1887

Abstract

Transposon Tn-mob, a Tn derivative containing the mobilization site of plasmid RP4, was introduced into strain 128C53 on the suicide vector pSup5011. Transposon insertions into the plasmids of strain 128C53 were selected after mating the mutagenized population with a non-nodulating . recipient. In this way, a tenfold enrichment was effected for plasmid-linked mutations. Transconjugants that had received mutagenized symbiotic plasmids were applied individually to the roots of pea seedlings and, after three weeks, the resulting nodules were screened for the absence of hydrogenase activity. Eight hydrogenase-deficient (Hup) mutants of . strain 128C53 were isolated. Physical and genetic analyses suggest that each Hup mutant was due to a single insertion of Tn-mob into the symbiotic plasmid, pRL6JI. All mutations were suppressible by pHU1, a cosmid clone carrying some of the genes of .

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-131-5-1141
1985-05-01
2022-08-18
Loading full text...

Full text loading...

/deliver/fulltext/micro/131/5/mic-131-5-1141.html?itemId=/content/journal/micro/10.1099/00221287-131-5-1141&mimeType=html&fmt=ahah

References

  1. Beringer J. E. 1974; R factor transfer in Rhizohium leguminosarum. Journal of General Microbiology 84:188–198
    [Google Scholar]
  2. Beringer J. E., Hoggan S. A., Johnston A. W. B. 1978; Linkage mapping in Rhizohium leguminosarum by means of R plasmid-mediated recombination. Journal of General Microbiology 104:201–207
    [Google Scholar]
  3. Beynon J. L., Beringer J. E., Johnston A. W. B. 1980; Plasmids and host range in Rhizobium leguminosarum and Rhizohium phaseoli. Journal of General Microbiology 120:421–429
    [Google Scholar]
  4. Brewin N. J., DeJong T. M., Phillip D. A., Johnston A. W. B. 1980; Co-transfer of determinants of hydrogenase activity and nodulation ability in Rhizohium leguminosarum. Nature, London 288:77–79
    [Google Scholar]
  5. Brewin N. J., Wood E. A., Johnston A. W. B., Dibb N., Hombrecher G. 1982; Recombinant nodulation plasmids in Rhizobium leguminosarum. Journal of General Microbiology 128:1817–1827
    [Google Scholar]
  6. Brewin N. J., Dibb N. J., Hombrecher G. 1983; Natural variation in Rhizohium plasmids. Molecular Genetics of the Bacteria Plant Interaction113–121 Puhler A. Berlin & Heidelberg: Springer-Verlag;
    [Google Scholar]
  7. Buchanan-Wollaston V. 1979; Generalized transduction in Rhizohium leguminosarum. Journal of General Microbiology 112:135–142
    [Google Scholar]
  8. Buchanan-Wollaston A. V., Beringer J. E., Brewin N. J., Hirsch P. R., Johnston A. W. B. 1980; Isolation of symbiotically defective mutants of Rhizobium leguminosarum by insertion of the transposon Tn5 into a transmissible plasmid. Molecular and General Genetics 178:185–190
    [Google Scholar]
  9. Cantrell M. A., Haugland R. A., Evans H. J. 1983; Construction of a Rhizobium japonicum gene bank and use in the isolation of a hydrogen uptake gene. Proceedings of the National Academy of Sciences of the United States of America 80181–185
    [Google Scholar]
  10. DeJong T. M., Brewin N. J., Johnston A. W. B., Phillips D. A. 1982; Improvement of symbiotic properties in Rhizobium leguminosarum by plasmid transfer. Journal of General Microbiology 128:1829–1838
    [Google Scholar]
  11. Dibb N. J., Downie J. A., Brewin N. J. 1984; The identification of a rhizosphere protein encoded by the symbiotic plasmid of Rhizohium leguminosarum. Journal of Bacteriology 158:621–627
    [Google Scholar]
  12. Ditta G., Stanfield S., Corbin D., Helinski D. R. 1980; Broad host range DNA cloning system for Gram-negative bacteria; construction of a gene bank of Rhizohium meliloti. Proceedings of the National Academy of Sciences of the United States of America 777347–7391
    [Google Scholar]
  13. Downie J. A., Ma Q.-S., Knight C. D., Hombrecher G., Johnston A. W. B. 1983; Cloning of the symbiotic region of Rhizobium leguminosarum: the nodulation genes are between the nitrogenase genes and a nifA-like gene. EMBO Journal 2:947–952
    [Google Scholar]
  14. Eisbrenner G., Evans H. J. 1983; Aspects of hydrogenase metabolism in nitrogen fixing legumes and other plant microbe interactions. Annual Review of Plant Physiology 34:105–136
    [Google Scholar]
  15. Hirsch P. R., van Montagu M., Johnston A. W. B., Brewin N. J., Schell J. 1980; Physical identification of bacteriocinogenic nodulation and other plasmids in strains of Rhizobium leguminosarum. Journal of General Microbiology 120:403–412
    [Google Scholar]
  16. Johnston A. W. B., Beynon J., Buchanan-Wollaston A. V., Setchell S. M., Hirsch P. R., Beringer J. E. 1978; High frequency transfer of nodulating ability between strains and species of Rhizobium. Nature, London 276:635–636
    [Google Scholar]
  17. Lepo J. E., Hickok R. E., Cantrell M. A., Russell S. A., Evans H. J. 1981; Revertible hydrogen uptake-deficient mutants of Rhizobium japonkum. Journal of Bacteriology 146:614–620
    [Google Scholar]
  18. Maier R. J. 1981; Rhizobium japonkum: mutant strains unable to grow chemoautotrophically with H2 . Journal of Bacteriology 145:533–540
    [Google Scholar]
  19. Maier R. J., Campbell N. E. R., Hanus F. J., Simpson F. B., Russell S. A., Evans H. J. 1978; Expression of hydrogenase activity in free-living Rhizobium japonkum. Proceedings of the National Academy of Sciences of the United States of America 75:3258–3262
    [Google Scholar]
  20. Ruiz-Argüeso T., Maier R. J., Evans H. J. 1979; Hydrogen evolution from alfalfa and clover nodules and hydrogen uptake by free-living Rhizobium meliloti. Applied and Environmental Microbiology 37:582–587
    [Google Scholar]
  21. Simon R., Priefer U., Pühler A. 1983; Vector plasmids for in υiυo and in υitro manipulations of Gram-negative bacteria. Molecular Genetics of the Bacteria Plant Interaction98–106 Piihler A. Berlin & Heidelberg: Springer-Verlag;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-131-5-1141
Loading
/content/journal/micro/10.1099/00221287-131-5-1141
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error