1887

Abstract

Summary: The mutation in led to a substantial increase of the acriflavine-binding capacity of the cell, whereas the related mutations () and did not. Metal ions such as Na, K, Mg, Caand Aleffectively released the bound acriflavine, in proportion to their ionic strengths. The presence of cations, in fact, increased the survival fraction of the cells in the acriflavine-containing medium. Polymyxin B, an antibiotic which binds to membrane phospholipid, competed with acriflavine for binding sites. Cell wall digestion by treatment with lysozyme and EDTA slightly decreased the acriflavine-binding capacity. Almost no difference was observed in acriflavine-binding capacity between intact cells and cells from which lipopolysaccharide has been extracted (46·9% removed from the cells and 47·4% from the cells). Acriflavine bound to the cells was most effectively extracted by ethanol containing 1% HCl or by 2% (w/v) SDS. The difference in the acriflavine-binding capacity between the and cells was also observed in the spheroplasts. These facts indicate a relationship between the gene product and the acriflavine-binding capacity of the cells.

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/content/journal/micro/10.1099/00221287-131-7-1639
1985-07-01
2021-07-24
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