SUMMARY: During continuous cultivation of and two different strains of mean cell volume was found to be directly proportional to the agitation rate of the fermenter. For any one piece of equipment and microbial strain, highly reproducible linear relationships were measured, with similar intercepts and gradients for aerobic and anaerobic cultures independent of overall medium concentration, gradients for nitrogen-limited culture being always greater than those for carbon-limited culture. Alternative fermenter configurations or use of a different microbial strain furnished similar linear relationships, but slope and intercept were changed, indicating a dependence both on the geometry of the fermentation system and also on the microbial strain.0


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