
    [Google Scholar]
  20. White C., Gadd G. M. 1984; Isolation and purification of protoplasts from yeast-like cells, hyphae and chlamydospores of Aureobasidium pullulans . Journal of General Microbiology 130:1031–1034
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-131-8-1875
Loading
/content/journal/micro/10.1099/00221287-131-8-1875
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error