The exclusion relationship between the IncI plasmids R144, R64 and ColIb was studied in such a way that incompatibility interference was avoided. Genetic crosses with an R144-derived Hfr donor, crosses with recipient strains carrying R144-derived exclusion genes on a recombinant plasmid compatible with R144, and measurement of transmission frequencies of a recombinant plasmid compatible with IncI plasmids after mobilization by R144 revealed that R144, R64 and ColIb belong to one exclusion group.
AchtmanM.,
SkurrayR.1977; A redefinition of the mating phenomenon in bacteria. In Microbial Interactions : Receptors and Recognition, Series B3233–279ReissigJ. L.
London: Chapman & Hall;
BolivarF.,
RodriguezR. L.,
BetlachM. C,
HeynekerH. L.,
BoyerH. W.,
CrosaJ. H.,
FalkowS.1977; Construction and characterization of new cloning vehicles II. A multipurpose cloning system. Gene 2:95–113
ChangA. C. Y.,
CohenS. N.1978; Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid. Journal of Bacteriology 134:1141–1156
ClarkeL.,
CarbonJ.1978; Functional expression of cloned yeast DNA in Escherichia coli: specific complementation of argininosuccinate lyase (argH) mutations. Journal of Molecular Biology 120:517–532
HartskeerlR. A.,
HoekstraW. P. M.1984; Exclusion in Incl-type Escherichia coli conjugations: the stage of conjugation at which exclusion operates. Antonie ran Leeuwenhoek 50:113–124
HartskeerlR. A.,
BergmansJ. E. N.,
KampM. C.,
HoekstraW. P. M.1983; Cloning of an exclusion-determining fragment of the IncI plasmid R144. Plasmid 10:11–20
HartskeerlR. A., van der GuchteM.,
ZuidwegE. M.,
HoekstraW. P. M.1984; Physical and genetic characterization of the IncI plasmid R144. drd3. Plasmid 12:215–217
HavekesL.,
TommassenJ.,
HoekstraW.,
LugtenbergB.1977; Isolation and characterization of Escherichia coli K12 F‒ mutants defective in conjugation with an I-type donor. Journal of Bacteriology 129:1–8
MeynellE.,
CookeM.1969; Repressor-minus and operator constitutive de-repressed mutants of F-like R factors. Their effect on chromosomal transfer by HfrC. Genetical Research 14:309–313
WilkinsB. M.,
HollomS. E.,
RuppW. D.1971; Deoxyribonucleic acid transferred from ultraviolet-irradiated excision-defective Hfr cells of Escherichia coli K-12. Journal of Bacteriology 107:505–512
WinklerK. C.,
de HaanP. G.1948; On the action of sulfanilamide. XII. A set of non-competitive sulfanilamide antagonists for Escherichia coli. Archives of Biochemistry 18:97–107