RT Journal Article SR Electronic(1) A1 KAGAN, S. A. A1 BREWIN*, N. J.YR 1985 T1 Mutagenesis of a Rhizobium Plasmid Carrying Hydrogenase Determinants JF Microbiology, VO 131 IS 5 SP 1141 OP 1147 DO https://doi.org/10.1099/00221287-131-5-1141 PB Microbiology Society, SN 1465-2080, AB Transposon Tn5-mob, a Tn5 derivative containing the mobilization site of plasmid RP4, was introduced into Rhizobium leguminosarum strain 128C53 on the suicide vector pSup5011. Transposon insertions into the plasmids of strain 128C53 were selected after mating the mutagenized population with a non-nodulating R. leguminosarum recipient. In this way, a tenfold enrichment was effected for plasmid-linked mutations. Transconjugants that had received mutagenized symbiotic plasmids were applied individually to the roots of pea seedlings and, after three weeks, the resulting nodules were screened for the absence of hydrogenase activity. Eight hydrogenase-deficient (Hup–) mutants of R. leguminosarum strain 128C53 were isolated. Physical and genetic analyses suggest that each Hup– mutant was due to a single insertion of Tn5-mob into the symbiotic plasmid, pRL6JI. All mutations were suppressible by pHU1, a cosmid clone carrying some of the hup genes of Rhizobium japonicum., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-131-5-1141