1887

Abstract

Summary: In addition to an excreted phosphatase, produces a distinctly different alkaline phosphatase which remains associated with the cells. It is the only major phosphatase contained in the cells. Little enzyme activity is found in whole cells using the standard assay with -nitrophenyl phosphate as the substrate, but maximum activity can be obtained after toluene or detergent treatment, or mechanical disruption of the cells. The phosphatase is not released from the cells by osmotic shock, or by treatment with MgCl or high LiCl concentrations. It is associated with the particulate fraction in cell-free extracts and detergents solubilize the enzyme from intact and broken cells. Separation of the inner and outer membranes of the cells by sucrose gradient centrifugation showed that most, if not all, of this phosphatase is located in the outer membrane.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-131-7-1611
1985-07-01
2021-07-27
Loading full text...

Full text loading...

/deliver/fulltext/micro/131/7/mic-131-7-1611.html?itemId=/content/journal/micro/10.1099/00221287-131-7-1611&mimeType=html&fmt=ahah

References

  1. Cheng K. -J., Ingram J. M., Costerston J. W. 1970a; Release of alkaline phosphatase from cells of Pseudomonas aeruginosa by manipulation of cation concentration and of pH. Journal of Bacteriology 104:748–753
    [Google Scholar]
  2. Cheng K. -J., Ingram J. M., Costerston J. W. 1970b; Alkaline phosphatase localization and sphaeroplast formation of Pseudomonas aeruginosa . Canadian Journal of Microbiology 16:1319–1324
    [Google Scholar]
  3. Christensen P., Cook F. D. 1978; Lysobacter, a new genus of non-fruiting gliding bacteria with a high base ratio. International Journal of Systematic Bacteriology 28:367–393
    [Google Scholar]
  4. DeMoss R. D. 1955; Glucose-6-phosphate and 6-phosphogluconic dehydrogenase from Leuconostoc mesenteroides . Methods in Enzymology 1:328–334
    [Google Scholar]
  5. Fairbanks G., Steck T. L., Wallach D. F. H. 1971; Electrophoretic analysis of major polypeptides of the human erythrocytic membrane. Biochemistry 10:2606–2616
    [Google Scholar]
  6. Hancock R. E. W., Nikaido H. 1978; Outer membranes of gram-negative bacteria, XIX. Isolation from Pseudomonas aeruginosa PAO1 and use in reconstitution and definition of the permeability barrier. Journal of Bacteriology 136:381–390
    [Google Scholar]
  7. Ingram J. M., Cheng K. -J., Costerston J. W. 1973; Alkaline phosphatase of Pseudomonas aeruginosa: the mechanism of secretion and release of the enzyme from whole cells. Canadian Journal of Microbiology 19:1407–1415
    [Google Scholar]
  8. Law J. H., Slepecky R. A. 1961; Assay of poly-β-hydroxybutyric acid. Journal of Bacteriology 82:33–36
    [Google Scholar]
  9. Lugtenberg B., Van Alphen L. 1983; Molecular architecture and functioning of the outer membrane of Escherichia coli and other gram-negative bacteria. Biochimica et biophysica acta 737:51–115
    [Google Scholar]
  10. Maizel J. V. Jr 1971; Polyacrylamide gel electrophoresis of viral proteins. Methods in Virology 5:179–246
    [Google Scholar]
  11. Neu H. C., Heppel L. A. 1965; The release of enzymes from Escherichia coli by osmotic shock and during the formation of spheroplasts. Journal of Biological Chemistry 240:3685–3692
    [Google Scholar]
  12. Orndorff P. E., Dworkin M. 1980; Separation and properties of the cytoplasmic and outer membranes of vegetative cells of Myxococcus xanthus . Journal of Bacteriology 141:914–927
    [Google Scholar]
  13. Osborn M. J., Wu H. C. P. 1980; Proteins of the outer membrane of gram-negative bacteria. Annual Review of Microbiology 34:369–422
    [Google Scholar]
  14. Osborn M. J., Gander J. E., Parisi E., Carson J. 1972; Mechanism of assembly of the outer membrane of Salmonella typhimurium . Journal of Biological Chemistry 247:3962–3972
    [Google Scholar]
  15. Poirier T. P., Holt S. C. 1983a; Acid and alkaline phosphatases of Capnocytophaga species. I. Production and cytological localization of the enzymes. Canadian Journal of Microbiology 29:1350–1360
    [Google Scholar]
  16. Poirier T. P., Holt S. C. 1983b; Acid and alkaline phosphatases of Capnocytophaga species. III. The relationship of the enzymes to the cell wall. Canadian Journal of Microbiology 29:1369–1381
    [Google Scholar]
  17. Poole K., Hancock R. E. W. 1983; Secretion of alkaline phosphatase and phospholipase C in Pseudomonas aeruginosa is specific and does not involve an increase in outer membrane permeability. FEMS Microbiology Letters 16:25–29
    [Google Scholar]
  18. Pooley H. M., Porres-Juan J. M., Shockman G. D. 1970; Dissociation of an autolytic enzyme–cell wall complex by treatment with unusually high concentration of salt. Biochemical and Biophysical Research Communications 38:1134–1140
    [Google Scholar]
  19. Reichenbach H. 1981; Taxonomy of the gliding bacteria. Annual Review of Microbiology 35:339–364
    [Google Scholar]
  20. Reichenbach H., Dworkin M. 1981; The order Cytophagales (with addenda on the genera Herpetosiphon, Saprospira, and Flexithrix). In The Prokaryotes pp 356–379 Edited by Starr M. P., Stolp H., Truper H. G., Balows A., Schlegel H. G. New York: Springer Verlag;
    [Google Scholar]
  21. Silhavy T. J., Benson S. A., Emr S. D. 1983; Mechanism of protein localization. Microbiological Reviews 47:313–344
    [Google Scholar]
  22. von Tigerstrom R. G. 1980; Extracellular nucleases of Lysobacter enzymogenes: production of the enzymes and purification and characterization of an endonuclease. Canadian Journal of Microbiology 26:1029–1037
    [Google Scholar]
  23. von Tigerstrom R. G. 1981; Extracellular nucleases of Lysobacter enzymogenes: purification and characterization of a ribonuclease. Canadian Journal of Microbiology 27:1080–1086
    [Google Scholar]
  24. von Tigerstrom R. G. 1983; The effect of magnesium and manganese ion concentrations and medium composition on the production of extracellular enzymes by Lysobacter enzymogenes . Journal of General Microbiology 129:2293–2299
    [Google Scholar]
  25. von Tigerstrom R. G. 1984; Production of two phosphatases by Lysobacter enzymogenes and purification and characterization of the extracellular enzyme. Applied and Environmental Microbiology 47:693–698
    [Google Scholar]
  26. Voelz H., Ortigoza R. O. 1968; Cytochemistry of phosphatase in Myxococcus xanthus . Journal of Bacteriology 96:1357–1365
    [Google Scholar]
  27. Whitaker D. R. 1970; The α-lytic protease of a myxobacterium. Methods in Enzymology 19:599–613
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-131-7-1611
Loading
/content/journal/micro/10.1099/00221287-131-7-1611
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error