1887

Abstract

A gentle technique for preparing spheroplasts of strain 25 is described. Cells in the exponential phase were washed with 1·0 -NaCl, agitated in 1·0 -NaCl for 2 h at 30°C and exposed to lysozyme in a Tris/salts buffer, pH 7·0. This procedure resulted in 98% spheroplast formation with complete removal of the peptidoglycan layer as detected by both phase-contrast and electron microscopy in combination with chemical analysis.

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1985-05-01
2021-07-26
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References

  1. Bick P. H., Carpenter A. B., Holdeman L. V., Miller G. A., Ranney R. R., Polcanis K. G., Tew J. G. 1981; Polyclonal B-cell activation induced by extracts of Gram-negative bacteria isolated from periodontally diseased sites. Infection and Immunity 34:43–49
    [Google Scholar]
  2. Celesk R. A., McCabe R. M., London J. 1979; Colonization of the cementum surface of teeth by oral Gram-negative bacteria. Infection and Immunity 26:15–18
    [Google Scholar]
  3. Costerton J. W., Forsberg C, Mitula T. I., Buckmire F. L. A., MacLeod R. A. 1967; Nutrition and metabolism of marine bacteria. XVI.Formation of protoplasts, spheroplasts and related forms from a Gram-negative marine bacterium. Journal of Bacteriology 94:1764–1777
    [Google Scholar]
  4. Forsberg C. W., Costerton J. W., MacLeod R. A. 1970a; Separation and localization of cell wall layers of a Gram-negative bacterium. Journal of Bacteriology 104:1338–1353
    [Google Scholar]
  5. Forsberg C. W., Costerton J. W., MacLeod R. A. 1970a; Quantitation, chemical characteristics, and ultrastructure of the three outer cell wall layers of a Gram-negative bacterium. Journal of Bacteriology 104:1354–1368
    [Google Scholar]
  6. Hadzija O. 1974; A simple method for the quantitative determination of muramic acid. Analytical Biochemistry 60:512–517
    [Google Scholar]
  7. Hammond B. F., tevens R. H. 1982; Capnocytophaga and Actinobacillus actinomycetemcomitans : occurrence and pathogenic potential in juvenile periodontitis. In Host Parasite Interactions in Periodontal Diseases46–61 Genco R. J., Mergenhagen S. E. Washington DC:: American Society for Microbiology;
    [Google Scholar]
  8. Holt S. C. 1982; Bacterial surface structures and their role in periodontal disease. In Host-Parasite Interactions in Periodontal Diseases139–150 Genco R. J., Mergenhagen S. E. Washington: DC:American Society for Microbiology;
    [Google Scholar]
  9. Holt S. C, Leadbetter E. R., Socransky S. S. 1979; Capnocytophaga: new genus of Gram-negative gliding bacteria. II. Morphology and ultrastructure. Archives of Microbiology 122:17–27
    [Google Scholar]
  10. Karkhanis Y. D, Zeltner J. Y. 1978; A new and improved microassay to determine KDO in LPS of Gram-negative bacteria. Analytical Biochemistry 85:595–601
    [Google Scholar]
  11. Laddaga R. A., MacLeod R. A. 1983; Factors affecting the release of outer membrane from a Gram-negative marine bacterium. Canadian Journal of Microbiology 29:659–663
    [Google Scholar]
  12. Leadbetter E. R., Holt S. C., Socransky S. S. 1979; Capnocytophaga: a new genus of Gram-negative gliding bacteria. I. General characteristics, taxonomic considerations and significance. Archives of Microbiology 122:9–16
    [Google Scholar]
  13. Lindhe J., Socransky S. S. 1979; Chemotaxis and vascular permeability produced by human periodon-topathic bacteria. Journal of Periodontal Research 14:138–146
    [Google Scholar]
  14. Markwell M. A., Haas S. M., Bieber L. L., Tobert N. E. 1978; A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples. Analytical Biochemistry 87:207–210
    [Google Scholar]
  15. Newman M. G. 1979 The role of microorganisms in the etiology of gingival and periodontal disease. In Glickman’s Clinical Periodontology, 5.74–405 Carranza F. A. Philadelphia: W. B. Saunders Co;
    [Google Scholar]
  16. Newman M. G., Sims T. N. 1979; The predominant cultivable microbiota of the periodontal abscess. Journal of Periodontology 50:350–354
    [Google Scholar]
  17. Newman M. G., Socransky S. S. 1977; Predominant cultivable microbiota in periodontosis. Journal of Periodontal Research 12:120–128
    [Google Scholar]
  18. Newman M. G., Socransky S. S., Savitt E. D., Propos D., Crawford A. 1976; Studies on the microbiology of periodontosis. Journal of Periodontal Research 12:120–128
    [Google Scholar]
  19. Poirier T. P., Holt S. C. 1983; Acid and alkaline phosphatases of Capnocytophaga species. III. The relationship of the enzymes to the cell wall. Canadian Journal of Microbiology 29:1369–1381
    [Google Scholar]
  20. Poirier T. P., Tonelli S. J., Holt S. C. 1979; Ultrastructure of gliding bacteria : scanning electron microscopy of Capnocytophaga sputigena, Capnocytophaga gingivalis and Capnocytophaga ochracea. Infection and Immunity 26:1146–1158
    [Google Scholar]
  21. Sato T. 1968; A modified method for lead staining of thin sections. Journal of Electron Microscopy 17:158.
    [Google Scholar]
  22. Shurin S. B., Socransky S. S., Sweeney E., Stossel T. P. 1979; A neutrophil disorder induced by Capnocytophaga, a dental microorganism. New England Journal of Medicine 301:849–854
    [Google Scholar]
  23. Slots J. 1976; The predominant cultivable organisms in juvenile periodontitis. Scandinavian Journal of Dental Research 84:1–10
    [Google Scholar]
  24. Slots J. 1977; The predominant cultivable microflora of advanced periodontitis. Scandinavian Journal of Dental Research 85:14–121
    [Google Scholar]
  25. Socransky S. S., Holt S. C, Leadbetter E. R., Tanner A. C. R., Hammond B. F. 1979; Capnocytophaga: new genus of Gram-negative gliding bacteria. III. Physiological characterization. Archives of Microbiology 122:29–33
    [Google Scholar]
  26. Stevens R. H., Sela M. N., Shapira J., Hammond B. F. 1980; Detection of fibroblast proliferation inhibitory factor from Capnocytophaga sputigena. Infection and Immunity 27:271–275
    [Google Scholar]
  27. Van Palenstein Helderman W. H. 1981; Microbial etiology of periodontal disease. Journal of Clinical Periodontology 8:261–280
    [Google Scholar]
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