1887

Abstract

A method of transposon mutagenesis using Tn has been developed for the facultative methylotroph AM1 using the IncP-10 plasmid R91-5. Auxotrophic mutants and mutants involving the metabolism of methanol have been isolated.

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1985-06-01
2022-01-18
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References

  1. Bachmann B. J. 1983; Linkage map of Escherichia coli K12. , 7. Microbiological Reviews 47:180–230
    [Google Scholar]
  2. Berg D. E., Berg C. M. 1983; The prokaryotic transposable element Tn5 . Bio/Technology 1:417–435
    [Google Scholar]
  3. Chandler P. M., Krishnapillai V. 1974; Phenotypic properties of R factors of Pseudomonas aeruginosa: R factors transferable only in Pseudomonas aeruginosa . Genetical Research 23:251–257
    [Google Scholar]
  4. Chandler P. M., Krishnapillai V. 1977; Characterization of Pseudomonas aeruginosa de-repressed R-plasmids. Journal of Bacteriology 130:596–603
    [Google Scholar]
  5. Dean H. F., Morgan A. F. 1983; Integration of R91-5::Tn501 into the Pseudomonas putida PPN chromosome and genetic circularity of the chromosomal map. Journal of Bacteriology 153:485–497
    [Google Scholar]
  6. Green P. N., Bousfield I. J. 1983; Emendation of Methylobacterium (Patt, Cole and Hanson 1976); Methylobacterium rhodinum (Heumann 1962) comb. nov. corrig.; Methylobacterium radiotolerans (Ito and Iizuka, 1971) comb. nov. corrig.; and Methylobacterium mesophilicum (Austin and Goodfellow 1979) comb. nov. International Journal of Systematic Bacteriology 33:875–877
    [Google Scholar]
  7. Haas D., Holloway B. W. 1976; R factor variants with enhanced sex factor activity in Pseudomonas aeruginosa . Molecular and General Genetics 144:243–251
    [Google Scholar]
  8. Holloway B. W. 1981; The application of pseudo-monad-based genetics to methylotrophs. In Micro-bial Growth on C1 Compounds317–324 Dalton H. London: Heyden & Son;
    [Google Scholar]
  9. Kleckner N. 1981; Transposable elements in prokaryotes. Annual Review of Genetics 15:341–404
    [Google Scholar]
  10. MacLennan D. G., Ousby J. G., Vasey R. B., Colton N. T. 1971; The influence of dissolved oxygen on Pseudomonas aeruginosa AM1 grown on methanol in continuous culture. Journal of General Microbiology 69:395–404
    [Google Scholar]
  11. O’Connor M. L., Hanson R. S. 1978; Linkage relationships between mutants of Methylobacterium organophilum impaired in their ability to grow on one-carbon compounds. Journal of General Microbiology 104:105–111
    [Google Scholar]
  12. Peel D., Quayle J. R. 1961; Microbial growth on C1 compounds. I. Isolation and characterization of Pseudomonas AM1. Biochemical Journal 81:465–469
    [Google Scholar]
  13. Sinclair M. I., Holloway B. W. 1982; A chromosomally located transposon in Pseudomonas aeruginosa . Journal of Bacteriology 151:569–579
    [Google Scholar]
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