1887

Abstract

Summary: DNA from ATCC 12475 was partially digested with 3A and fragments were ligated into /II-cleaved pIJ702. When the ligation mixture was used to transform protoplasts of TK54, two transformants resistant to both thiostrepton and streptomycin were isolated. The hybrid plasmids pBV3 and pBV4 which they contained, carrying inserts of sizes 4·45 and 11·55 kbp respectively, each retransformed to streptomycin resistance at high efficiency. Both plasmids hybridized to restriction digests of chromosomal DNA in Southern blot experiments. deletion and sub-cloning experiments showed the sequence conferring streptomycin resistance to lie within a segment of 1·95 kbp. Extracts of TK54(pBV3) and TK54(pBV4) contained a streptomycin phosphotransferase similar to that in extracts of . Streptomycin phosphotransferase activity appeared in extracts of , TK54(pBV3) and TK54(pBV4) within 2 d of inoculation. When pBV3 and pBV4 were retransformed into with selection for thiostrepton resistance, plasmid DNA of sizes corresponding to the incoming plasmids was found in the transformants. In these transformants the phosphotransferase appeared at 1·5 rather than 2 d, and reached a level over twice that of the original strain.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-131-7-1657
1985-07-01
2021-05-18
Loading full text...

Full text loading...

/deliver/fulltext/micro/131/7/mic-131-7-1657.html?itemId=/content/journal/micro/10.1099/00221287-131-7-1657&mimeType=html&fmt=ahah

References

  1. Bolivar F., Backman K. 1979; Plasmids of Escherichia coli as cloning vectors. Methods in Enzymology 68:245–267
    [Google Scholar]
  2. Bradford M. M. 1976; A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein–dye binding. Analytical Biochemistry 72:248–254
    [Google Scholar]
  3. Buchanan R. E., Gibbons N. E. (editors) 1974 Bergey's Manual of Determinative Bacteriology 8th edn Baltimore: Williams & Wilkins;
    [Google Scholar]
  4. Chater K. F., Hopwood D. A., Kieser T., Thompson C. J. 1982; Gene cloning in Streptomyces. Current Topics in Microbiology and Immunology 96:69–95
    [Google Scholar]
  5. Cundliffe E. 1984; Self defence in antibiotic-producing organisms. British Medical Bulletin 40:61–67
    [Google Scholar]
  6. Feitelson J. S., Hopwood D. A. 1983; Cloning of a Streptomyces gene for an O-methyltransferase involved in antibiotic synthesis. Molecular and General Genetics 190:394–398
    [Google Scholar]
  7. Gil J. A., Hopwood D. A. 1983; Cloning and expression of a p-aminobenzoic acid synthetase gene of the candicidin producer, Streptomyces griseus . Gene 25:119–132
    [Google Scholar]
  8. Hara O., Beppu T. 1982; Mutants blocked in streptomycin production in Streptomyces griseus – the role of A-factor. Journal of Antibiotics 35:349–358
    [Google Scholar]
  9. Hintermann G. 1983 Aspects of the genomic organisation in Streptomyces glaucescens Doctorate of Natural Sciences thesis Swiss Federal Institute of Technology; Zürich:
    [Google Scholar]
  10. Hintermann G., Crameri R., Vögtli M., Hütter R. 1984; Streptomycin-sensitivity in Streptomyces glaucescens is due to deletions comprising the structural gene coding for a specific phosphotransferase. Molecular and General Genetics 196:513–520
    [Google Scholar]
  11. Hoopes B. C., McClure W. R. 1981; Studies on the selectivity of DNA precipitation by spermine. Nucleic Acids Research 9:5493–5504
    [Google Scholar]
  12. Hopwood D. A. 1967; Genetic analysis and genome structure in Steptomyces coelicolor . Bacteriological Reviews 31:373–404
    [Google Scholar]
  13. Hopwood D. A., Kieser T., Wright H. M., Bibb M. J. 1983; Plasmids, recombination and chromosome mapping in Streptomyces lividans 66. Journal of General Microbiology 129:2257–2269
    [Google Scholar]
  14. Horinouchi S., Hara O., Beppu T. 1983; Cloning of a pleiotropic gene that positively controls biosynthesis of A-factor, actinorhodin, and prodigiosin in Streptomyces coelicolor A3(2) and Streptomyces lividans . Journal of Bacteriology 155:1238–1248
    [Google Scholar]
  15. Horinouchi S., Kumada Y., Beppu T. 1984; Unstable genetic determinant of A-factor biosynthesis in streptomycin-producing organisms: cloning and characterization. Journal of Bacteriology 158:481–487
    [Google Scholar]
  16. Katz E., Thompson C. J., Hopwood D. A. 1983; Cloning and expression of the tyrosinase gene from Streptomyces antibioticus in Streptomyces lividans . Journal of General Microbiology 129:2703–2714
    [Google Scholar]
  17. Khokhlov A. S., Tovarova I. I., Borisova L. N., Pliner S. A., Shevchenko L. A., Kornitskaya E. Y., Ivkina N. S., Rapoport I. A. 1967; A-factor responsible for the biosynthesis of streptomycin by a mutant strain of Actinomyces streptomycini . Doklady Akademii nauk SSSR 177:232–235
    [Google Scholar]
  18. Kleiner E. M., Pliner S. A., Soifer V. S., Onoprienko V. V., Balashova T. A., Rozynov B. V., Khokhlov A. S. 1976; Structure of the A-factor, a bioregulator from Streptomyces griseus . Bioorganika Khimya 2:1142–1147
    [Google Scholar]
  19. McDonnell M. W., Simon M. N., Studier F. W. 1977; Analyses of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels. Journal of Molecular Biology 110:119–146
    [Google Scholar]
  20. Malpartida F., Hopwood D. A. 1984; Molecular cloning of the whole biosynthetic pathway of a Streptomyces antibiotic and its expression in a heterologous host. Nature, London 309:462–464
    [Google Scholar]
  21. Malpartida F., Zalacaín M., Jiménez A., Davies J. 1983; Molecular cloning and expression in Streptomyces lividans of a hygromycin B phosphotransferase gene from Streptomyces hygroscopicus . Biochemical and Biophysical Research Communications 117:6–12
    [Google Scholar]
  22. Martín J. F., Demain A. L. 1980; Control of antibiotic biosynthesis. Microbiological Reviews 44:230–251
    [Google Scholar]
  23. Martín J. F., Gil J. A. 1984; Cloning and expression of antibiotic production genes. Bio/technology 2:63–72
    [Google Scholar]
  24. Miller A. L., Walker J. B. 1969; Enzymatic phosphorylation of streptomycin by extracts of streptomycin-producing strains of Streptomyces . Journal of Bactriology 99:401–405
    [Google Scholar]
  25. Murakami T., Nojiri C., Toyama H., Hayashi E., Katumata K., Anzai H., Matsuhashi Y., Yamada Y., Nagaoka K. 1983; Cloning of antibiotic-resistance genes in Streptomyces . Journal of Antibiotics 36:1305–1311
    [Google Scholar]
  26. Nakano M. M., Mashiko H., Ogawara H. 1984; Cloning of the kanamycin resistance gene from a kanamycin-producing Streptomyces species. Journal of Bacteriology 157:79–83
    [Google Scholar]
  27. Nimi O., Ito G., Sueda S., Nomi R. 1971a; Phosphorylation of streptomycin at C6-OH of streptidine moiety by an intracellular enzyme of Streptomyces griseus . Agricultural and Biological Chemistry 35:848–855
    [Google Scholar]
  28. Nimi O., Ito G., Ohata Y., Funayama S., Nomi R. 1971b; Streptomycin phosphorylating enzymes produced by Streptomyces griseus . Agricultural and Biological Chemistry 35:856–861
    [Google Scholar]
  29. Ono H., Crameri R., Hintermann G., Hütter R. 1983; Hydroxystreptomycin production and resistance in Streptomyces glaucescens . Journal of General Microbiology 129:529–537
    [Google Scholar]
  30. Phillips I., Shannon K. 1984; Aminoglycoside resistance. British Medical Bulletin 40:28–35
    [Google Scholar]
  31. Rhodes P. M., Hunter I. S., Friend E. J., Walker M. 1984; Recombinant DNA methods for the oxytetracycline producer Streptomyces rimosus . Biochemical Society Transactions 12:586–587
    [Google Scholar]
  32. Rigby P. W. J., Dieckmann M., Rhodes C., Berg P. 1977; Labelling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. Journal of Molecular Biology 113:237–251
    [Google Scholar]
  33. Shirahama T., Furumai T., Okanishi M. 1981; A modified regeneration method for streptomycete protoplasts. Agricultural and Biological Chemistry 45:1271–1273
    [Google Scholar]
  34. Southern E. M. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98:503–517
    [Google Scholar]
  35. Sugiyama M., Mochizuki H., Nimi O., Nomi R. 1981a; Roles of streptomycin 6-kinase and ribosomal affinity to streptomycin in self protection of streptomycin producer. Biotechnology Letters 7:357–362
    [Google Scholar]
  36. Sugiyama M., Mochizuki H., Nimi O., Nomi R. 1981b; Mechanisms of protection of protein synthesis against streptomycin inhibition in a producing strain. Journal of Antibiotics 34:1183–1188
    [Google Scholar]
  37. Sugiyama M., Mochizuki H., Nimi O., Nomi R. 1982; Assessment of competitive action of streptomycin 6-kinase and streptomycin 6-phosphate in the in vitro protein synthesis of a streptomycin-producing microorganism. FEBS Letters 139:331–333
    [Google Scholar]
  38. Thompson C. J., Ward J. M., Hopwood D. A. 1980; DNA cloning in Streptomyces: resistance genes from antibiotic-producing species. Nature, London 286:525–527
    [Google Scholar]
  39. Tohyama H., Shigyo T., Okami Y. 1984; Cloning of a streptomycin resistance gene from a streptomycin producing streptomycete. Journal of Antibiotics 37:1736–1737
    [Google Scholar]
  40. Walker J. B. 1975; Pathways of biosynthesis of the guanidinated inositol moieties of streptomycin and bluensomycin. Methods in Enzymology 43:429–470
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-131-7-1657
Loading
/content/journal/micro/10.1099/00221287-131-7-1657
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error