1887

Abstract

Vegetative cells of were induced to encyst in the presence of -hydroxybutyrate. Although modal cell volume fluctuated (2·7 to 4 μm) markedly during exponential growth, the final modal volume attained by mature cysts (1·13 to 1·3 μm) was relatively constant. Respiratory activity of encysting cultures fell rapidly 3 h after initiation of encystment. This was accompanied by decreased inhibition of respiration by KCN. NADH oxidase activity of membrane preparations fell from approximately 450 to 50 nmol O consumed min (mg protein) during differentiation and was also less sensitive to KCN. Difference spectra of cytochromes in membrane preparations revealed a number of changes. Amounts of cytochromes and all fell during encystment; cytochrome levels increased whilst cytochrome oxidase was gradually lost from the membrane during the first 22 h of morphogenesis.

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1985-06-01
2021-10-27
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