1887

Abstract

The generalized transducing phage Pf 16h2 has been used to confirm linkage relationships of chromosomal markers of previously determined from their time-of-entry in Hfr crosses, and to map new auxotrophic mutations. By means of spot matings using Hfr donors of known origin of transfer, catabolic markers forming part of a closely linked group of operons referred to as a superoperonic cluster have been shown to be chromosomally located and their map positions determined. R-prime-mediated interspecific complementation has been used to equate functionally 21 auxotrophic loci in . and . and the distribution of these loci on the two genetic maps has been compared. While both maps reveal that auxotrophic markers are largely restricted to about 40% of the chromosome and that auxotrophic markers of similar phenotype are not clustered, there is evidence of at least seven chromosomal rearrangements since divergence from a presumed common ancestor.

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1985-04-01
2021-08-02
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References

  1. Adams M. H. 1959 Bacteriophages New York: Interscience;
    [Google Scholar]
  2. Bachmann B. J. 1983; Linkage map of Escherichia coli K-12. , 7. Microbiological Reviews 47:180–230
    [Google Scholar]
  3. Benedik M., Fennewald M., Shapiro J. 1977; Transposition of a beta-lactamase locus from RP1 into Pseudomonas putida degradative plasmids. Journal of Bacteriology 129:809–814
    [Google Scholar]
  4. Campbell A. 1981; Evolutionary significance of accessory DNA elements in bacteria. Annual Review of Microbiology 35:55–83
    [Google Scholar]
  5. Chakrabarty A. M., Gunsalus C. F., Gunsalus I. C. 1968; Transduction and clustering of genes in fluorescent pseudomonads. Proceedings of the National Academy of Sciences of the United States of America 60168–175
    [Google Scholar]
  6. Clarke P. H., Ornston L. N. 1975; Metabolic pathways and regulation - part II. In Genetics and Biochemistry of Pseudomonaspp. 263–340 Clarke P. H., Richmond M. H. London: Wiley.;
    [Google Scholar]
  7. Dean H. F., Morgan A. F. 1983; Integration of R91-5::Tn30/ into the Pseudomonas putida PPN chromosome and genetic circularity of the chromosomal map. Journal of Bacteriology 153:485–497
    [Google Scholar]
  8. Gillis M., De Ley J., De Cleene M. 1970; The determination of molecular weight of bacterial genome DNA from renaturation rates. European Journal of Biochemistry 12:143–153
    [Google Scholar]
  9. Haas D. 1983; Genetic aspects of biodegradation by pseudomonads. Experientia 39:1199–1213
    [Google Scholar]
  10. Holloway B. W. 1978; Isolation and characterisation of an R′ plasmid in Pseudomonas aeruginosa . Journal of Bacteriology 133:1078–1082
    [Google Scholar]
  11. Holloway B. W., Matsumoto H. 1984; Genetic map of Pseudomonas aeruginosa PAO. Genetic Maps 3:194–197
    [Google Scholar]
  12. Holloway B. W., Krishnapillai V., Morgan A. F. 1979; Chromosomal genetics of Pseudomonas . Microbiological Reviews 43:73–102
    [Google Scholar]
  13. Krishnapillai V. 1971; A novel transducing phage; its role in recognition of a possible new host-controlled modification system in Pseudomonas aeruginosa . Molecular and General Genetics 114:134–143
    [Google Scholar]
  14. Leisinger T., Haas D., Hegarty M. P. 1972; Indospicine as an arginine antagonist in Escherichia coli and Pseudomonas aeruginosa . Biochimica et biophysica acta 161:214–219
    [Google Scholar]
  15. Morgan A. F. 1979; Transduction of Pseudomonas aeruginosa with a mutant of bacteriophage. , 79. Journal of Bacteriology 139:137–140
    [Google Scholar]
  16. Morgan A. F. 1982; Isolation and characterisation of Pseudomonas aeruginosa R′ plasmids constructed by interspecific mating. Journal of Bacteriology 149:654–661
    [Google Scholar]
  17. Mylroie J. R., Friello D. A., Siemens T. V., Chakrabarty A. M. 1978; Mapping of Pseudomonas putida chromosomal genes with a recombin-ant sex-factor plasmid. Molecular and General Genetics 157:231–237
    [Google Scholar]
  18. Palleroni N. J. 1983; The taxonomy of bacteria. Bioscience 33:370–377
    [Google Scholar]
  19. Parke D., Ornston L. N. 1976; Constitutive synthesis of enzymes of the protocatechuate pathway and of the β-ketoadipate uptake system in mutant strains of Pseudomonas putida . Journal of Bacteriology 126:272–281
    [Google Scholar]
  20. Riess G., Holloway B. W., Puhler A. 1980; R68.45, a plasmid with chromosome mobilising ability (Cma), carries a tandem duplication. Genetical Research 36:105–115
    [Google Scholar]
  21. Riley M., Anilionis A. 1978; Evolution of the bacterial genome. Annual Review of Microbiology 32:519–560
    [Google Scholar]
  22. Royle P. L., Matsumoto H., Holloway B. W. 1981; Genetic circularity of the Pseudomonas aeruginosa PAO chromosome. Journal of Bacteriology 145:145–155
    [Google Scholar]
  23. Stanier R. Y., Palleroni N. J., Doudoroff M. 1966; The aerobic pseudomonads: a taxonomic study. Journal of General Microbiology 43:159–271
    [Google Scholar]
  24. Stanisich V. A., Holloway B. W. 1972; A mutant sex factor of Pseudomonas aeruginosa . Geneti-cal Research 19:91–108
    [Google Scholar]
  25. Starlinger P. 1977; DNA rearrangements in procaryotes. Annual Review of Genetics. 11:103–126
    [Google Scholar]
  26. Thayer J. R., Wheelis M. L. 1976; Characterisation of a benzoate permease mutant of Pseudomonas putida . Archives of Microbiology 110:37–42
    [Google Scholar]
  27. Watson J. M., Holloway B. W. 1978; Chromosome mapping in Pseudomonas aeruginosa PAT. Journal of Bacteriology 133:1113–1125
    [Google Scholar]
  28. Watterson G. A., Ewans W. J., Hall T. E., Morgan A. F. 1982; The chromosome inversion problem. Journal of Theoretical Biology 99:1–7
    [Google Scholar]
  29. Wheelis M. L. 1975; The genetics of dissimilatory pathways in Pseudomonas . Annual Review of Microbiology 29:505–524
    [Google Scholar]
  30. Wheelis M. L., Ornston L. N. 1972; Genetic control of enzyme induction in the β-ketoadipate pathway of Pseudomonas putida: deletion mapping of cat mutations. Journal of Bacteriology 109:790–795
    [Google Scholar]
  31. Wheelis M. L., Stanier R. Y. 1970; The genetic control of dissimilatory pathways in Pseudomonas putida . Genetics 66:245–266
    [Google Scholar]
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