SUMMARY: NCIB 8250 was grown in batch culture on 46 separate sources of carbon + energy and the molar growth yields were measured. The organism was also grown in continuous culture on L-mandelate, phenylglyoxylate and succinate and the results used to calculate maximum molar growth yields. The elemental composition of the bacteria was determined. Growth equations for each substrate were constructed so that the amount of oxygen consumed per mol of substrate could be calculated. Growth yields on oxygen (o) were then estimated. The o values were low [averaging about 19 g dry wt (mol O ]. The most likely explanation for the low yields is that the effective P/O ratio is only about one, although there are probably at least two sites of oxidative phosphorylation.


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