is a species of enterobacteria whose phytopathogenicity is mainly due to its pectinolytic activity. Pectin degradation leads to the formation of 2-keto-3-deoxygluconate which is catabolized via the and gene products. Mutants of containing genetic fusions of the or genes to the gene of were isolated by infection of a mutant of with the phage Mu d(Ap ). In these fusion strains, the absence of growth on galacturonate, glucuronate and polygalacturonate, and also β-galactosidase expression, are caused by a single Mu d(Ap ) insertion. Synthesis of β-galactosidase in these strains was induced in the presence of galacturonate, glucuronato, polygalacturonate or some intermediates of the catabolism of these sugars in the culture medium; synthesis of β-galactosidase was not sensitive to glucose repression.


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