Vegetative cells of were induced to encyst in the presence of β-hydroxybutyrate. Although modal cell volume fluctuated (2.7 to 4 μ;m) markedly during exponential growth, the final modal volume attained by mature cysts (1.13 to 1.3 μ;m) was relatively constant. Respiratory activity of encysting cultures fell rapidly 3 h after initiation of encystment. This was accompanied by decreased inhibition of respiration by KCN. NADH oxidase activity of membrane preparations fell from approximately 450 to 50 nmol O consumed min (mg protein) during differentiation and was also less sensitive to KCN. Difference spectra of cytochromes in membrane preparations revealed a number of changes. Amounts of cytochromes and all fell during encystment; cytochrome levels increased whilst cytochrome oxidase was gradually lost from the membrane during the first 22 h of morphogenesis.


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