1887

Abstract

H production by the human protozoan parasite was monitored continuously under a mobile gas phase using a membrane-inlet mass spectrometer. Simultaneous and continuous measurement of dissolved H, O and CO indicated that H evolution was inhibited at levels of O (<0‧25 μ) undetectable by the technique, whereas CO production was stimulated. Respiration was not stimulated by admitting H to the gas phase. Metronidazole inhibited both H and CO production. Values of for inhibition of H formation in strain ATCC 30001 (metronidazole sensitive) of 0‧16 m and in strain 85 (metronizadole resistant) of 1‧0 m were obtained. These data suggest that metronidazole not only competes with protons as electron acceptor but that the drug itself or a product of reduction actively inhibits some hydrogenosomal enzyme or electron carrier involved in H production. Under these conditions metronidazole inhibition leads to irreversible loss of cell motility.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-131-4-849
1985-04-01
2021-08-05
Loading full text...

Full text loading...

/deliver/fulltext/micro/131/4/mic-131-4-849.html?itemId=/content/journal/micro/10.1099/00221287-131-4-849&mimeType=html&fmt=ahah

References

  1. Breccia A., Cavalleri B., Adams G.E. 1982; Nitroimidazoles: Chemistry, Pharmacology and Clinical Applications. New York: Plenum;
    [Google Scholar]
  2. Coombs G.H. 1976; Studies on the activity of nitroimidazoles. Biochemistry of Parasites and Host-Parasite Relationships545–552 Van den Bossche H. Amsterdam: North Holland;
    [Google Scholar]
  3. Degn H., Lundsgaard J.S., Petersen L.C., Ormicki A. 1980; Polarographic measurement of steady state kinetics of oxygen uptake by biochemical samples. Methods of Biochemical Analysis 26:47–77
    [Google Scholar]
  4. Diamond L. 1957; The establishment of various trichomonads of animals and man in axenic cultures. Journal of Parasitology 43:488–490
    [Google Scholar]
  5. Edwards D.I., Dye M., Carne H. 1973; The selective toxicity of antimicrobial nitroheterocycle drugs. Journal of General Microbiology 76:135–145
    [Google Scholar]
  6. Lindmark D.G., Müller M. 1973; Hydrogeno-some, a cytoplasmic organdie of the anaerobic flagellate Tritrichomonas foetus, and its role in pyruvate metabolism. Journal of Biological Chemistry 248:7724–7728
    [Google Scholar]
  7. Lloyd D., Pedersen J.Z. 1985; Metronidazole radical anion generation in vivo in Trichomonas vaginalis: O2 quenching is enhanced in a drug-resistant strain. Journal of General Microbiology 131:87–92
    [Google Scholar]
  8. Lloyd D., Scott R.I. 1983; Direct measurement of dissolved gases in microbiological systems using membrane inlet mass spectrometry. Journal of Microbiological Methods 1:313–328
    [Google Scholar]
  9. Lundsgaard J.S., Petersen L.C., Degn H. 1976; Mass spectrometric determination of oxygen kinetics in biochemical systems. Measurements of Oxygen168–183 Edited by Degn H., Balslev I., Brook R. Amsterdam: Elsevier;
    [Google Scholar]
  10. Meingassner J.G., Meith H., Czok R., Lindmark D.G., Müller M. 1978; Assay conditions and the demonstration of nitroimidazole resistance in Tritrichomonas foetus . Antimicrobial Agents and Chemotherapy 13:1–13
    [Google Scholar]
  11. Müller M. 1983; Mode of action of metronidazole on anaerobic bacteria and protozoa. Surgery 93:165–171
    [Google Scholar]
  12. Müller M., Meingassner J.G., Miller W.A., Ledger W.J. 1980; Three metronidazole-resistant strains of Trichomonas vaginalis from the United States. American Journal of Obstetrics and Gynecology 138:808–812
    [Google Scholar]
  13. O’brian R.W., Morris J.G. 1972; Effect of metronidazole on hydrogen production by Clostridium acetobutylicum . Archives of Microbiology 84:225–233
    [Google Scholar]
  14. Ohnishi T., Lloyd D., Lindmark D.G., Müller M. 1980; Respiration of Trichomonas foetus: components detected in hydrogenosomes and in intact cells by electron paramagnetic resonance spectrometry. Molecular and Biochemical Parasitology 2:39–50
    [Google Scholar]
  15. Wardman P., Clarke E.D. 1976; Oxygen inhibition of nitroreductase: electron transfer from nitro radical-anions to oxygen. Biochemical and Biophysical Research Communications 69:942–949
    [Google Scholar]
  16. Wilhelm E., Battino R., Wilcock R.J. 1977; Low pressure solubility of gases in liquid water. Chemical Reviews 77:226–250
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-131-4-849
Loading
/content/journal/micro/10.1099/00221287-131-4-849
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error