1887

Abstract

IncHl plasmids and the F plasmid of display one-way compatibility. An entering IncHl plasmid is incompatible with a resident F plasmid, but is compatible when it is the resident plasmid. There is little molecular homology between IncH1 plasmids and the F plasmid. A single 5 MDal RI restriction enzyme fragment from digests of several IncHl plasmids hybridizes with probes constructed from the primary replication region of F. Homology can be demonstrated only with the gene for the essential replication protein of F (gene E), but the expression of incompatibility behaviour appears to be associated with the presence of the secondary replicon of the F plasmid. Thus R27 and F are compatible under growth conditions allowing replication and maintenance of F by the secondary replicon. However, a mutant F plasmid which lacks the secondary replicon of F is incompatible with R27 in both directions, irrespective of the growth conditions used.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-131-6-1523
1985-06-01
2022-07-01
Loading full text...

Full text loading...

/deliver/fulltext/micro/131/6/mic-131-6-1523.html?itemId=/content/journal/micro/10.1099/00221287-131-6-1523&mimeType=html&fmt=ahah

References

  1. Anthony W. M., Deonier R. C, Lee H.-J., Hu S., Ohtsubo E., Davidson N. 1974; Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli. IX. Notes on the deletion mutant of F, FΔ(33–43). Journal of Molecular Biology 89:647–650
    [Google Scholar]
  2. Bergquist P. L., Lane H. E. D., Malcolm L., Downard R. A. 1982; Molecular homology and incompatibility in the IncFI plasmid group. Journal of General Microbiology 128:223–238
    [Google Scholar]
  3. Chabbert Y. A., Gerbaud G. R. 1974; Surveillance épidémiologique des plasmides résponsables de la résistance au chloramphénicol de Salmonella typhi . Annales de Microbiologie 125A:153–166
    [Google Scholar]
  4. Coetzee J. N., Datta N., Hedges R. W. 1972; R factors from Proteus rettgeri . Journal of General Microbiology 72:543–552
    [Google Scholar]
  5. Coetzee J. N., Jacob A. E., Hedges R. W. 1975; Susceptibility of a hybrid plasmid to excision of genetic material. Molecular and General Genetics 140:7–14
    [Google Scholar]
  6. Currier T. C., Nester E. W. 1976; Isolation of covalently closed circular DNA of high molecular weight from bacteria. Analytical Biochemistry 76:431–441
    [Google Scholar]
  7. Datta N., Olarte J. 1974; R factors in strains of Salmonella typhi and Shigella dysenteriae I isolated during epidemics in Mexico: classification by incompatibility. Antimicrobial Agents and Chemotherapy 5:310–317
    [Google Scholar]
  8. Falkow S., Guerry P., Hedges R. W., Datta N. 1974; Polynucleotide sequence relationships among plasmids of the I compatibility complex. Journal of General Microbiology 85:65–76
    [Google Scholar]
  9. Gardner R. C, Caughey P. A., Lane D., Bergquist P. L. 1980; Replication mutants of the F-plasmid of Escherichia coli. II. Cloned replication regions of temperature-sensitive mutants. Plasmid 3:179–192
    [Google Scholar]
  10. Gardner R. C, Malcolm L., Bergquist P. L., Lane H. E. D. 1982; IncD, a genetic locus in F responsible for incompatibility with several plasmids of the IncFI group. Molecular and General Genetics 188:345–352
    [Google Scholar]
  11. Grindley N. D. F., Grindley J. N., Anderson E. S. 1972; R factor incompatibility groups. Molecular and General Genetics 119:287–297
    [Google Scholar]
  12. Hyman R. W., Brunovskis I., Summers W. C. 1973; DNA base sequence homology between coliphages T7 and ϕII and between T3 and ϕII as determined by heteroduplex mapping in the electron microscope. Journal of Molecular Biology 77:189–196
    [Google Scholar]
  13. Kline B. C., Trawick J. 1983; Identification and characterization of a second copy number control gene in mini-F plasmids. Molecular and General Genetics 192:408–415
    [Google Scholar]
  14. Lane D., Gardner R. C. 1979; Second EcoRI fragment of F capable of self-replication. Journal of Bacteriology 139:141–151
    [Google Scholar]
  15. Lane H. E. D. 1981; Replication and incompatibility of F and plasmids in the Inc-FI group. Plasmid 5:100–126
    [Google Scholar]
  16. Lovett M. A., Helinski D. 1976; Method for the isolation of the replication region of a bacterial replicon: construction of a mini-F'km plasmid. Journal of Bacteriology 127:982–989
    [Google Scholar]
  17. Murotsu T., Matsubara K., Sugisahi H., Takanami M. 1981; Nine unique repeating sequences in a region essential for replication and incompatibility of the mini-F plasmid. Gene 15:257–271
    [Google Scholar]
  18. Rigby P. W. J., Dieckmann M., Rhodes C., Berg P. 1977; Labelling deoxyribonucleic acid to high specific activity in vitro by nick-translation with DNA polymerase I. Journal of Molecular Biology 113:237–251
    [Google Scholar]
  19. Roussel A. F., Chabbert Y. A. 1978; Taxonomy and epidemiology of Gram-negative bacterial plasmids studied by DNA-DNA filter hybridization in formamide. Journal of General Microbiology 104:269–276
    [Google Scholar]
  20. Smith H. R., Grindley N. D. F., Humphreys G. O., Anderson E. S. 1973; Interactions of group H resistance factors with the F factor. Journal of Bacteriology 115:623–628
    [Google Scholar]
  21. SØgaard-Andersen L., Rokeach L. A., Molin S. 1984; Regulated expression of a gene important for replication of plasmid F in E. coli . EM BO Journal 3:257–262
    [Google Scholar]
  22. Taylor D. E. 1983; Transfer-defective and tetra-cycline sensitive mutants of the incompatibility group HI plasmid R27 generated by insertion of transposon 7. Plasmid 9:227–239
    [Google Scholar]
  23. Watson L. A., Phua S.-H., Bergquist P. L., Lane H. E. D. 1982; An Mr 29,000 protein is essential for mini-F maintenance in E. coli . Gene 19:173–178
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-131-6-1523
Loading
/content/journal/micro/10.1099/00221287-131-6-1523
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error