RT Journal Article SR Electronic(1) A1 ODGERS, J. A1 DYER, J. K. A1 MARTIN, E. L. A1 BOLTON, R. W. A1 ANG, B. J.YR 1985 T1 Induction of Spheroplasts in Capnocytophaga ochracea JF Microbiology, VO 131 IS 5 SP 993 OP 999 DO https://doi.org/10.1099/00221287-131-5-993 PB Microbiology Society, SN 1465-2080, AB A gentle technique for preparing spheroplasts of Capnocytophaga ochracea strain 25 is described. Cells in the exponential phase were washed with 1·0 m-NaCl, agitated in 1·0 m-NaCl for 2 h at 30°C and exposed to lysozyme in a Tris/salts buffer, pH 7·0. This procedure resulted in 98% spheroplast formation with complete removal of the peptidoglycan layer as detected by both phase-contrast and electron microscopy in combination with chemical analysis., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-131-5-993