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A glycosidic linkage between the arabinogalactan and peptidoglycan has been demonstrated for the first time in cell walls from Nocardia rubra AN-115. Two oligosaccharides, Rha-(1→3)-GlcNAc and Gal-Rha-(1→3)-GlcNAc, were identified by gas chromatography-mass spectrometry in acid hydrolysates of arabinogalactan containing defective peptidoglycan polymer obtained from stepwise alkaline degradations of N. rubra AN-115 walls. The arabinogalactan of N. rubra AN-115 walls contained not only Ara and Gal but also Rha, while peptidoglycan contained glycan polymers composed of GlcNAc-MurNG1 units. These findings strongly suggest that arabinogalactan was linked glycosidically to peptidoglycan through a Rha-GlcN Ac linkage in N. rubra AN-115 walls.
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