Characterization of DNA Fragments Encoding Fimbriae of the Uropathogenic Strain KS71 Free

Abstract

Recombinant plasmids were constructed that expressed the KS71A, KS71B and KS71C fimbrial antigens of the pyelonephritogenic strain KS71 (04:K12) in HB101. The KS71C-encoding genes were located on a 6·4 kb dIII–I fragment obtained from the recombinant cosmid pKTH145 that expresses this antigen. Spontaneous KS71C mutants were isolated that contained a 0·8 kb insert in a specific restriction fragment of KS71C-encoding recombinant plasmids. The KS71 B-encoding segment was located on a 11·5 kb deletable DNA fragment of recombinant cosmid pKTH144. A DNA fragment encoding the KS71A fimbria was obtained on a 12 kb RI fragment of the recombinant cosmid expressing this antigen in HB101 and closely resembled the KS71B-encoding fragment. In the recombinant cosmid, the KS71 B-expressing region was flanked by homologous DNA segments. A similar stretch of DNA was found close to the KS71A-expressing DNA region.

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1985-03-01
2024-03-29
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