RNA synthesis was followed during amino acid starvation of strains of Escherichia coli that contained both the relaxed (relA) mutation and a mutation affecting ribosome assembly that results in oversynthesis of RNA. The ribosome mutation did not by itself lead to relaxedness. The relaxed mutation could be expressed in organisms that contained the ribosome mutation.
BlundellM. R.,
WildD. G.1969; Inhibition of bacterial growth by metal salts. The accumulation of ribonucleic acid during inhibition of Escherichia coli by cobalt chloride. Biochemical Journal 115:213–223
ButlerP. D.,
WildD. G.1985; The location of a mutation affecting ribosomal protein synthesis by Escherichia coli
. Journal of General Microbiology 131:135–144
ButlerP. D.,
SimsP. F. G.,
WildD. G.1978; Binding of chloramphenicol and a fragment of aminoacyl-transfer ribonucleic acid to ribosomes and a ribosome precursor from a mutant of Escherichia coli
. Biochemical Journal 172:503–508
GausingK.1980; Regulation of ribosome biosynthesis in E. coli
. In Ribosomes: Structure, Function and Genetics693–718ChamblissG.,
CravenG. R.,
DaviesJ.,
DavisK.,
KalianL.,
NomuraM.
Baltimore: University Park Press;
Jinks-RobertsonS.,
GourseR. L.,
NomuraM.1983; Expression of rRNA and tRNA genes in Escherichia coli: evidence for feedback regulation by products of rRNA operons. Cell 33:865–876
KajitaniM.,
IshihamaA.1984; Promoter selectivity of Escherichia coli RNA polymerase. Differential stringent control of the multiple promoters from ribosomal RNA and protein operons. Journal of Biological Chemistry 259:1951–1957
MacDonaldR. E.,
TurnockG.,
ForchhammerJ.1967; The synthesis and function of ribosomes in a new mutant of Escherichia coli
. Proceedings of the National Academy of Sciences of the United States of America57141–147
MarkeyF.,
SimsP. F. G.,
WildD. G.1976; The composition of an unusual precursor of 50S ribosomes in a mutant of Escherichia coli
. Biochemical Journal 158:451–456
SykesJ.,
MetcalfE.,
PickeringJ. D.1977; The nature of the proteins present in the ‘relaxed particles’ from methionine-starved Escherichia coli A19 (Hfr rel met rns). Journal of General Microbiology 98:17–27