Subcellular Localization of -Glucanases in Ig4a Free

Abstract

Three -glucan-hydrolysing enzymes from Ig4a have been isolated. Two of them are dextranases which hydrolyse (1→6) but not (1→3) linked --glucans; one (EC 3.2.1.11,1,6---glucan 6-glucanohydrolase) is localized in the periplasm, and the other, which is an exo-enzyme (EC 3.2.1.70,1,6---glucan glucohydrolase), in the cytoplasm. The third is a mutanase (EC 3.2.1.59, 1,3-(1,3;1,4)---glucan 3-glucanohydrolase) that hydrolyses (1→3) but not (1→6) linked --glucans, and is present only in the cytoplasm.

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1985-05-01
2024-03-28
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References

  1. Caldwell R. C, Sandham H. J., Mann W. V. Jr, Finn S. B., Formicola A. J. 1971; The effect of a dextranase mouthwash on dental plaque in young adults and children. Journal of the American Dental Association 82:124–131
    [Google Scholar]
  2. Dahlqvist A. 1961; Determination of maltose and isomaltose activities with a glucose-oxidase reagent. Biochemical Journal 80:547–551
    [Google Scholar]
  3. Fitzgerald R. J., Jordan H. V. 1968; Polysacc-haride-producing bacteria and caries. Art and Science of Dental Caries Research79–86 Harris H. R. New York: Academic Press;
    [Google Scholar]
  4. Gibbons R. J., Banghart S. S. S. 1967; Synthesis of extracellular dextran by cariogenic bacteria and its presence in human dental plaque. Archives of Oral Biology 12:11–24
    [Google Scholar]
  5. Gibbons R. J., Nygaard M. 1968; Synthesis of insoluble dextran and its significance in the formation of gelatinous deposits by plaque-forming streptococci. Archives of Oral Biology 13:1249–1262
    [Google Scholar]
  6. Guggenheim B., Haller R. 1972; Purification and properties of an α-(1,3)-glucanohydrolase from Trichoderma harzianum. Journal of Dental Research 51:394–402
    [Google Scholar]
  7. Guggenheim B., Schroeder H. E. 1967; Biochemical and morphological aspects of extracellular polysaccharides produced by cariogenic streptococci. Helvetica odontologica acta 11:131–152
    [Google Scholar]
  8. Guggenheim B., Regolati B., Muhlemann H. R. 1972; Caries and plaque inhibition by mutanase in rats. Caries Research 6:289–297
    [Google Scholar]
  9. Hasegawa S., Nordin J. H., Kirkwood S. 1968; Enzymes that hydrolyze fungal cell wall polysaccharides. I. Purification and properties of an endo-α-d-(1,3)-glucanase from Trichoderma υiride. Journal of Biological Chemistry 244:5460–5470
    [Google Scholar]
  10. Heppel L. A. 1971; The concept of periplasmic membrane. Structure and Function of Biological Membranes223–247 Rothfield L. I. New York: Academic Press;
    [Google Scholar]
  11. Kelstrup J., Funder-Nielsen T. D., Møller E. N. 1973; Enzymatic reduction of the colonization of Streptococcus mutans in human dental plaque. Acta odontologica scandinavica 31:249–253
    [Google Scholar]
  12. König K. G., Guggenheim B. 1968; In υivo effects of dextranase on plaque and caries. Helvetica odontologica acta 12:48–55
    [Google Scholar]
  13. Linder L., Andersson C, Sund M.-L., Shock-man G. D. 1983; Protoplast formation and localization of enzymes in Streptococcus mitis. Infection and Immunity 40:1146–1154
    [Google Scholar]
  14. Malamy M., Horecker B. L. 1961; The localization of alkaline phosphatase in E. coli K 12. Biochemical and Biophysical Research Communications 5:104–108
    [Google Scholar]
  15. Neu H. C., Heppel L. A. 1964; The release of ribonuclease into the medium when Escherichia coli cells are converted to spheroplasts. Journal of Biological Chemistry 239:3893–3900
    [Google Scholar]
  16. Neu H. C., Heppel L. A. 1965; The release of enzymes from Escherichia coli by osmotic shock and during the formation of spheroplasts. Journal of Biological Chemistry 240:3685–3692
    [Google Scholar]
  17. Newbrun E. 1976; Polysaccharide synthesis in plaque. Microbial Aspects of Dental Caries649–664 Stiles H. M., Loesche W. J., O’Brien T. C. Washington DC:: Information Retrieval;
    [Google Scholar]
  18. Nossal N. G., Heppel L. A. 1966; The release of enzymes by osmotic shock from Escherichia coli in exponential phase. Journal of Biological Chemistry 241:3055–3062
    [Google Scholar]
  19. Nyman S., Lindhe J., Janson J. 1972; The effect of bacterial dextranase on human dental plaque formation and gingivitis development. Odontologisk revy 23:243–252
    [Google Scholar]
  20. Simonson L. G., Lamberts B. L., Reiher D. A., Walter R. G. 1983; Prevention of dental caries in hamsters by an endo-1,3-α-d-glucanase. Journal of Dental Research 62:395–397
    [Google Scholar]
  21. Somogyi M. 1952; Notes on sugar determination. Journal of Biological Chemistry 195:19–23
    [Google Scholar]
  22. Staat R. H., Schachtele C. H. 1976; Analysis of the dextranase activity produced by an oral strain of Bacteroides ochraceus. Journal of Dental Research 55:1103–1110
    [Google Scholar]
  23. Staat R. H., Langley S. D., Swenson J. I. 1982; In υivo relationships of the dextran-degrading oral microbiota to Streptococcus mutans and caries experience. Caries Research 16:18–25
    [Google Scholar]
  24. Takahashi N. 1982; Isolation and properties of dextranases from Bacteroides oralis Ig4a. Microbiology and Immunology 26:375–386
    [Google Scholar]
  25. Takahashi N., Horikawa T., Mizuno F., Yama-moto A., Takamori K. 1982; Effect of glucanases of Bacteroides oralis Ig4a on artificial plaque of Streptococcus mutans. Japanese Journal of Oral Biology 24:1058–1060
    [Google Scholar]
  26. Walker G. J., Pulkownik A., Morrey-Jones J. G. 1981; Metabolism of the polysaccharides of human dental plaque: release of dextranase in batch cultures of Streptococcus mutans. Journal of General Microbiology 127:201–208
    [Google Scholar]
  27. Zipser D. 1963; A study of the urea-produced subunits of β-galactosidase. Journal of Molecular Biology 7:113–121
    [Google Scholar]
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