1887

Abstract

Restriction endonuclease-digested DNAs from several isolates of phase I and phase II were compared using agarose gel electrophoresis and soft-laser scanning densitometry. Our results demonstrate that the two phases are, as previously assumed, alternative phases of the same organism. Although the restriction endonuclease digestion revealed genetic differences between clonal isolates of phase I and phase II Nine Mile strain, these differences do not appear to be related to antigenic phase variation. However, analyses of the fragment patterns generated by restriction enzyme digestion suggest potential grouping of the different isolates.

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1985-06-01
2024-03-28
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References

  1. Baca O. G., Paretsky D. 1983; Q fever and Coxiella burnetii: a model for host-parasite interactions. Microbiological Reviews 47:127–149
    [Google Scholar]
  2. Baca O. G., Martinez I. L., Aragon A. S., Klassen D. 1980; Isolation and partial characterization of a lipopolysaccharide from phase II Coxiella burnetii . Canadian Journal of Microbiology 26:819–826
    [Google Scholar]
  3. Baca O. G., Akporiaye E. T., Aragon A. S., Martinez I. L., Robles M. V., Warner N. L. 1981; Fate of phase I and phase II Coxiella burnetii in several macrophage-like tumor cell lines. Infection and Immunity 33:258–266
    [Google Scholar]
  4. Darai G., Zoller L., Matz B., Delius H., Speck P. T., Flugel R. M. 1982; Analysis of Mycoplasma hyorhinis genome by use of restriction endonucleases and by electron microscopy. Journal of Bacteriology 150:788–794
    [Google Scholar]
  5. Fiset P. 1957; Phase variation of Rickettsia (Coxiella) burnetii. Study of the antibody response in guinea pigs and rabbits. Canadian Journal of Microbiology 3:435–445
    [Google Scholar]
  6. Fiset P. 1964; Serological techniques. In Techniques in Experimental Virology225–255 Harris R. J. C. London: Academic Press;
    [Google Scholar]
  7. Hoyer G. H., Ormsbee R. A., Fiset P., Lackman D. B. 1963; Differentiation of phase I and phase II Coxiella burnetii by equilibrium density gradient sedimentation. Nature, London 197:573–574
    [Google Scholar]
  8. Jerrels T. R., Hinrichs D. J., Mallavia L. P. 1974; Cell envelope analysis of Coxiella burnetii phase I and phase II. Canadian Journal of Microbiology 20:1465–1470
    [Google Scholar]
  9. Kazar J., Brezina R., Schramek S., Urvolgvi J., Pospisil V., Kovacova E. 1974; Virulence, antigenic properties and physicochemical characteristics of Coxiella burnetii strains with different chick embryo yolk sac passage history. Ada virologica 18:434–442
    [Google Scholar]
  10. Kazar J., Skultetyova E., Brezina R. 1975; Phagocytosis of Coxiella burnetii by macrophages. Ada virologica 19:426–431
    [Google Scholar]
  11. Kishimoto R. A., Walker J. S. 1976; Interaction between Coxiella burnetii and guinea pig peritoneal macrophages. Injection and Immunity 14:416–421
    [Google Scholar]
  12. Kraus H., Schiefer H. G., Schmatz H. D. 1977; Ultrastructural investigations on surface structures involved in Coxiella burnetii phase variation. Infection and Immunity 15:890–896
    [Google Scholar]
  13. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning. A Laboratory Manual 161 Cold Spring Harbor, New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  14. McClenaghan M., Herring A. J., Aitken I. D. 1984; Comparison of Chlamydia psittaci isolates by DNA restriction endonuclease analysis. Injection and Immunity 45:384–389
    [Google Scholar]
  15. Myers W. F., Baca O. G., Wisseman C. L. Jr 1980; Genome size of the rickettsia Coxiella burnetii . Journal of Bacteriology 144:460–461
    [Google Scholar]
  16. O’Rourke A. T., Samuel J. E., Natvig D. O., Frazier M. E., Mallavia L. P., Baca O. 1984; Restriction endonuclease analysis of phase I and phase II Coxiella burnetii DNA. Abstracts of the Annual Meeting of the American Society for Microbiology Abstract no. D67 62
    [Google Scholar]
  17. Regnery R. L., Tzianabos T., Esposito J. J., McDade J. E. 1983; Strain differentiation of epidemic typhus rickettsiae (Rickettsia prowazekii) by DNA restriction endonuclease analysis. Current Microbiology 8:355–358
    [Google Scholar]
  18. Schleif R. F., Wensink P. C. 1981 Practical Methods in Molecular Biology New York: Springer-Verlag;
    [Google Scholar]
  19. Schramek S., Mayer H. 1982; Different sugar compositions of lipopolysaccharides isolated from phase I and pure phase II cells of Coxiella burnetii . Injection and Immunity 38:53–57
    [Google Scholar]
  20. Vodkin M., Williams J., Stephenson E. 1985; Deletion of chromosomal DNA fragment between phase I and phase II Coxiella burnetii . Abstracts of the Annual Meeting of the American Society for Microbiology Abstract: no. 128 151
    [Google Scholar]
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