1887

Abstract

Examination of a series of isolates of collected over an 18 month period from a chronic-care patient at Bristol Royal Infirmary revealed the emergence of resistance to carbenicillin. Resistence was mediated by a 47 kb plasmid which transferred by conjugation to a plasmid-free strain of but not to Carbenicillin-sensitive isolates were either plasmid free or contained a 36 kb cryptic plasmid. Restriction endonuclease mapping of this plasmid showed it to be closely related to 32 kb and 34 kb cryptic plasmids reported previously in from Bristol. Mapping of the R plasmid showed it to be derived from the 34 kb cryptic plasmid by transposition of two copies of Tn.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-131-4-927
1985-04-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/micro/131/4/mic-131-4-927.html?itemId=/content/journal/micro/10.1099/00221287-131-4-927&mimeType=html&fmt=ahah

References

  1. Bennett P. M., Richmond M. H. 1976; Translo-cation of a discrete piece of deoxyribonucleic acid carrying an amp gene between replicons in Escheri-chia coli . Journal of Bacteriology 126:1–6
    [Google Scholar]
  2. Bennett P. M., Richmond M. H., Petrocheilou V. 1980; The inactivation of tet genes on a plasmid by duplication of one inverted repeat of a transposon like structure which itself mediates tetracycline resistance. Plasmid 3:135–149
    [Google Scholar]
  3. Bidwell J. L., Reeves D. S. 1980; Isoelectric focusing of β-Mactamases. Journal of Antimicrobial Chemotherapy 6:793
    [Google Scholar]
  4. Bradley D. E., Taylor D. E., Cohen D. R. 1980; Specification of surface mating systems among conjugative drug resistance plasmids in Escherichia coli K-12. Journal of Bacteriology 143:1466–1470
    [Google Scholar]
  5. Casse F., Boucher C, Julliot J. S., Michel M., Denarie J. 1979; Identification and characterization of large plasmids in Rhizobium meliloti using agarose gel electrophoresis. Journal of General Microbiology 113:229–242
    [Google Scholar]
  6. Datta N., Hedges R. W. 1972; Trimethoprim resistance conferred by plasmids in Enterobacteria-ceae . Journal of General Microbiology 72:349–356
    [Google Scholar]
  7. Dobritsa A. P., Ksenzenko V. N., Fedoseeva V. B., Alexandrov A. A., Kamynina T. P., Khmelnitsky M. I. 1980; Isolation of transposon TnA from plasmid RP4 carrying two copies of this element. Gene 8:153–162
    [Google Scholar]
  8. Dobritsa A. P., Ivanova Z. A., Fedoseeva V. A. 1983; Transposition of DNA fragments flanked by two inverted Tn1 sequences: translocation of the plasmid RP4::Tn1 region harboring the TcR marker. Gene 22:237–243
    [Google Scholar]
  9. Hansen J. B., Olsen R. H. 1978; Isolation of large bacterial plasmids and characterization of the P2 incompatibility group plasmids pMGl and pMG5. Journal of Bacteriology 135:227–238
    [Google Scholar]
  10. Hawkey P. M. 1984; Providencia stuartii: a review of a multiply antibiotic resistant bacterium. Journal of Antimicrobial Chemotherapy 13:209–226
    [Google Scholar]
  11. Hawkey P. M, Bennett P. M., Hawkey C. A. 1984; Cryptic plasmids in hospital isolates of Providencia stuarti . Journal of Medical Microbiology 18:277–284
    [Google Scholar]
  12. Heffron F., Rubens C., Falkow S. 1977; Transposition of a plasmid DNA sequence which mediates ampicillin resistance: general description and epidemiological considerations. In DNA Insertion Elements, Plasmids, and Episomes151–160 Bukhari A. I., Shapiro J. A., Adhya S. L. Cold Spring Harbor, NY:: Cold Spring Harbor Laboratory;
    [Google Scholar]
  13. Matthew M. 1979; Plasmid-mediated β-Mactamases of Gram-negative bacteria: properties and distribution. Journal of Antimicrobial Chemotherapy 5:349–358
    [Google Scholar]
  14. Matthew M., Harris A. M., MARshall M. J., Ross G. W. 1975; The use of analytical isoelectric focusing for detection and identification of β-lactamases. Journal of General Microbiology 88:169–178
    [Google Scholar]
  15. Mchale P. J., Keane C. T., Dougan G. 1981; Antibiotic resistance in Providencia stuartii isolated in hospitals. Journal of Clinical Microbiology 13:1099–1104
    [Google Scholar]
  16. O'Brien T. F., Ross D. G., Guzman M. A., Medeiros A. A., Hedges R. W., Botstein D. 1980; Dissemination of an antibiotic resistance plasmid in hospital patient flora. Antimicrobial Agents and Chemotherapy 17:537–543
    [Google Scholar]
  17. Roberts M., Elwell L. P., Falkow S. 1977; Molecular Characterization of two beta-lactamasespecifying plasmids isolated from Neisseria gonorrhoeae . Journal of Bacteriology 131:557–563
    [Google Scholar]
  18. Robinson M. K., Bennett P. M., Richmond M. H. 1977; Inhibition of TnA translocation by TnA. Journal of Bacteriology 129:407–414
    [Google Scholar]
  19. Robinson M. K., Bennett P. M., Grinsted J., Richmond M. H. 1978; The stable carriage of two TnA units on a single replicon. Molecular and General Genetics 160:339–346
    [Google Scholar]
  20. Rubens C. E., Farrar W. E., Mcgee Z. A., Schaffner W. 1981; Evolution of a plasmid mediating resistance to multiple antimicrobial agents during a prolonged epidemic of nosocomial infections. Journal of Infectious Diseases 143:170–181
    [Google Scholar]
  21. Sanchez J., Bennett P. M., Richmond M. H. 1982; Expression of elt-B, the gene encoding the B subunit of the heat-labile enterotoxin of Escherichia coli, when cloned in pACYC184. FEMS Microbiology Letters 14:1–5
    [Google Scholar]
  22. Tompkins L. S., Plorde J. J., Falkow S. 1980; Molecular analysis of R-factors from multiresistant nosocomial isolates. Journal of Infectious Diseases 141:625–636
    [Google Scholar]
  23. Wallace L. J., Ward J. M., Richmond M. H. 1981; The tnpR gene product of TnA is required for transposition immunity. Molecular and General Genetics 184:87–91
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-131-4-927
Loading
/content/journal/micro/10.1099/00221287-131-4-927
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error