1887

Abstract

The determinant(s) of gonococcal resistance to killing by human phagocytes has been extracted from outer membrane vesicles (OMV) of a phagocyte-resistant strain, BS4 (agar), with sodium cholate (1%, w/v). The extracts, like the OMV, nullified the effect of antiserum raised against whole BS4 (agar) to promote intracellular killing of the latter by human peripheral blood phagocytes. Fractionation of the extract on Sephadex G75 produced an active fraction with much less protein and lipopolysaccharide (LPS) than in the original extract. Furthermore, crude LPS prepared from the resistant gonococci was inactive. These results imply that the factor(s) promoting intracellular resistance is a protein. SDS-PAGE of the active fraction suggested that the factor was not a principal outer membrane protein nor one of three proteins previously thought to be associated with resistance. In contrast to a similar preparation from a phagocyte-susceptible strain, BSSH, the active fraction from BS4 (agar) showed faintly staining proteins in the regions of 20 and 60 kDal. When eluted from the gels, the former but not the latter neutralized the above effect of antisera, thus associating the 20 kDal protein(s) with resistance to intracellular killing.

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/content/journal/micro/10.1099/00221287-131-3-601
1985-03-01
2024-12-12
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References

  1. Cannon J. G., Black W. J., Nachamkin I., Stewart P. W. 1984; Monoclonal antibody that recognises an outer membrane antigen common to the pathogenic Neisseria species but not to most nonpathogenic Neisseria species. Infection and Immunity 43:994–999
    [Google Scholar]
  2. Casey S. G., Veale D. R., Smith H. 1979; Demonstration of intracellular growth of gonococci in human phagocytes using spectinomycin to kill extracellular organisms. Journal of General Microbiology 113:395–398
    [Google Scholar]
  3. Heckels J. E. 1977; The surface properties of Neisseria gonorrhoeae: isolation of the major components of the outer membrane. Journal of General Microbiology 99:333–341
    [Google Scholar]
  4. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 211:680–685
    [Google Scholar]
  5. Parsons N. J., Penn C. W., Veale D. R., Smith H. 1979; More than one antigen contributes to the immunogenicity of Neisseria gonorrhoeae in the guinea pig subcutaneous chamber model. Journal of General Microbiology 113:97–104
    [Google Scholar]
  6. Parsons N. J., Kwaasi A. A. A., Turner J. A., Veale D. R., Perera V. Y., Penn C. W., Smith H. 1981; Investigation of the determinants of the survival of Neisseria gonorrhoeae within human polymorphonuclear phagocytes. Journal of General Microbiology 127:103–112
    [Google Scholar]
  7. Parsons N. J., Kwaasi A. A. A., Perera V. Y., Patel P. V., Martin P. M. V., Smith H. 1982; Outer membrane proteins of Neisseria gonorrhoeae associated with survival within human polymorphonuclear phagocytes. Journal of General Microbiology 128:3077–3081
    [Google Scholar]
  8. Penn C. W., Sen D., Veale D. R., Parsons N. J., Smith H., Witt K. 1976; Morphological, biological and antigenic properties of Neisseria gonorrhoeae adapted to growth in guinea pig subcutaneous chambers. Journal of General Microbiology 97:35–43
    [Google Scholar]
  9. Penn C. W., Veale D. R., Smith H. 1977; Selection from gonococci grown in vitro of a colony type with some virulence properties of organisms adapted in vivo. Journal of General Microbiology 100:147–158
    [Google Scholar]
  10. Trevelyan W. E., Harrison J. S. 1952; A micromethod for determination of carbohydrate. Biochemical Journal 50:298–306
    [Google Scholar]
  11. Veale D. R., Smith H., Witt K., Marshall R. B. 1975; Differential ability of colonial types of Neisseria gonorrhoeae to produce infection and an inflammatory response in subcutaneous perforated plastic chambers in guinea pigs and rabbits. Journal of Medical Microbiology 8:325–335
    [Google Scholar]
  12. Veale D. R., Penn C. W., Sutton S., Smith H. 1978; The effect of specific antiserum on the resistance of Neisseria gonorrhoeae to intracellular killing by phagocytes of human blood. Journal of General Microbiology 106:129–136
    [Google Scholar]
  13. Veale D. R., Goldner M., Penn C. W., Ward J., Smith H. 1979; The intracellular survival and growth of gonococci in human phagocytes. Journal of General Microbiology 113:383–393
    [Google Scholar]
  14. Westphal O., Jann K. 1965; Lipopolysaccharide extraction with phenol water and further application of the procedure. Methods in Carbohydrate Chemistry 5:83–89
    [Google Scholar]
  15. Winstanley F. P., Blackwell C. C, Tan E. L., Patel P. V., Parsons N. J., Martin P. M. V., Smith H. 1984; Alteration of pyocin sensitivity pattern of Neisseria gonorrhoeae is associated with induced resistance to killing by human serum. Journal of General Microbiology 130:1303–1306
    [Google Scholar]
  16. Witt K., Veale D. R., Finch H., Penn C. W., Sen D, Smith H. 1976a; Resistance of Neisseria gonorrhoeae grown in vivo to ingestion and digestion by phagocytes of human buffy coat. Journal of General Microbiology 96:341–350
    [Google Scholar]
  17. Witt K., Veale D. R., Smith H. 1976b; Resistance to ingestion and digestion of Neisseria gonorrhoeae by phagocytes of human buffy coat. Journal of Medical Microbiology 9:1–12
    [Google Scholar]
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