1887

Abstract

A gentle technique for preparing spheroplasts of strain 25 is described. Cells in the exponential phase were washed with 1·0 -NaCl, agitated in 1·0 -NaCl for 2 h at 30°C and exposed to lysozyme in a Tris/salts buffer, pH 7·0. This procedure resulted in 98% spheroplast formation with complete removal of the peptidoglycan layer as detected by both phase-contrast and electron microscopy in combination with chemical analysis.

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1985-05-01
2024-04-23
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