1887

Abstract

The reaction components and conditions affecting CAMP factor () induced lysis of target cells have been investigated. Both the amount of sphingomyelinase used and the time of preincubation with sphingomyelinase directly affected the rate of haemolysis by CAMP factor. The CAMP factor induced lysis was temperature dependent between 15 and 30 °C and was proportional to the amount of CAMP factor added.

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/content/journal/micro/10.1099/00221287-131-4-817
1985-04-01
2024-11-14
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References

  1. Bernheimer A. W., Avigad L. S., Kim K. S. 1974; Staphylococcal sphingomyelinase (β-hemo-lysin). Annals of the New York Academy of Sciences 236:292–306
    [Google Scholar]
  2. Bernheimer A. W., Linder R., Avigad L. S. 1979; Nature and mechanism of action of the CAMP protein of group B streptococci. Infection and Immunity 23:838–844
    [Google Scholar]
  3. Brown J., Gray E. D., Wannamaker L. W. 1972; CAMP factor of group B streptococci. Abstracts of the Annual Meeting of the American Society for Microbiology M145:104
    [Google Scholar]
  4. Brown J., Farnsworth R., Wannamaker L. W., Johnson D. W. 1974; CAMP factor of group B streptococci: production, assay, and neutralization by sera from immunized rabbits and experimental infected cows. Infection and Immunity 9:377–383
    [Google Scholar]
  5. Christie R., Atkins N. E., Munch-Petersen E. 1944; A note on a lytic phenomenon shown by group B streptococci. Australian Journal of Experimental Biology and Medical Science 22:197–200
    [Google Scholar]
  6. Colley C. M., Zwaal R. F. A., Roelofsen B., Van Deenen L. L. M. 1973; Lytic and non-lytic degradation of phospholipids in mammalian ery-throcytes by pure phospholipase. Biochimica et biophysica acta 307:74–82
    [Google Scholar]
  7. Doery H. M., Magnusson B. J., Cheyne I. M., Gulasek-Haram J. 1963; A phospholipase in staphylococcal toxin which hydrolyses sphingomye-lin. Nature, London 198:1091–1092
    [Google Scholar]
  8. Esseveld H., Daniels-Bosman M. S. M., Leijnse B. 1958; Some observations about the CAMP reaction and its application to human β-haemolytic streptococci. Antonie van Leeuwenhoek 24:145–156
    [Google Scholar]
  9. Esseveld H., Monnier-Goudzwaard C., Van Eijk H. G., Van Soestbergen M. G. 1975; Chemical nature of a substance isolated from a group B streptococcus causing the ‘CAMP’ reaction. Antonie van Leeuwenhoek 41:449–454
    [Google Scholar]
  10. Fehrenbach F. J., Schmidt C.-M., Sterzik B., Jurgens D. 1984; Interaction of amphiphilic bacterial polypeptides with artificial membranes. In Bacterial Protein Toxins317–324 Alouf J. E., Fehrenbach F. J., Freer J. H., Jeljaszewicz J. London: Academic Press;
    [Google Scholar]
  11. Huser H., Goeke L., Karst G., Fehrenbach F. J. 1983; Fermenter growth of Streptococcus agalactiae and large-scale purification of CAMP factor. Journal of General Microbiology 129:1295–1300
    [Google Scholar]
  12. Ikezawa H., Mori M., Ohyabu T., Taguchi R. 1978; Studies on sphingomyelinase of Bacillus cereus. I. Purification and properties. Biochimica et biophysica acta 528:247–256
    [Google Scholar]
  13. Juergens D., Huser H., Fehrenbach F. J. 1984; Purification and characterization of CAMP-factor from Streptococcus agalactiae by hydrophobic interaction chromatography and chromatofocusing. In Bacterial Protein Toxins245–246 Alouf J. E., Fehrenbach F. J., Freer J. H., Jeljaszewicz J. London: Academic Press;
    [Google Scholar]
  14. Krug E. L., Truesdale N. J., Kent C. 1979; A simplified assay for phospholipase C. Analytical Biochemistry 97:43–47
    [Google Scholar]
  15. Montfort A., Boere W. A. M. 1978; Cholesterol and phospholipid composition of erythroblasts isolated from mouse spleen after Rauscher leukemia virus infection. Lipids 13:580–587
    [Google Scholar]
  16. Nelson G. J. 1967; Lipid composition of erythro-cytes in various mammalian species. Biochimica et biophysica acta 144:221–232
    [Google Scholar]
  17. Rogolsky M. 1979; Nonenteric toxins of Staphylo-coccus aureus. Microbiological Reviews 43:320–360
    [Google Scholar]
  18. Ways P., Hanahan D. J. 1964; Characterization and quantification of red cell lipids in normal man. Journal of Lipid Research 5:318–328
    [Google Scholar]
  19. Wiseman G. M., Caird J. D. 1967; The nature of staphylococcal beta hemolysin. I. Mode of action. Canadian Journal of Microbiology 13:369–376
    [Google Scholar]
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