1887

Abstract

Differential extraction of Ca from the cytoplasmic and vacuolar pools of the yeast , using DEAE-dextran, revealed that most of the cellular Ca was bound, precipitated or sequestered within the vacuole. When the concentration of Ca in the medium was raised from 10 to 10 , cytoplasmic Ca homeostasis was maintained at 5·8 × 10 to 2·3 × 10 , whereas the vacuoles accumulated higher concentrations of Ca. The results indicate that the vacuoles function as a cytoplasmic Ca buffering system and as the major sequestering organelle for Ca. A respiratory-deficient mutant (°) displayed a similar intracellular distribution of Ca to the wild-type. When cells were permeabilized by DEAE-dextran the vacuoles were still capable of Ca uptake. This uptake proceeded without the addition of ATP or glucose in fresh preparations but required the addition of ATP after incubation of the permeabilized cells in buffered sorbitol for 2 h. The results are consistent with the proposed Ca/H antiport in the vacuolar membrane, which is driven by formed by the H-ATPase pumping H into the vacuole.

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/content/journal/micro/10.1099/00221287-131-3-623
1985-03-01
2022-01-18
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