1887

Abstract

strain FR-19 lysed spontaneously in substrate-depleted cultures. The addition of 0.3 -sucrose prevented complete lysis and resulted in the formation of osmotically sensitive UV-fluorescent spheres. Electron microscopic examination showed that complete degradation of the cell wall occurred before the release of true protoplasts, which were stabilized by sucrose and glucose (0·2–0·3 ) but not by glycerol. Exponentially increasing methane production and regeneration of protoplasts could not be demonstrated.

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/content/journal/micro/10.1099/00221287-131-6-1481
1985-06-01
2021-08-02
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