1887

Abstract

Vegetative cells of were induced to encyst in the presence of -hydroxybutyrate. Although modal cell volume fluctuated (2·7 to 4 μm) markedly during exponential growth, the final modal volume attained by mature cysts (1·13 to 1·3 μm) was relatively constant. Respiratory activity of encysting cultures fell rapidly 3 h after initiation of encystment. This was accompanied by decreased inhibition of respiration by KCN. NADH oxidase activity of membrane preparations fell from approximately 450 to 50 nmol O consumed min (mg protein) during differentiation and was also less sensitive to KCN. Difference spectra of cytochromes in membrane preparations revealed a number of changes. Amounts of cytochromes and all fell during encystment; cytochrome levels increased whilst cytochrome oxidase was gradually lost from the membrane during the first 22 h of morphogenesis.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-131-6-1403
1985-06-01
2024-04-24
Loading full text...

Full text loading...

/deliver/fulltext/micro/131/6/mic-131-6-1403.html?itemId=/content/journal/micro/10.1099/00221287-131-6-1403&mimeType=html&fmt=ahah

References

  1. Campbell A. C. 1957; Synchronization of cell division. Bacteriological Reviews 21:263–272
    [Google Scholar]
  2. Erickson S. R., Diehl H. 1973; The terminal oxidases of Azotobacter vinelandii. Biochemical and Biophysical Research Communications 50:321–327
    [Google Scholar]
  3. Field A. M., Edwards C. 1983; Respiratory properties of cysts and vegetative cells of Azotobacter vinelandii. FEMS Microbiology Letters 20:113–117
    [Google Scholar]
  4. Gorin P. A. J., Spencer J. F. T. 1966; Exocellular alginic acid from Azotobacter vinelandii. Canadian Journal of Biochemistry 44:993–999
    [Google Scholar]
  5. Haddock B. A., Jones C. W. 1977; Bacterial respiration. Bacteriological Reviews 41:47–99
    [Google Scholar]
  6. Hitchins V. M., Sadoff H. L. 1970; Morphogenesis of cysts in Azotobacter vinelandii. Journal of Bacteriology 104:492–498
    [Google Scholar]
  7. Hitchins V. M., Sadoff H. L. 1973; Sequential metabolic events during encystment of Azotobacter vinelandii. Journal of Bacteriology 113:1273–1279
    [Google Scholar]
  8. Hoffman P. S., Morgan T. V., Dervartanian D. V. 1979; Respiratory-chain characteristics of mutants of Azotobacter vinelandii negative to tetra-methyl-p-phenylenediamine oxidase. European Journal of Biochemistry 100:19–27
    [Google Scholar]
  9. Hoffman P. S., Irwin R. M., Carreira L. A., Morgan T. V., Ensley B. D., Dervartanian D. V. 1980a; Studies of photochemical action spectra on N, N, N′, N′-tetramethyl-p-phenylenedi-amine oxidase-negative mutants of Azotobacter vinelandii. European Journal of Biochemistry 105:177–185
    [Google Scholar]
  10. Hoffman P. S., Morgan T. V., Dervartanian D. V. 1980b; Respiratory properties of cytochrome c-deficient mutants of Azotobacter vinelandii. European Journal of Biochemistry 110:349–354
    [Google Scholar]
  11. Jarman T. R., Deavin L., Slocombe S., Righe-lato R. C. 1978; Investigation of the effect of environmental conditions on the rate of exopolysac-charide synthesis in Azotobacter vinelandii. Journal of General Microbiology 107:59–64
    [Google Scholar]
  12. Jones C. W., Brice J. M., Wright V., Ackrell B. A. C. 1973; Respiratory protection of nitrogenase in Azotobacter vinelandii. FEBS Letters 29:77–80
    [Google Scholar]
  13. Jurtshuk P., Yang T. 1980; Oxygen reactive haemoprotein components in bacterial respiratory systems. In Diversity of Bacterial Respiratory Systems 1137–159 Knowles C. J. Boca Raton: CRC Press;
    [Google Scholar]
  14. Kauffman H. F., Van Gelder B. F. 1974; The respiratory chain of Azotobacter vinelandii. III. The effect of cyanide in the presence of substrates. Biochimica et biophysica acta 333:218–227
    [Google Scholar]
  15. Lin L. P., Sadoff H. L. 1968; Encystment and polymer production by Azotobacter vinelandii in the presence of β-hydroxybutyrate. Journal of Bacteriology 95:2336–2343
    [Google Scholar]
  16. Post E., Golecki J. R., Oelze J. 1982; Morphological and ultrastructural variations in Azotobacter vinelandii growing in oxygen-controlled continuous culture. Archives of Microbiology 133:75–82
    [Google Scholar]
  17. Repaske R. 1958; Lysis of Gram-negative organisms and the role of versene. Biochimica et biophysica acta 30:225–232
    [Google Scholar]
  18. Reusch R. N., Sadoff H. L. 1979; 5-n-alkyl resorcinols from encysting Azotobacter vinelandii: isolation and characterization. Journal of Bacteriology 139:448–453
    [Google Scholar]
  19. Reusch R. N., Sadoff H. L. 1981; Lipid metabolism during encystment of Azotobacter vinelandii. Journal of Bacteriology 145:889–895
    [Google Scholar]
  20. Reusch R. N., Sadoff H. L. 1983; Novel lipid components of the Azotobacter vinelandii cyst membrane. Nature, London 302:268–270
    [Google Scholar]
  21. Sadoff H. L. 1975; Encystment and germination in Azotobacter vinelandii. Bacteriological Reviews 39:516–539
    [Google Scholar]
  22. Socolofsky M. D., Wyss O. 1961; Cysts of Azotobacter. Journal of Bacteriology 81:946–954
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-131-6-1403
Loading
/content/journal/micro/10.1099/00221287-131-6-1403
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error