Isolation of and DNA Fragments Capable of Autonomous Replication in Yeast Free

Abstract

Summary: DNA fragments from the unicellular red algae and were inserted at various sites of plasmid pLG4 (pBR325 + a III fragment bearing the yeast 4 gene) with the purpose of isolating sequences supporting autonomous replication of plasmids in . Plasmid pools were prepared in then used to transform the 4 yeast strain X3656-7 D to prototrophy. The presence of free plasmids in the yeast transformants was demonstrated by Southern blotting hybridization between yeast DNA and P-labelled pBR325 and by the transformation of with DNA from yeast transformants. Hybrid plasmids recovered from Argbacterial transformants transformed yeast at high frequency. They contained fragments of total DNA, fragments of satellite DNA and one HI fragment of main DNA. These new plasmids have unique restriction sites which make them convenient vectors for cloning in yeast and possibly in algae and other plants.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-131-7-1745
1985-07-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/micro/131/7/mic-131-7-1745.html?itemId=/content/journal/micro/10.1099/00221287-131-7-1745&mimeType=html&fmt=ahah

References

  1. Allen M. B. 1959; Studies with Cyanidium caldarium, an anomously pigmented chlorophyte. Archiv für Mikrobiologie 32:270–277
    [Google Scholar]
  2. Ascione R., Soutwick W., Fresco J. R. 1966; Laboratory culturing of a thermophilic alga at high temperature. Science 153:752–755
    [Google Scholar]
  3. Barnes K. L., Craigie R. A., Cattini P. A., Cavalier-Smith T. 1982; Chromatin from the unicellular red alga Porphyridium has a nucleosome structure. Journal of Cell Science 57:151–160
    [Google Scholar]
  4. Belly R. T., Tansey M. R., Brock T. D. 1973; Algal excretion of 14C-labeled compounds and microbial interactions in Cyanidium caldarium mats. Journal of Phycology 9:123–127
    [Google Scholar]
  5. Bolivar F. 1978; Construction and characterization of new cloning vehicles. III. Derivatives of plasmid pBR322 carrying unique EcoRI sites for selection of EcoRI generated recombinant DNA molecules. Gene 4:121–136
    [Google Scholar]
  6. Chapman D. J. 1974; Taxonomic status of Cyanidium caldarium. The Porphyridiales and Gonotrichiales. Nova Hedwigia 25:673–682
    [Google Scholar]
  7. Charles D. 1977; Isolation and characterization of DNA from unicellular algae. Plant Science Letters 8:35–44
    [Google Scholar]
  8. Clarke L., Carbon J. 1978; Functional expression of cloned yeast DNA in Escherichia coli: specific complementation of argininosuccinate lyase (argH) mutations. Journal of Molecular Biology 120:517–532
    [Google Scholar]
  9. Crabeel M., Messenguy F., Lacroute F., Glansdorff N. 1981; Cloning arg3, the gene for ornithine carbamoyl transferase from Saccharomyces cerevisiae: expression in Escherichia coli requires secondary mutations; production of plasmid β-lactamase in yeast. Proceedings of the National Academy of Sciences of the United States of America 78:5026–5030
    [Google Scholar]
  10. Darby. G. K., Jones A. S., Kennedy J. F., Walker R. T. 1970; Isolation and analysis of the nucleic acids and polysaccharides from Clostridium welchii . Journal of Bacteriology 103:159–165
    [Google Scholar]
  11. Dubois M., Gilles K. A., Hamilton J. K., Rebers P. A., Smith F. 1956; Colorimetric method for determination of sugars and related substances. Analytical Chemistry 28:350–356
    [Google Scholar]
  12. Glineur C., Burny A. 1984; Isolation of sequences from human chromosomal DNA with ARS function in yeast and in rat cells. Archives Internationales de physiologie et de biochimie 92:B136–137
    [Google Scholar]
  13. Gudin C., Thomas D. 1981; Production de polysaccharides sulfatés par un biophotoréacteur à cellules immobilisées de Porphyridium cruentum . Comptes rendus de l’Académie des sciences de Paris 293:35–37
    [Google Scholar]
  14. Hutner S. H., Provasoli A., Schatz A., Haskins C. P. 1950; Some approaches to the study of the role of metals in the metabolism of microorganisms. Proceedings of the American Philosophical Society 94:152–170
    [Google Scholar]
  15. Jones R. F., Speer H. L., Kury W. 1963; Studies of the growth of the red alga Porphyridium cruentum . Physiologia plantarum 16:636–643
    [Google Scholar]
  16. Loppes R., Denis C. 1983; Chloroplast and nuclear DNA fragments from Chlamydomonas promoting high frequency transformation of yeast. Current Genetics 7:473–480
    [Google Scholar]
  17. Overbeeke N., Haring M. A., John H., Nijkamp J., Kool Ad. J. 1984; Cloning of Petunia hybrida chloroplast DNA sequences capable of autonomous replication in yeast. Plant Molecular Biology 3:235–241
    [Google Scholar]
  18. Pircival E., Foyle R. A. J. 1979; The extracellular polysaccharides of Porphyridium cruentum and Porphyridium aerugineum . Carbohydrate Research 72:165–176
    [Google Scholar]
  19. Rochaix J. D., van Dillewijn J. 1982; Transformation of the green alga Chlamydomonas reinhardii with yeast DNA. Nature, London 296:70–72
    [Google Scholar]
  20. Rochaix J. D., van Dillewijn J., Rahire M. 1984; Construction and characterization of autonomously replicating plasmids in the green unicellular alga Chlamydomonas reinhardii . Cell 36:925–931
    [Google Scholar]
  21. Seckback J., Fredrick J. F. 1981; On the origin of photosynthetic eukaryotic cells: Cyanidium caldarium as a “bridge” alga between prokaryotic cyanobacteria and eukaryotic rhodophytes: evidence from environmental, polysaccharide biochemistry and ultrastructural studies. In Origin of Life pp 567–574 Edited by Wolman Y. Dordrecht, The Netherlands: D. Reidel Publishing Co;
    [Google Scholar]
  22. Stinchcomb D. T., Thomas M., Kelly J., Selker E., Davis R. W. 1980; Eukaryotic DNA segments capable of autonomous replication in yeast. Proceedings of the National Academy of Sciences of the United States of America 77:4559–4563
    [Google Scholar]
  23. Uchimiya U., Ohtani T., Ohgawava T., Harada T., Sugita M., Sugiura M. 1983; Molecular cloning of tobacco chromosomal and chloroplast DNA segments capable of replication in yeast. Molecular and General Genetics 192:1–4
    [Google Scholar]
  24. Vallet J. M., Rahire M., Rochaix J. D. 1984; Localization and sequence analysis of chloroplast DNA sequences of Chlamydomonas reinhardii that promote autonomous replication in yeast. EMBO Journal 3:415–421
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-131-7-1745
Loading
/content/journal/micro/10.1099/00221287-131-7-1745
Loading

Data & Media loading...

Most cited Most Cited RSS feed