1887

Abstract

Small heat-stable, acid-soluble proteins (HASP) have been isolated from nucleoids obtained from cell lysates of low ionic strength and lysozyme concentration. They were identified by their ability to bind homologous and heterologous native and denatured DNA. Four major species, of 8·5, 12, 23 and 26 kDal, were found. Their affinity for DNA was moderate as measured by the sensitivity to ionic strength of the DNA-protein complex (0·1-0·4--NaCl). Partial digestion by micrococcal nuclease of the ‘low ionic strength nucleoids’ released a DNA fragment of 80-120 bp. The data reported here indicate that small basic proteins, together with other components such as RNA, cations and polyamines, may be involved in the compaction of the prokaryotic genome.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-131-3-581
1985-03-01
2021-10-19
Loading full text...

Full text loading...

/deliver/fulltext/micro/131/3/mic-131-3-581.html?itemId=/content/journal/micro/10.1099/00221287-131-3-581&mimeType=html&fmt=ahah

References

  1. Alberts B., Herrick G. 1971 Methods in Enzymoiogy 21D:198–217
    [Google Scholar]
  2. Berthold V., Geider K. 1976; Interaction of DNA with DNA-binding proteins. European Journal of Biochemistry 71:443–449
    [Google Scholar]
  3. Bligh E. G., Dyer M. J. 1959; A rapid method of total lipid extraction and purification. Canadian Journal of Biochemistry and Physiology 37:911–917
    [Google Scholar]
  4. Brehm S. P., Le Hégarat F., Hoch J. A. 1974; Developmental modulations of deoxyribonucleic acid-binding proteins of Bacillus subtilis during sporulation stages. Journal of Bacteriology 120:1443–1450
    [Google Scholar]
  5. Bowen B., Steinberg J., Laemmli U.K., Wein-Traub H. 1980; The detection of DNA-binding proteins by protein blotting. Nucleic Acids Research 8:1–18
    [Google Scholar]
  6. Charret R., Guillen N., Fleury A. M., Le HéGarat F., Hirschbein L. 1980; Organization of the isolated Bacillus subtilis nucleoid. Biologie cellulaire 38:105–114
    [Google Scholar]
  7. Curiel-Quesada E., Setlow P. 1984; Cloning of a new low-molecular-weight spore-specific protein gene from Bacillus megaterium. Journal of Bacteriology 157:751–757
    [Google Scholar]
  8. Delius H., Worcel A. 1973; Electron micro-scopic studies on the folded chromosome of Escherichia coli. Cold Spring Harbor Symposia on Quantitative Biology 38:53–58
    [Google Scholar]
  9. Fleury-Guérout A. M. 1978; Caractérisation des proteines associées au nucléoïde de Bacillus subtilis. Thése de doctarat ̊ cycle Université Paris-Sud:
    [Google Scholar]
  10. Griffith J.D. 1976; Visualization of procaryotic DNA in a regularly condensed chromatine-like fiber. Proceedings of the National Academy of Sciences of the United States of America 73563–567
    [Google Scholar]
  11. Guillen N., Le Hégarat F., Fleury A. M., Hirschbein L. 1978; Folded chromosomes of vegetative Bacillus subtilis: composition and properties. Nucleid Acids Research 5:475–489
    [Google Scholar]
  12. Hamkalo B. A., Rattner J. B. 1980; Foldingup genes and chromosomes. Quaterly Review of Biology 55:409–417
    [Google Scholar]
  13. Hentschel C. C., Birnstiel M. L. 1981; The organization and expression of histones gene families. Cell 25:301–313
    [Google Scholar]
  14. Hirschbein L., Guillen N. 1982; Characterization, assay and use of isolated bacterial nucleoids. Methods of Biochemical Analysis 28:297–328
    [Google Scholar]
  15. Hirschbein L., Guillen N., Le Hégarat F. 1977; Isolation and characterization of nucleoids of B. subtilis during growth and sporulation. In Spore Research373–390 Barker A. N., Wolf J., Ellar D. J., Dring G. J., Gould G. W. London: Academic Press;
    [Google Scholar]
  16. Hübscher U., Lutz H., Kornberg A. 1980; Novel histone H2A-like protein of Escherichia coli. Proceedings of the National Academy of Sciences of the United States of America 775097–5101
    [Google Scholar]
  17. Huerre C., Le Hégarat J. C. 1979; Etude des clones de Bacillus subtilis résistants à la tetracycline obtenus par transformation avec des plasmides recombines in vitro. In Microbiologie 1979179 Paris: Du C.N.R.S.
    [Google Scholar]
  18. Imber R., Bachinger H., Bickle T. A. 1982; Purification and characterization of a small DNA-binding protein, HB, from Bacillus globigii. European Journal of Biochemistry 122:627–632
    [Google Scholar]
  19. Kavenoff R., Ryder O. A. 1976; Electron microscopy of membrane-associatedfolded chromo-somes of Escherichia coli. Chromosoma 55:13–25
    [Google Scholar]
  20. Kornberg R. D. 1977; Structure of chromatin. Annual Review of Biochemistry 46:931–954
    [Google Scholar]
  21. Laemmli U.K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature London 227:680–685
    [Google Scholar]
  22. Lathe R., Lecocq J. P., Resibois A. 1977; The fir A gene, a locus involved in the expression of rifampicin resistance in Escherichia coli. Molecular and General Genetics 154:43–51
    [Google Scholar]
  23. Lathe R., Buc H., Lecocq J. P., Bautz E. K. F. 1980; Procaryotic histone-like protein interacting with RNA polymerase. Proceedings of the National Academy of Sciences of the United States of America 773548–3552
    [Google Scholar]
  24. Le Hégarat F., Guillen N., Fleury A. M., Hirschbein L. 1978; Comparative studies on purified nucleoids of Bacillus subtilis during growth and sporulation. In Spores VII193–199 Chambliss G., Vary. J. C. Washington DC: American Society for Microbiology;
    [Google Scholar]
  25. Litman R. 1968; A deoxyribonucleic acid polymerase from Micrococcus luteus Micrococcus lysodeikti-cus isolated on deoxyribonucleic acid-cellulose. Journal of Biological Chemistry 243:6222–6233
    [Google Scholar]
  26. Materman E. C., Van Gool A. P. 1978; Compact Escherichia coli nucleoids in a highly supercoiled conformation. Journal of Bacteriology 135:703–706
    [Google Scholar]
  27. Nakayama T., Kurogi Y., Irikura M. 1981; Purification of polypeptide P6 with a molecular weight of 6,000 from the vegetative chromosomes of Bacillus subtilis. Journalof Bacteriology 145:953–957
    [Google Scholar]
  28. Noll M. 1974; Subunit structure of chromatin. Nature London 2511:249–251
    [Google Scholar]
  29. Noll M., Kornberg R. E. 1977; Action of micrococcal nuclease onchromatinand the location of histone H1. Journal of Molecular Biology 109:393–404
    [Google Scholar]
  30. RouviéRe-Yaniv J., Gros F. 1975; Characterization of a novel, low-molecular-weight DNA-binding protein from E. coli. Proceedings of the National Academy of Sciences of the United States of America 723428–3432
    [Google Scholar]
  31. RouviéRe-Yaniv J., Yaniv M., Germond J. E. 1979; Escherichia coli DNA-binding protein HUEC forms nucleosome-like structure with circular double stranded DNA. Cell 17:265–274
    [Google Scholar]
  32. Ryter A., Chang A. 1975; Localization of transcribing genes in the bacterial cell by means of high resolution autoradiography. Journal of Molecular Biology 98:797–810
    [Google Scholar]
  33. Saito H., Muira K. 1963; Preparation of transforming deoxyribonucleic acid by phenol treat-ment. Biochimica et biophysica acta 72:619–629
    [Google Scholar]
  34. Schaeffer P., Ionesco H., Ryter A., Balassa G. 1965; La sporulation de Bacillus subtilis, étude génétique et physiologique. Colloque International du Center Nationalpour la Recherche Scientifique 124:553–563
    [Google Scholar]
  35. Sjåstad K., Fadnes P., Kruger P. G., Lossius I., Kleppe K. 1982; Isolation, properties and nucleo-lytic degradation of chromatin from Escherichia coli. Journal of General Microbiology 128:3037–3050
    [Google Scholar]
  36. Stonington G., Pettijohn D. 1971; The folded genome of Escherichia coli isolated in a protein-DNA-RNA complex. Proceedings of the National Academy of Sciences ofthe United States of America 686–9
    [Google Scholar]
  37. Suryanarayana T., Subramanian A. R. 1978; Specific association of two homologous DNA-binding proteins to the native 30 S ribosomal subunits of Escherichia coli. Biochimica et biophysica acta 520:342–357
    [Google Scholar]
  38. Towbin H., Staehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacryl-amide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences of the United States America 764350–4354
    [Google Scholar]
  39. Varshavsky A. J., Nedospasov S. A., Bakayev V. V., Bakayeva T. G., Georgiev G. P. 1977; Histone-like proteins in the purified Escherichia coli deoxyribonucleoprotein. Nucleic Acids Research 4:2725–2745
    [Google Scholar]
  40. Worcel A., Burgi E. 1974; Properties of the membrane-attached form of the folded chromosome of Escherichia coli. Journal of Molecular Biology 82:92–105
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-131-3-581
Loading
/content/journal/micro/10.1099/00221287-131-3-581
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error