1887

Abstract

A glycosidic linkage between the arabinogalactan and peptidoglycan has been demonstrated for the first time in cell walls from AN-115. Two oligosaccharides, Rha-(1→3)-GlcNAc and Gal-Rha-(1→3)-GlcNAc, were identified by gas chromatography-mass spectrometry in acid hydrolysates of arabinogalactan containing defective peptidoglycan polymer obtained from stepwise alkaline degradations of AN-115 walls. The arabinogalactan of AN-115 walls contained not only Ara and Gal but also Rha, while peptidoglycan contained glycan polymers composed of GlcNAc-MurNG1 units. These findings strongly suggest that arabinogalactan was linked glycosidically to peptidoglycan through a Rha-GlcN Ac linkage in AN-115 walls.

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/content/journal/micro/10.1099/00221287-131-6-1323
1985-06-01
2021-10-27
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References

  1. Abou-Zeid C., Voiland A., Michel G., Cocito C. 1982; Structure of the wall polysaccharide isolated from a group of Corynebacteria . European Journal of Biochemistry 128:363–370
    [Google Scholar]
  2. Azuma I., Kanetsuna F., Taniyama T., Yamamura Y., Hori M., Tanaka Y. 1975; Adjuvant activity of mycobacterial fractions. I. Purification and in vivo adjuvant activity of cell wall skeletons of Myocobacterium bovis BCG, Nocardia asteroides 131 and Corvnebacterium diphtheriae PW8. Biken Journal 18:1–13
    [Google Scholar]
  3. Azuma I., Taniyama T., Sugimura K., Aladin A. A., Yamamura Y. 1976; Mitogenic activity of the cell walls of Mycobacteria Nocardia Corynebacteria and anaerobic coryneforms. Japanese Journal of Microbiology 20:263–271
    [Google Scholar]
  4. Barksdale L., Kim K.-S. 1977; Mycobacterium . Bacteriological Reviews 41:217–372
    [Google Scholar]
  5. Boltz D. F., Lueck C. H. 1958; Phosphorus. In Colorimetric Determination of Nonmetals29 Boltz D. F. New York: Interscience;
    [Google Scholar]
  6. Cummins C. S. 1962; Chemical composition and antigenic structure of cell walls of Corynebacterium Mycobacterium, Nocardia, Actinomyces and Arthrobacter. Journal of General Microbiology 28:35–50
    [Google Scholar]
  7. Cunto G., Kanetsuna F., Imaeda T. 1969; Chemical analysis of the mucopeptide of Mycobacterium smegmatis . Biochimica et biophysica acta 192:358–360
    [Google Scholar]
  8. Hakomori S. 1964; A rapid permethylation of glycolipid and polysaccharide catalyzed by methyl sulfinyl carbanion in dimethyl sulfoxide. Journal of Biochemistry 55:205–208
    [Google Scholar]
  9. Kanetsuna F. 1968; Chemical analyses of mycobacterial cell walls. Biochimica et biophysica acta 158:130–143
    [Google Scholar]
  10. Kanetsuna F., San Blas G. 1970; Chemical analysis of a mycolic acid-arabinogalactan-muco-peptide complex of mycobacterial cell wall. Biochimica et biophysica acta 208:434–443
    [Google Scholar]
  11. Kärkkäinen J. 1970; Analysis of disaccharides as permethylated disaccharide alditols by gas-liquid chromatography-mass spectrometry. Carbohydrate Research 14:27–33
    [Google Scholar]
  12. Kärkkäinen J. 1971; Structural analysis of trisaccharides as permethylated trisaccharide alditols by gas-liquid chromatography-mass spectrometry. Carbohydrate Research 17:11–18
    [Google Scholar]
  13. Kotani S., Yanagida I., Kato K., Matsuda T. 1970; Studies on peptides, glycopeptides and antigenic polysaccharide-glycopeptide complexes isolated from an L-11 enzyme lysate of the cell walls of Mycobacterium tuberculosis strain H37Rv. Biken Journal 13:249–275
    [Google Scholar]
  14. Lederer E. 1971; The mycobacterial cell wall. Pure and Applied Chemistry 25:135–165
    [Google Scholar]
  15. Liu T.-Y., Gotschlich E. 1967; Muramic acid phosphate as a component of the mucopeptide of gram-positive bacteria. Journal of Biological Chemistry 242:471–476
    [Google Scholar]
  16. Lönngren J., Svensson S. 1974; Mass spectrometry in structural analysis of natural carbohydrates. Advances in Carbohydrate Chemistry and Biochemistry 29:41–106
    [Google Scholar]
  17. Lundblad A., Masson P. K., Norden N. E., Svenssen S., Ockerman P. A. 1975; Determination of mannose-containing trisaccharide in the urine of patients and heterozygotes of mannosidosis. Biomedicai Mass Spectrometry 2:285–287
    [Google Scholar]
  18. Michel G., Bordet C. 1976; Cell wall of Nocardiae . In The Biology of the Nocardiae141–159 Goodfellow M., Brownell G. M., Serrano J. A. London: Academic Press;
    [Google Scholar]
  19. Mononen L., Finne J., Kärkkäinen J. 1978; Analysis of permethylated hexopyranosyl-2-aceta-mide-2-deoxyhexitols by g.1.c.–m.s. Carbohydrate Research 60:371–375
    [Google Scholar]
  20. Yamaguchi T. 1965; Comparison of the cell-wall composition of morphologically distinct actinomycetes. Journal of Bacteriology 89:444–453
    [Google Scholar]
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