RT Journal Article SR Electronic(1) A1 HUGOUVIEUX-COTTE-PATTAT*, N. A1 ROBERT-BAUDOUY, J.YR 1985 T1 Isolation of kdgK-lac and kdgA-lac Gene Fusions in the Phytopathogenic Bacterium Erwinia chrysanthemi JF Microbiology, VO 131 IS 5 SP 1205 OP 1211 DO https://doi.org/10.1099/00221287-131-5-1205 PB Microbiology Society, SN 1465-2080, AB Erwinia chrysanthemi is a species of enterobacteria whose phytopathogenicity is mainly due to its pectinolytic activity. Pectin degradation leads to the formation of 2-keto-3-deoxygluconate which is catabolized via the kdgA and kdgK gene products. Mutants of Er. chrysanthemi containing genetic fusions of the kdgK or kdgA genes to the lacZ gene of Escherichia coli were isolated by infection of a lacZ mutant of Er. chrysanthemi with the phage Mu d(Ap lac). In these fusion strains, the absence of growth on galacturonate, glucuronate and polygalacturonate, and also β-galactosidase expression, are caused by a single Mu d(Ap lac) insertion. Synthesis of β-galactosidase in these strains was induced in the presence of galacturonate, glucuronate, polygalacturonate or some intermediates of the catabolism of these sugars in the culture medium; synthesis of β-galactosidase was not sensitive to glucose repression., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-131-5-1205