1887

Abstract

Phage pilHα was specific for bacterial strains, of various genera, harbouring plasmids of the HI and HII incompatibility groups. Plaque formation was temperature sensitive in that plaques formed at 26°C but not at 37°C. Plaques were fairly clear, irregular in outline and varied from pin point to about 2 mm in diameter on all hosts where plaques were detected. The phage had an isometric hexagonal outline with a diameter of 25 nm. It contained RNA but differed from all but one other plasmid-dependent RNA phage by being sensitive to chloroform. It adsorbed along the length of the shafts of IncHI and HII plasmid-coded pili.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-131-5-1115
1985-05-01
2024-04-23
Loading full text...

Full text loading...

/deliver/fulltext/micro/131/5/mic-131-5-1115.html?itemId=/content/journal/micro/10.1099/00221287-131-5-1115&mimeType=html&fmt=ahah

References

  1. Ackermann H.-W., Audurier A., Berthiaume L., Jones L. A., Mayo J. A., Vidaver A. K. 1978; Guidelines for bacteriophage characterization. Advances in Virus Research 23:1–24
    [Google Scholar]
  2. Anderson F. S. 1975; The problem and implications of chloramphenicol resistance in the typhoid bacillus. Journal of Hygiene 74:289–299
    [Google Scholar]
  3. Anderson E. S., Smith H. R. 1972; Chloramphenicol resistance in the typhoid bacillus. British Medical Journal 3:329–331
    [Google Scholar]
  4. Anderson E. S., Humphreys G. O., Willshaw G. A. 1975; The molecular relatedness of R factors in Enterobactcria of human and animal origin. Journal of General Microbiology 91:376–382
    [Google Scholar]
  5. Bradley D. E. 1980a; Determination of pili by conjugative bacterial drug resistance plasmids of incompatibility groups B, C, H, J, K, M, V and X. Journal of Bacteriology 141:828–837
    [Google Scholar]
  6. Bradley D. E. 1980b; Morphological and serological relationships of conjugative pili. Plasmid 4:155–169
    [Google Scholar]
  7. Bradley D. E., Fleming J. 1983; Incompatibility group identification for repressed plasmids using host cell lysis by specific bacteriophages. Journal of Microbiological Methods 1:171–176
    [Google Scholar]
  8. Bradley D. E., Rutherford E. L. 1975; Basic characterization of a lipid-containing bacteriophage specific for plasmids of the P, N, and W compatibility groups. Canadian Journal of Microbiology 21:152–163
    [Google Scholar]
  9. Bradley D. E., Taylor D. E., Cohen D. R. 1980; Specification of surface mating systems among conjugative drug resistance plasmids in Escherichia coli K-12. Journal of Bacteriology 143:1466–1470
    [Google Scholar]
  10. Bradley D. E., Coetzee J. N., Bothma T., Hedges R. W. 1981a; Phage X: a plasmid-dependent, broad host range, filamentous bacterial virus. Journal of General Microbiology 126:389–396
    [Google Scholar]
  11. Bradley D. E., Coetzee J. N., Bothma T., Hedges R. W. 1981b; Phage Folac: an Folac plasmid-dependent bacteriophage. Journal of General Microbiology 126:405–411
    [Google Scholar]
  12. Bradley D. E., Coetzee J. N., Bothma T., Hedges R. W. 1981c; Phage t: a group T plasmid-dependent bacteriophage. Journal of General Micro-biology 126:397–403
    [Google Scholar]
  13. Bradley D. E., Hughes V. M., Richards H., Datta N. 1982a; R plasmids of a new incompatibility group determined constitutive production of H pili. Plasmid 7:230–238
    [Google Scholar]
  14. Bradley D. E., Sirgel F. A., Coetzee J. N., Hedges R. W., Coetzee W. F. 1982b; Phages C-2 and J: IncC and IncJ plasmid-dependent phages, respectively. Journal of General Microbiology 128:2485–2498
    [Google Scholar]
  15. Coetzee J. N. 1974; Properties of Proteus and Providence strains harbouring recombinant plasmids between P-lac and R1drd19 or R447b. Journal of General Microbiology 80:119–130
    [Google Scholar]
  16. Coetzee J. N., Lecatsas G., Coetzee W. F., Hedges R. W. 1979; Properties of R plasmid R772 and the corresponding pilus-specific phage PR772. Journal of General Microbiology 110:263–273
    [Google Scholar]
  17. Coetzee J. N., Sirgel F. A., Lecatsas G. 1980; Properties of a filamentous phage which adsorbs to pili coded by plasmids of the IncI complex. Journal of General Microbiology 117:547–551
    [Google Scholar]
  18. Coetzee J. N., Bradley D. E., Hedges R. W. 1982; Phages Iα and I2-2: IncI plasmid-dependent bacteriophages. Journal of General Microbiology 128:2797–2804
    [Google Scholar]
  19. Coetzee J. N., Bradley D. E., Hedges R. W., Fleming J., Lecatsas G. 1983; Bacteriophage M: an incompatibility group M plasmid-specific phage. Journal of General Microbiology 129:2271–2276
    [Google Scholar]
  20. Datta N. 1977; Classification of plasmids as an aid to understanding their epidemiology and evolution. Journal of Antimicrobial Chemotherapy 3:C19–23
    [Google Scholar]
  21. Datta N. 1979; Plasmid classification: incompatibility grouping. In Plasmids of Medical, Environmental and Commercial Importance3–12 Timmis K. M., Puhler A. Amsterdam: Elsevier/ North Holland Biomedical Press;
    [Google Scholar]
  22. Datta N., Hedges R. W. 1972; Host ranges of R factors. Journal of General Microbiology 70:453–460
    [Google Scholar]
  23. Datta N., Dacey S., Hughes V., Knight S., Richards H., Williams G., Casewell M., Shannon K. P. 1980; Distribution of genes for trimethoprim and gentamicin resistance in bacteria and their plasmids in a general hospital. Journal of General Microbiology 118:495–508
    [Google Scholar]
  24. Davis J. E., Strauss H. J., Sinsheimer R. L. 1961; Bacteriophage MS2: another RNA phage. Science 134:1427
    [Google Scholar]
  25. Dennison S., Hedges R. W. 1972; Host specificities of RNA phages. Journal of Hygiene 70:55–61
    [Google Scholar]
  26. Editorial 1983; Phage therapy. Lancet ii:1287–1288
    [Google Scholar]
  27. Grindley N. D. F., Grindley J. N., Anderson E. S. 1972; R factor compatibility groups. Molecular and General Genetics 119:287–297
    [Google Scholar]
  28. Hedges R. W., Jacoby G. A. 1980; Compatibility and molecular properties of plasmid Rms149 in Pseudomonas aeruginosa and Escherichia coli . Plasmid 3:1–6
    [Google Scholar]
  29. Hershey A. D., Kalmanson G., Bronfenbren-ner J. 1943; Quantitative methods in the study of the phage antiphage reaction. Journal of Immunology 46:267–276
    [Google Scholar]
  30. Jacob A. E., Shapiro J. A., Yamamoto L., Smith D. I., Cohen S. N., Berg D. 1977; Plasmid studies in Escherichia coli and other enteric bacteria. In DNA Insertion Elements, Plasmids and Episomes607–638 Bukhari A. I., Shapiro J. A., Adhya S. L. New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  31. Lawn A. M., Meynell E. 1970; Serotypes of sex pili. Journal of Hygiene 68:683–694
    [Google Scholar]
  32. Le Minor L., Coynault C., Chabbert Y., Ger-baus G., Le Minor S. 1976; Compatibility groups of metabolic plasmids. Annales de Microhiologie 127B:31–40
    [Google Scholar]
  33. Matsumoto H., Tazaki T., Hosogaya S. 1973; A generalized transducing phage of Serratia marces-cens . Japanese Journal of Microbiology 17:473–479
    [Google Scholar]
  34. Matthews R. E. F. 1982; Classification and nomenclature of viruses. Fourth report of the International Committee on Taxonomy of Viruses. Intervirology 17:1–200
    [Google Scholar]
  35. Meynell E. 1978; Experiments with sex pili: an investigation of the characters and function of F-like and I-like sex pili based on their reactions with antibody and phage. In Pili207–233 Bradley D. E., Raizen E., Fives-Taylor P., Ou J. Washington, DCInternational Conferences on Pili
    [Google Scholar]
  36. Meynell G. G., Lawn A. M. 1968; Filamentous phage specific for the I sex factor. Nature, London 217:1184–1186
    [Google Scholar]
  37. Mindich L. 1978; Bacteriophages that contain lipid. In Comprehensive Virology 12271–335 Fraenkel-Conrat C., Wagner A. M. New York: Plenum;
    [Google Scholar]
  38. Roussel A. F., Chabbert Y. A. 1978; Taxonomy and epidemiology of Gram-negative bacterial plas-mids studied by DNA-DNA filter hybridization in formamide. Journal of General Microbiology 104:269–276
    [Google Scholar]
  39. Sirgel F. A., Coetzee J. N., Hedges R. W., Lecatsas G. 1981; Phage C-I: an IncC group plasmid-specific phage. Journal of General Microbiology 122:155–160
    [Google Scholar]
  40. Smith H. R., Grindley N. D. F., Humphreys G. O., Anderson E. S. 1973; Interactions of group H resistance factors with the F factor. Journal of Bacteriology 115:623–628
    [Google Scholar]
  41. Spratt B. G., Rowbury R. H., Meynell G. G. 1973; The plasmid of Salmonella typhimurium LT2. Molecular and General Genetics 121:347–353
    [Google Scholar]
  42. Taylor D. E. 1983; Transfer-defective and tetra-cycline-sensitive mutants of the incompatibility group H1 plasmid R27 generated by insertion of transposon 7. Plasmid 9:227–239
    [Google Scholar]
  43. Taylor D. E., Grant R. B. 1976; Inhibition of bacteriophage Lambda, T1, and T7 development by R plasmids of the H incompatibility group. Anti-microbial Agents and Chemotherapy 10:762–764
    [Google Scholar]
  44. Taylor D. E., Grant R. B. 1977; R plasmids of the S incompatibility group belong to the H2 incompatibility group. Antimicrobial Agents and Chemotherapy 12:431–434
    [Google Scholar]
  45. Taylor D. E., Levine J. G. 1980; Studies of temperature-sensitive transfer and maintenance of H incompatibility group plasmids. Journal of General Microbiology 116:475–484
    [Google Scholar]
  46. Vidaver A. K., Koski R. K., van Etten J. L. 1973; Bacteriophage ϕ6: a lipid-containing virus of Pseudomonas phaseolicola . Journal of Virology 11:799–805
    [Google Scholar]
  47. Williams Smith H. 1974; Thermosensitive transfer factors in chloramphenicol-resistant strains of Sal-monella typhi . Lancet ii:281–282
    [Google Scholar]
  48. Williams Smith H., Huggins M. B. 1983; Effectiveness of phages in treating experimental Escherichia coli diarrhoea in calves, piglets and lambs. Journal of General Microbiology 129:2659–2675
    [Google Scholar]
  49. Williams Smith H., Parsell Z., Green P. 1978; Thermosensitive antibiotic resistance plasmids in Enterobacteria. Journal of General Microbiology 109:37–47
    [Google Scholar]
  50. Wolfson J. S., Hooper D. C., Swartz M. N., McHugh G. L. 1982; Antagonism of the B subunit of DNA gyrase eliminates plasmids pBR322 and pMGHO from Escherichia coli . Journal of Bacteriology 152:338–344
    [Google Scholar]
  51. Wong F. H., Bryan L. E. 1978; Characteristics of PR5, a lipid-containing plasmid-dependent phage. Canadian Journal of Microbiology 24:875–882
    [Google Scholar]
  52. Yamamoto K. R., Alberts B. M., Benzinger R., Lawhorne L., Treiber G. 1970; Rapid bacteriophage sedimentation in the presence of polyethylene glycol and its application to large-scale virus purification. Virology 40:734–744
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-131-5-1115
Loading
/content/journal/micro/10.1099/00221287-131-5-1115
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error