1887

Abstract

Nutritional shifts up of synchronized or random populations of (strain ML30 and derivatives of B/r and K12) to a richer medium were followed (nutritional pulse) or not (shift-up) by shifts down to the original poor medium. In most cases, the first postshift division was clearly postponed by a shift-up or a nutritional pulse. This delay of the first division was almost constant, whatever the cell age at the time of the transition, and, for the nutritional pulse only, whatever the time spent by cells in the rich medium (between 2 and 25% of the generation time characteristic of poor medium). Following a shift-up, the new steady state of division rhythm and of mean cell mass was reached at about the second postshift division, whereas after a nutritional pulse, it took three generations to return to the steady state prevailing in poor medium. When both the poor and the rich medium were varied, the extent of the postponement of cell division after a nutritional pulse increased when the amplitude of the stimulus (i.e. the difference in richness) was increased. This was not the case with a simple shift-up, where it seemed that the postponement was compensated in part by the accelerating effects of the rich medium.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-131-3-677
1985-03-01
2021-10-25
Loading full text...

Full text loading...

/deliver/fulltext/micro/131/3/mic-131-3-677.html?itemId=/content/journal/micro/10.1099/00221287-131-3-677&mimeType=html&fmt=ahah

References

  1. Clark D. J. 1968; The regulation of DNA replication and cell division in Escherichia coli B/r. Cold Spring Harbor Symposia on Quantitative Biology 33:823–838
    [Google Scholar]
  2. Cooper S. 1969; Cell division and DNA replication following a shift to a richer medium. Journal of Molecular Biology 43:1–11
    [Google Scholar]
  3. Cooper S., Helmstetter C. E. 1968; Chromosome replication and the division cycle of Escherichia coli B/r. Journal of Molecular Biology 31:519–540
    [Google Scholar]
  4. Helmstetter C. E., Cooper S., Pierucci O., Revelas E. 1968; On the bacterial life sequence. Cold Spring Harbor Symposia on Quantitative Biology 33:809–822
    [Google Scholar]
  5. Kepes F., Kepes A. 1980; Synchronisation automatique de la croissance d’Escherichia coli . Annales de microbiologie 131A:3–16
    [Google Scholar]
  6. Kjeldgaard N. O. 1961; The kinetics of ribo-nucleic acid and protein formation in Salmonella typhimurium during the transition between different states of balanced growth. Biochimica et biophysica acta 49:64–73
    [Google Scholar]
  7. Kjeldgaard N. O., Maaløe O., Schaechter M. 1958; The transition between different physiological states during balanced growth of Salmonella typhimurium . Journal of General Microbiology 19:607–616
    [Google Scholar]
  8. Loeb A., McGrath B. E., Navre J. M., Pierucci O. 1978; Cell division during upshifts of Escherichia coli . Journal of Bacteriology 136:631–637
    [Google Scholar]
  9. Maaløe O., Kjeldgaard N. O. 1966 Control of Macromolecular Synthesis New York: W. A. Benjamin;
    [Google Scholar]
  10. Pace N. R. 1973; Structure and synthesis of the ribosomal ribonucleic acid of prokaryotes. Bacteriological Reviews 37:562–603
    [Google Scholar]
  11. Schleif R. 1967; Control of production of ribosomal protein. Journal of Molecular Biology 27:41–55
    [Google Scholar]
  12. Shepherd N., Churchward G., Bremer H. 1980; Synthesis and function of ribonucleic acid polymerase and ribosomes in Escherichia coli B/r after a nutritional shift-up. Journal of Bacteriology 143:1332–1334
    [Google Scholar]
  13. Sloan J. B., Urban J. E. 1976; Growth response of Escherichia coli to nutritional shift up. Journal of Bacteriology 128:302–308
    [Google Scholar]
  14. Sud I. J., Schaechter M. 1964; Dependence of the content of cell envelopes on the growth rate of Bacillus megaterium . Journal of Bacteriology 88:1612–1617
    [Google Scholar]
  15. Zaritsky A., Zabrovitz S. 1981; DNA synthesis in Escherichia coli during a nutritional shift-up. Molecular and General Genetics 181:564–566
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-131-3-677
Loading
/content/journal/micro/10.1099/00221287-131-3-677
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error