RT Journal Article SR Electronic(1) A1 STAEBELL, MARGARET A1 SOLL*, DAVID R.YR 1985 T1 Temporal and Spatial Differences in Cell Wall Expansion during Bud and Mycelium Formation in Candida albicans JF Microbiology, VO 131 IS 6 SP 1467 OP 1480 DO https://doi.org/10.1099/00221287-131-6-1467 PB Microbiology Society, SN 1465-2080, AB The infectious yeast Candida albicans is capable of growing in either a budding or mycelium form, depending upon the pH of the supporting medium. By monitoring the position of polylysine-coated beads firmly attached to the wall of growing cells, the zones of expansion for the surface of the cell wall have been mapped for the alternative growth forms. Both spatial and temporal differences are demonstrated to exist. During roughly the first two-thirds of bud growth, a very small, highly active apical zone accounts for roughly 70% of surface expansion. The remaining 30% is due to general expansion. When a bud reaches approximately two-thirds of its final surface area, the apical zone shuts down, and subsequent expansion is completed by the general mechanism. During mycelial growth, at least 90% of expansion is due to a small, highly active apical growth zone, and less than 10% is due to the general mechanism. In contrast to budding cells, the apical zone of the growing mycelium never shuts down as long as growth continues in the mycelial form. These distinct temporal and spatial differences in expansion are considered in terms of the regulation of alternative phenotypes in Candida., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-131-6-1467