1887

Abstract

A nucleic acid spot hybridization assay was used to detect DNA. The hybridization probes included DNA isolated from elementary bodies of lymphogranuloma venereum (LGV) strains and cloned fragments of both chromosomal and plasmid DNA.The sensitivity of the test was in the range 10 to 100 pg homologous DNA and 10 infected cells. Cross-reactivity with bacterial DNA was avoided when purified chlamydia-specific DNA fragments were used as probes. . was detectable in most of the clinical specimens with large amounts of infectious particles. Also some isolation-negative specimens gave a positive signal in the test.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-131-4-975
1985-04-01
2024-04-20
Loading full text...

Full text loading...

/deliver/fulltext/micro/131/4/mic-131-4-975.html?itemId=/content/journal/micro/10.1099/00221287-131-4-975&mimeType=html&fmt=ahah

References

  1. Darougar S., Treharne J. D., Dwyer R., St C., Kinnison J. R., Jones B. R. 1971; Isolation of TRIC agent (Chlamydia) in irradiated McCoy cell culture from endemic trachoma in field studies in Iran. British Journal of Ophthalmology 55:591–599
    [Google Scholar]
  2. Dretzen G., Bellard M., Sassone-Corsi P., Chambon P. 1981; A reliable method for the recovery of DNA fragments from agarose and acrylamide gels. Analytical Biochemistry 112:295–298
    [Google Scholar]
  3. Hyypiä T., Larsen S. H., Ståhlberg T., Terho P. 1984; Analysis and detection of chlamydial DNA. Journal of General Microbiology 131:3159–3164
    [Google Scholar]
  4. Lennette E. H., Balows A., Hausler W. J. Jr, Truant J. P. 1980 Manual of Clinical Microbiology, 3. Washington DC: American Society for Microbiology;
    [Google Scholar]
  5. Palva A., Jousimies-Somer H., Saikku P., Väänänen P., Soderlund H., Ranki M. 1984; Detection of Chlamydia trachomatis by nucleic acid sandwich hybridization. FEMS Microbiology Letters 23:83–89
    [Google Scholar]
  6. Rigby P. W. J., Diecmann M., Rhodes C., Berg P. 1977; Labelling deoxyribonucleic acid to high specific activity by nick translation with DNA-polymerase I. Journal of Molecular Biology 113:237–251
    [Google Scholar]
  7. Tam M. R., Stamm W. E., Handsfield H. H., Stephens R., Kuo C.-C., Holmes K. K., Ditzen-Berger K., Krieger M., Nowinski R. C. 1984; Culture-independent diagnosis of Chlamydia trachomatis using monoclonal antibodies. New England Journal of Medicine 310:1146–1150
    [Google Scholar]
  8. Terho P. 1978; Isolation techniques of Chlamydia trachomatis from patients with nonspecific ureth-ritis. Dermatologishe Monatsschrift 164:515–520
    [Google Scholar]
  9. Totten P. A., Holmes K. K., Handsfield H. H., Knapp J. S., Perine P. L., Falkow S. 1983; DNA hybridization technique for the detection of Neisseria gonorrhoeae in men with urethritis. Journal of Infectious Diseases 148:462–471
    [Google Scholar]
  10. Wahl G. M., Stern M., Stark G. R. 1979; Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate. Proceedings of the National Academy of Sciences of the United States of America 763683–3687
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-131-4-975
Loading
/content/journal/micro/10.1099/00221287-131-4-975
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error