1887

Abstract

Summary: Interactions between group 1 acholeplasmaviruses and their host cells were studied. Acutely infected, chronically infected and uninfected cultures of strain JA1 were compared by their growth in broth and on agar, by the sensitivities of the uninfected and chronically infected cells to representatives of each of the three groups of acholeplasmaviruses, and by their SDS-PAGE polypeptide profiles. Acutely infected cells resembled uninfected cells by these criteria, except for the fact that progeny virus was being released. Two types of chronically infected cells were found: rapid growers (the same doubling time as uninfected cells) and slow growers. The latter resembled uninfected cells, except for their slower growth and low-level release of virus, and the former was resistant to group 1 viruses and had a unique polypeptide profile. These biological characterizations help to establish the non-lytic, non-cytocidal cycle of the group 1 acholeplasmaviruses.

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1985-07-01
2021-07-27
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References

  1. Clark K. D., Kaplan H., Ritter B., Liss A. 1985; Acholeplasma laidlawii colonial morphology variants: preliminary characterization. Annals of the New York Academy of Sciences 435:585–587
    [Google Scholar]
  2. Congdon A. L., Boatman E. S., Kenny G. E. 1979; Mycoplasmatales virus MV-M1: discovery in Acholeplasma modicum and preliminary characterization. Current Microbiology 3:111–115
    [Google Scholar]
  3. Denhardt D. T. 1978; Overview: a comparison of the isometric and filamentous phages. In The Single-Stranded DNA Phages pp 645–655 Edited by Denhardt D. T., Dressler D. H., Ray D. S. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  4. Dybvig K., Maniloff J. 1983; Integration and lysogeny by an enveloped mycoplasma virus. Journal of General Virology 64:1781–1785
    [Google Scholar]
  5. Gourlay R. N. 1971; Mycoplasmatales virus–laidlawii 2, a new virus isolated from Acholeplasma laidlawii . Journal of General Virology 12:65–67
    [Google Scholar]
  6. Hsu U. C. 1968; Propagation or elimination of viral infection in carrier cells. Bacteriological Reviews 32:387–399
    [Google Scholar]
  7. Liss A. 1977; Acholeplasma laidlawii infection by group 3 mycoplasma-virus. Virology 77:433–436
    [Google Scholar]
  8. Liss A. 1981; Release of a group 1 mycoplasma virus from Acholeplasma laidlawii after treatment with mitomycin C. Journal of Virology 40:285–288
    [Google Scholar]
  9. Liss A., Heiland R. A. 1982; Characterization of the enveloped plasmavirus MVL2 after propagation on three Acholeplasma laidlawii hosts. Archives of Virology 75:123–129
    [Google Scholar]
  10. Liss A., Heiland R. A. 1983; Colonial opacity variation in Mycoplasma pulmonis . Infection and Immunity 43:1245–1251
    [Google Scholar]
  11. Liss A., Maniloff J. 1971; Isolation of mycoplasmatales viruses and characterization of MVL1, MVL52, and MVG51. Science 173:725–727
    [Google Scholar]
  12. Liss A., Maniloff J. 1973; Infection of Acholeplasma laidlawii by MVL51 virus. Virology 55:118–126
    [Google Scholar]
  13. Liss A., Hakkarainen K., Jansson E. 1985; Acholeplasma laidlawii retains sensitivity to exogenous virus while releasing endogenous, mitomycin C induced, virus. Archives of Virology (in the Press)
    [Google Scholar]
  14. Maniloff J. 1969; Use of blood agar plates to study mycoplasma physiology. Microbios 1:125–135
    [Google Scholar]
  15. Maniloff J., Liss A. 1973; The molecular biology of mycoplasma viruses. Annals of the New York Academy of Sciences 225:149–158
    [Google Scholar]
  16. Maniloff J., Das J., Nowak J. A. 1978; Single-stranded DNA mycoplasmaviruses. In The Single-Stranded DNA Phages pp 177–184 Edited by Denhardt D. T., Dressler D. H., Ray D. S. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  17. Maniloff J., Das J., Putzrath R. M., Nowak J. A. 1979; Mycoplasma and spiroplasma viruses. In The Mycoplasmas vol I pp 411–430 Edited by Barile M. F., Razin S. New York: Academic Press;
    [Google Scholar]
  18. Maniloff J., Haberer K., Gourlay R. N., Das J., Cole R. 1982; Mycoplasma viruses. Intervirology 18:177–188
    [Google Scholar]
  19. Matthews R. E. F. 1979; Classification and nomenclature of viruses. Intervirology 12:129–296
    [Google Scholar]
  20. Milne R. G., Thompson G. W., Taylor-Robinson D. 1972; Electron microscopic observations of Acholeplasma laidlawii viruses. Archives of Virology 37:378–385
    [Google Scholar]
  21. Putzrath R. M., Maniloff J. 1977; Growth of an enveloped mycoplasmavirus and establishment of a carrier state. Journal of Virology 22:308–314
    [Google Scholar]
  22. Roger A. 1977; Study of the initial parasitic relationship between a group L1 of Gourlay Virus and a cell population of Acholeplasma laidlawii . Canadian Journal of Microbiology 23:224–229
    [Google Scholar]
  23. Roger A. 1982; Simultaneous isolation of infected and non-infected clones of mycoplasmas from plaques obtained on Acholeplasma laidlawii lawns with a virus of group L1 of Gourlay. Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene (Originale A) 252:129–131
    [Google Scholar]
  24. Roger A. 1983; Spontaneous dissociation between virus and host in Acholeplasma laidlawii lines initially infected by mycoplasmaviruses of group L1 of Gourlay. Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene (Originate A) 254:139–145
    [Google Scholar]
  25. Steinick L. E., Wieslander A., Johansson K. -E., Liss A. 1980; Membrane composition and virus susceptibility of Acholeplasma laidlawii . Journal of Bacteriology 143:1200–1207
    [Google Scholar]
  26. Working Group on the FAO/WHO Programme on Comparative Mycoplasmology 1976 The Growth Inhibition Test Document UPH/MIC/76.7. Geneva: World Health Organization.;
    [Google Scholar]
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