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Volume 131, Issue 4

Mechanism of Action of Nikkomycin and the Peptide Transport System of Free

Abstract

Nikkomycin was found to be a potent growth inhibitor of through competitive inhibition of chitin synthase [ = 0·16 μ (0·1 μg ml)]. The activity of the peptide-nucleoside drug was antagonized by both peptone and defined peptides. Transported dipeptides were effective antagonists while transported oligopeptides were not. A mutant of resistant to the effects of nikkomycin through a transport defect was unable to transport dipeptides, while oligopeptide uptake was apparently unaffected. At least two peptide permeases are operational in this organism.

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© Society for General Microbiology 1985
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1985-04-01
2025-07-08
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References

  1. Dahn U., Hagenmaier H., Konig W. A., Wolf G., Zahner H. 1976; Stoffwechselprodukte von mikroorganismen 154 Mitteilung. Nikkomycin ein neuer Hemmstoff der Chitinsynthase bei Pilzen. Archives of Microbiology 197:143–160
     [Google Scholar]
  2. Davies M. D. 1980; Peptide uptake in Candida albicans . Journal of General Microbiology 121:181–186
     [Google Scholar]
  3. Dixon M. 1953; The determination of enzyme inhibition constants. Biochemical Journal 55:170–175
     [Google Scholar]
  4. Hori M., Kakiki K., Misato T. 1977; Antagonistic effect of dipeptides of the uptake of polyoxin A by Alternaria kikuchiana . Journal of Pesticide Science 2:139–149
     [Google Scholar]
  5. Kingsbury W. R., Boehm J. C., Mehta R. J., Grappel S. F. 1983; Transport of antimicrobial agents using peptide carrier systems: anticandidal activity of m-fiuorophenylalanyl-peptide conjugates. Journal of Medicinal Chemistry 26:1725–1729
     [Google Scholar]
  6. Logan D. A., Becker J. M., Naider F. 1979; Peptide transport in Candida albicans. Journal of General Microbiology 114:179–186
     [Google Scholar]
  7. Macgruder G. C. 1979; The chitin synthetase enzyme of Schizophyllum commune ‘wild type’ and the mutant ‘streak’. PhD dissertation University of Missouri, Rolla, Mo.; USA:
     [Google Scholar]
  8. Marder R., Rose B., Becker J. M., Naider F. 1978; Isolation of a peptide transport-deficient mutant of yeast. Journal of Bacteriology 136:1174–1177
     [Google Scholar]
  9. Nisbet T. M., Payne J. W. 1979a; Specificity of peptide uptake in Saccharomyces cerevisiae and isolation of a bacilysin-resistant, peptide transport deficient mutant. FEMS Letters 7:193–196
     [Google Scholar]
  10. Nisbet T. M., Payne J. W. 1979b; Peptide uptake in Saccharomyces cerevisiae Characteristics of a transport system shared by dipeptides and oligo-peptides. Journal of General Microbiology 115:127–133
     [Google Scholar]
  11. Ryder N. S., Peberdy J. F. 1977; Chitin synthase in Aspergillus nidulans : Properties and proteolytic activation. Journal of General Microbiology 99:69–76
     [Google Scholar]
  12. Sarthou P., Gonneau M., Le Goffic F. 1983; Photoaffinity inhibition of peptide transport in yeast. Biochemical and Biophysical Research Communications 110:884–889
     [Google Scholar]
/content/journal/micro/10.1099/00221287-131-4-775
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Mechanism of Action of Nikkomycin and the Peptide Transport System of Candida albicans
Microbiology 131, 775 (1985); https://doi.org/10.1099/00221287-131-4-775
/content/journal/micro/10.1099/00221287-131-4-775
/content/journal/micro/10.1099/00221287-131-4-775
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