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The addition of tribromo-, tetrabromo- and chloromethanes to cultures of Aspergillus parasiticus strongly stimulated aflatoxin biosynthesis. The presence of phenobarbital in the cultures enhanced this effect. Following the addition of 14CC14 to a culture, after 15 d incubation, 8·6% of the total radioactivity was recovered from the mycelium, and 4·7% from the culture filtrate. Some 0·4% of the total radioactivity was found in microsomes, indicating their involvement in CC14 activation and biotransformation.
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